Faculty Collaboration Database

Secretion of inhibin beta A by endoderm cultured from early embryonic chicken. Dev Biol 1991 Jul;146(1):242-5

Date

07/01/1991

Pubmed ID

2060706

DOI

10.1016/0012-1606(91)90464-e

Scopus ID

2-s2.0-0025762279 (requires institutional sign-in at Scopus site)   38 Citations

Abstract

Although several reports have indicated a role for endoderm in the regulation of heart development, the mechanism remains unknown. To begin characterization of endoderm-secreted proteins, explants from postgastrulation (Hamburger-Hamilton stage 5/6-8) chicken embryos were cultured in defined medium. Fluorography of SDS-PAGE gels revealed a pattern of synthesized, secreted proteins that was independent of time in culture or embryonic stage when explants were removed. Approximately 10 labeled bands were detected, the most prominent of which migrated at 17, 25, and 200 kDa. ELISA analysis revealed that while acidic and basic fibroblast growth factor-like antigens were barely detectable, fibronectin and inhibin beta A were very reactive. Western blot analysis verified the presence of fibronectin and, most remarkably, inhibin beta A, activin dimers of which have recently been implicated in Xenopus mesoderm induction (Smith, Price, Van Nimmen, and Huylebrock (1990). Nature 345, 729.)

Author List

Kokan-Moore NP, Bolender DL, Lough J

Author

John W. Lough PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Activins
Animals
Chick Embryo
Culture Media
Culture Techniques
Endoderm
Fibronectins
Gastrula
Inhibins
Peptides
Prostatic Secretory Proteins