Restriction maps and restriction fragment length polymorphisms of the human 21-hydroxylase genes. Biochem Biophys Res Commun 1986 Apr 29;136(2):722-9
Date
04/29/1986Pubmed ID
3010994DOI
10.1016/0006-291x(86)90499-7Scopus ID
2-s2.0-0022448105 (requires institutional sign-in at Scopus site) 27 CitationsAbstract
Restriction maps were constructed for the two human 21-hydroxylase genes (21-OHA and 21-OHB) by using DNA from subjects homozygous for a deletion of each gene. Comparing the patterns of these two genes, a KpnI restriction site occurred in the 21-OHA gene in place of a TaqI site in the 21-OHB gene about 1-kb from the 5' end of the gene, and an extra EcoRI site was located 500 bp 5' to the common EcoRI site. The DNA of fourteen unrelated normal subjects was digested with nine restriction endonucleases (AccI, BamHI, BgIII, EcoRI, HindIII, KpnI, MspI, SacI and TaqI). Restriction fragment length polymorphisms were found with EcoRI, HindIII and AccI that resulted from polymorphic endonuclease sites outside the genes.
Author List
Donohoue PA, Jospe N, Migeon CJ, McLean RH, Bias WB, White PC, Van Dop CMESH terms used to index this publication - Major topics in bold
Adrenal Hyperplasia, CongenitalDNA
DNA Restriction Enzymes
Female
HLA Antigens
HLA-DR Antigens
Histocompatibility Antigens Class II
Homozygote
Humans
Male
Nucleic Acid Hybridization
Phenotype
Polymorphism, Genetic
Steroid 21-Hydroxylase
Steroid Hydroxylases