A Feulgen microspectrophotometric study of the DNA content of essential fatty acid-deficient rat pancreas treated with nitrosomethylurea. Cell Mol Biol 1990;36(5):547-55
Date
01/01/1990Pubmed ID
2073679Scopus ID
2-s2.0-0025668851 (requires institutional sign-in at Scopus site) 3 CitationsAbstract
Microspectrophotometric DNA measurements in exocrine pancreas of essential fatty acid-deficient (EFAD) and EFA-sufficient (EFAS) rats which received a single intraperitoneal injection of the carcinogen nitrosomethylurea (NMU) or saline (SAL) was the subject of the present report. The DNA content of acinar pancreatic cells of SAL-injected EFAD and EFAS rats was diploid. NMU-induced pancreatic focal acinar cell hyperplasia (FACH) had one main cell population with a diploid content, whereas in the intervening parenchyma there were diploid and tetraploid cells. The number of tetraploid cells was smaller in EFAD rat pancreas than in EFAS indicating a diet dependent effect. NMU-induced FACH had a diploid distribution pattern indicating that cells are in a G1, quiescent phase, contrasting with AZA-induced similar lesions which showed an abnormal ploidy. It remains to be established whether DNA phenotypic traits of NMU and AZA induced FACH reflect the neoplastic potentials of both types of lesions. The decreased number of tetraploid cells in EFAD rat pancreas is in keeping with data indicating a promoting effect of the EFA linoleic and arachidonic acids on growth rate of certain cell populations in vitro.
Author List
Urrutia RA, Rivolta CM, Valentich MA, Monis BAuthor
Raul A. Urrutia MD Center Director, Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsDNA
Fatty Acids, Essential
Female
Methylnitrosourea
Microspectrophotometry
Pancreas
Pregnancy
Rats
Rats, Inbred Strains