Optical imaging for the assessment of hepatocyte metabolic state in ischemia and reperfusion injuries. Biomed Opt Express 2017 Oct 01;8(10):4419-4426
Date
10/31/2017Pubmed ID
29082074Pubmed Central ID
PMC5654789DOI
10.1364/BOE.8.004419Abstract
Deterioration in mitochondrial function leads to hepatic ischemia and reperfusion injury (IRI) in liver surgery and transplantation. 3D optical cryoimaging was used to measure the levels of mitochondrial coenzymes NADH and FAD, and their redox ratio (NADH/FAD) gave a quantitative marker for hepatocyte oxidative stress during IRI. Using a rat model, five groups were compared: control, ischemia for 60 or 90 minutes (Isc60, Isc90), ischemia for 60 or 90 minutes followed by reperfusion of 24 hours (IRI60, IRI90). Ischemia alone did not cause a significant increase in the redox ratio; however, the redox ratio in both IRI60 and IRI90 groups was significantly decreased by 29% and 71%, respectively. A significant correlation was observed between the redox ratio and other markers of injury such as serum aminotransferase levels and the tissue ATP level. The mitochondrial redox state can be successfully measured using optical cryoimaging as a quantitative marker of hepatic IR injury.
Author List
la Cour MF, Mehrvar S, Kim J, Martin A, Zimmerman MA, Hong JC, Ranji MAuthors
Joohyun Kim MD, PhD Associate Professor in the Surgery department at Medical College of WisconsinMichael A. Zimmerman MD, FACS Professor in the Surgery department at Medical College of Wisconsin
