High Content Screening of Mammalian Primary Cortical Neurons. Methods Mol Biol 2018;1683:293-304
Date
10/31/2017Pubmed ID
29082499DOI
10.1007/978-1-4939-7357-6_17Scopus ID
2-s2.0-85032623378 (requires institutional sign-in at Scopus site) 1 CitationAbstract
High Content Screening (HCS) can be used to analyze the morphology of neuronal primary cultures on a large scale. When used in the field of neuronal regeneration this approach allows the screening of hundreds or thousands of perturbagens, such as miRNAs, cDNAs, or compounds, for their ability to induce neuronal growth. One of the most important steps while designing these kinds of experiments is the choice of the correct neuronal model. Testing the correct neuronal type is critical to obtain results that are biologically significant and that can later be translated to a clinical setting. For example, if the goal is identifying possible therapies for Spinal Cord Injury (SCI), a challenging target is the neuronal projection from the motor cortex to the spinal cord, the corticospinal tract. Here, we describe the experimental protocols that can be used to produce primary cortical culture from young rat cortices, electroporate the neurons to study the effect of altered gene expression on neurite growth, and immunostain to measure neurite growth parameters.
Author List
Motti D, Blackmore M, Bixby JL, Lemmon VPAuthor
Murray Blackmore PhD Assistant Professor in the School of Allied Health department at Marquette UniversityMESH terms used to index this publication - Major topics in bold
AnimalsCerebral Cortex
Drug Discovery
Gene Expression Regulation
High-Throughput Screening Assays
Nerve Regeneration
Neurons
Phenotype
Rats
