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α-Adrenergic Receptors Function Within Hetero-Oligomeric Complexes With Atypical Chemokine Receptor 3 and Chemokine (C-X-C motif) Receptor 4 in Vascular Smooth Muscle Cells. J Am Heart Assoc 2017 Aug 17;6(8)



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Scopus ID

2-s2.0-85030676850   8 Citations


BACKGROUND: Recently, we provided evidence that α-adrenergic receptors (ARs) in vascular smooth muscle are regulated by chemokine (C-X-C motif) receptor (CXCR) 4 and atypical chemokine receptor 3 (ACKR3). While we showed that CXCR4 controls α-ARs through formation of heteromeric receptor complexes in human vascular smooth muscle cells (hVSMCs), the molecular basis underlying cross-talk between ACKR3 and α-ARs is unknown.

METHODS AND RESULTS: We show that ACKR3 agonists inhibit inositol trisphosphate production in hVSMCs on stimulation with phenylephrine. In proximity ligation assays and co-immunoprecipitation experiments, we observed that recombinant and endogenous ACKR3 form heteromeric complexes with α-AR. While small interfering RNA knockdown of ACKR3 in hVSMCs reduced α-AR:ACKR3, CXCR4:ACKR3, and α-AR:CXCR4 complexes, small interfering RNA knockdown of CXCR4 reduced α-AR:ACKR3 heteromers. Phenylephrine-induced inositol trisphosphate production from hVSMCs was abolished after ACKR3 and CXCR4 small interfering RNA knockdown. Peptide analogs of transmembrane domains 2/4/7 of ACKR3 showed differential effects on heteromerization between ACKR3, α-AR, and CXCR4. While the transmembrane domain 2 peptide interfered with α-AR:ACKR3 and CXCR4:ACKR3 heteromerization, it increased heteromerization between CXCR4 and α-AR. The transmembrane domain 2 peptide inhibited ACKR3 but did not affect α-AR in β-arrestin recruitment assays. Furthermore, the transmembrane domain 2 peptide inhibited phenylephrine-induced inositol trisphosphate production in hVSMCs and attenuated phenylephrine-induced constriction of mesenteric arteries.

CONCLUSIONS: α-ARs form hetero-oligomeric complexes with the ACKR3:CXCR4 heteromer, which is required for α-AR function, and activation of ACKR3 negatively regulates α-ARs. G protein-coupled receptor hetero-oligomerization is a dynamic process, which depends on the relative abundance of available receptor partners. Endogenous α-ARs function within a network of hetero-oligomeric receptor complexes.

Author List

Albee LJ, Eby JM, Tripathi A, LaPorte HM, Gao X, Volkman BF, Gaponenko V, Majetschak M


Brian F. Volkman PhD Professor in the Biochemistry department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adenosine Triphosphate
Adrenergic alpha-1 Receptor Agonists
Dose-Response Relationship, Drug
HEK293 Cells
Multiprotein Complexes
Muscle, Smooth, Vascular
Myocytes, Smooth Muscle
Protein Binding
Protein Interaction Domains and Motifs
RNA Interference
Receptor Cross-Talk
Receptors, Adrenergic, alpha-1
Receptors, CXCR
Receptors, CXCR4
Signal Transduction
jenkins-FCD Prod-444 eb4ebd1a08581aba961d3befd3b851a3c3ec6b46