Medical College of Wisconsin
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Cone myoid elongation involves unidirectional microtubule movement mediated by dynein-1. Mol Biol Cell 2018 01 15;29(2):180-190

Date

11/17/2017

Pubmed ID

29142075

Pubmed Central ID

PMC5909930

DOI

10.1091/mbc.E17-08-0525

Scopus ID

2-s2.0-85040967232   1 Citation

Abstract

Teleosts and amphibians exhibit retinomotor movements, morphological changes in photoreceptors regulated by light and circadian rhythms. Cone myoid elongation occurs during dark adaptation, leading to the positioning of the cone outer segment closer to the retinal pigment epithelium. Although it has been shown that microtubules are essential for cone myoid elongation, the underlying mechanism has not been established. In this work, we generated a transgenic line of zebrafish expressing a photoconvertible form of α-tubulin (tdEOS-tubulin) specifically in cone photoreceptors. Using superresolution structured illumination microscopy in conjunction with both pharmacological and genetic manipulation, we show that cytoplasmic dynein-1, which localizes to the junction between the ellipsoid and myoid, functions to shuttle microtubules from the ellipsoid into the myoid during the course of myoid elongation. We propose a novel model by which stationary complexes of cytoplasmic dynein-1 are responsible for the shuttling of microtubules between the ellipsoid and myoid is the underlying force for the morphological change of myoid elongation.

Author List

Lewis TR, Zareba M, Link BA, Besharse JC

Author

Brian A. Link PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Animals, Genetically Modified
Cytoplasmic Dyneins
Dark Adaptation
Microtubules
Nocodazole
Pigment Epithelium of Eye
Retinal Cone Photoreceptor Cells
Tubulin Modulators
Zebrafish
jenkins-FCD Prod-486 e3098984f26de787f5ecab75090d0a28e7f4f7c0