Faculty Collaboration Database

Analysis of transforming growth factor beta receptor binding in embryonic, fetal, and adult rabbit fibroblasts. J Pediatr Surg 1989 Aug;24(8):784-8

Date

08/01/1989

Pubmed ID

2549233

DOI

10.1016/s0022-3468(89)80537-8

Scopus ID

2-s2.0-0024404093 (requires institutional sign-in at Scopus site)   16 Citations

Abstract

Adult wound repair traits including inflammation, fibroplasia, and collagen deposition are not seen at fetal wound sites. This observation raised questions about regulatory mechanisms extant in fetal healing. Transforming growth factor beta (TGF-beta) is an important regulatory polypeptide known to orchestrate fibroplasia and collagen synthesis during adult wound repair. Previous studies have suggested that the wounded rabbit fetus is capable of responding with these adult characteristics if provided with exogenous TGF-beta. In order to test whether the observed in vivo effects of TGF-beta in the rabbit fetus might be due to a direct effect on the fibroblast, TGF-beta receptor binding characteristics of early passage cultured embryonic (14 days' gestation), fetal (24 days' gestation), and adult rabbit fibroblasts were studied by flow cytometry. Experiments were carried out using fluorescein-conjugated TGF-beta (F-TGF-beta) with analysis on an EPICS V flow cytometer. F-TGF-beta was incubated with each of the three fibroblast types at 37 degrees C after which time the cells were washed twice and analyzed with a minimum of 10(5) cells for each data point. F-TGF-beta bound rapidly and reversibly to the embryonic, fetal, and adult fibroblasts with saturation being achieved at 1 nmol/L for fetal and adult cells, and 8 nmol/L in the embryonic fibroblasts. Saturating concentrations of F-TGF-beta yielded mean channel numbers (a function of relative amounts of F-TGF-beta-bound) of 172, 114, and 97 for embryonic, fetal, and adult cells, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Author List

Durham LA 3rd, Krummel TM, Cawthorn JW, Thomas BL, Diegelmann RF

Author

Lucian A. Durham MD, PhD Associate Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cell Differentiation
Embryonic and Fetal Development
Fetus
Fibroblasts
Flow Cytometry
Rabbits
Receptors, Cell Surface
Receptors, Transforming Growth Factor beta
Transforming Growth Factors
Wound Healing