Supercomplex-associated Cox26 protein binds to cytochrome c oxidase. Biochim Biophys Acta 2016 Jul;1863(7 Pt A):1643-52
Date
04/20/2016Pubmed ID
27091403Pubmed Central ID
PMC7140176DOI
10.1016/j.bbamcr.2016.04.012Scopus ID
2-s2.0-84986292138 (requires institutional sign-in at Scopus site) 26 CitationsAbstract
Here we identified a hydrophobic 6.4kDa protein, Cox26, as a novel component of yeast mitochondrial supercomplex comprising respiratory complexes III and IV. Multi-dimensional native and denaturing electrophoretic techniques were used to identify proteins interacting with Cox26. The majority of the Cox26 protein was found non-covalently bound to the complex IV moiety of the III-IV supercomplexes. A population of Cox26 was observed to exist in a disulfide bond partnership with the Cox2 subunit of complex IV. No pronounced growth phenotype for Cox26 deficiency was observed, indicating that Cox26 may not play a critical role in the COX enzymology, and we speculate that Cox26 may serve to regulate or support the Cox2 protein. Respiratory supercomplexes are assembled in the absence of the Cox26 protein, however their pattern slightly differs to the wild type III-IV supercomplex appearance. The catalytic activities of complexes III and IV were observed to be normal and respiration was comparable to wild type as long as cells were cultivated under normal growth conditions. Stress conditions, such as elevated temperatures resulted in mild decrease of respiration in non-fermentative media when the Cox26 protein was absent.
Author List
Strecker V, Kadeer Z, Heidler J, Cruciat CM, Angerer H, Giese H, Pfeiffer K, Stuart RA, Wittig IAuthor
Rosemary Stuart PhD Professor in the Biology department at Marquette UniversityMESH terms used to index this publication - Major topics in bold
CatalysisDisulfides
Electron Transport Complex IV
Electrophoresis
Enzyme Stability
Hydrophobic and Hydrophilic Interactions
Mitochondria
Mitochondrial Membranes
Models, Molecular
Molecular Weight
Oxygen Consumption
Protein Binding
Protein Denaturation
Protein Subunits
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Temperature
