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Nuclear microinjection to assess how heterologously expressed proteins impact Ca2+ signals in Xenopus oocytes. Cold Spring Harb Protoc 2013 Mar 01;2013(3)

Date

03/05/2013

Pubmed ID

23457340

Pubmed Central ID

PMC4021487

DOI

10.1101/pdb.prot072785

Scopus ID

2-s2.0-84874687582   5 Citations

Abstract

The Xenopus oocyte is frequently used for heterologous expression and for studying the spatiotemporal patterning of Ca(2+) signals. Here, we outline a protocol for nuclear microinjection of the Xenopus oocyte for the purpose of studying how subsequently expressed proteins impact intracellular Ca(2+) signals evoked by inositol trisphosphate (InsP3). Injected oocytes can easily be identified by reporter technologies and the impact of heterologously expressed proteins on the generation and properties of InsP3-evoked Ca(2+) signals can be resolved using caged InsP3 and fluorescent Ca(2+) indicators.

Author List

Lin-Moshier Y, Marchant JS

Author

Jonathan S. Marchant PhD Chair, Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Calcium
Inositol Phosphates
Microinjections
Molecular Biology
Oocytes
Recombinant Proteins
Signal Transduction
Xenopus
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a