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Optical imaging for the assessment of hepatocyte metabolic state in ischemia and reperfusion injuries. Biomed Opt Express 2017 Oct 01;8(10):4419-4426

Date

10/31/2017

Pubmed ID

29082074

Pubmed Central ID

PMC5654789

DOI

10.1364/BOE.8.004419

Abstract

Deterioration in mitochondrial function leads to hepatic ischemia and reperfusion injury (IRI) in liver surgery and transplantation. 3D optical cryoimaging was used to measure the levels of mitochondrial coenzymes NADH and FAD, and their redox ratio (NADH/FAD) gave a quantitative marker for hepatocyte oxidative stress during IRI. Using a rat model, five groups were compared: control, ischemia for 60 or 90 minutes (Isc60, Isc90), ischemia for 60 or 90 minutes followed by reperfusion of 24 hours (IRI60, IRI90). Ischemia alone did not cause a significant increase in the redox ratio; however, the redox ratio in both IRI60 and IRI90 groups was significantly decreased by 29% and 71%, respectively. A significant correlation was observed between the redox ratio and other markers of injury such as serum aminotransferase levels and the tissue ATP level. The mitochondrial redox state can be successfully measured using optical cryoimaging as a quantitative marker of hepatic IR injury.

Author List

la Cour MF, Mehrvar S, Kim J, Martin A, Zimmerman MA, Hong JC, Ranji M

Authors

Joohyun Kim MD, PhD Associate Professor in the Surgery department at Medical College of Wisconsin
Michael A. Zimmerman MD, FACS Professor in the Surgery department at Medical College of Wisconsin




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