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Heterologous Protein Expression in the Xenopus Oocyte. Cold Spring Harb Protoc 2018 04 02;2018(4)

Date

12/07/2017

Pubmed ID

29208643

Pubmed Central ID

PMC6139252

DOI

10.1101/pdb.prot096990

Scopus ID

2-s2.0-85044950019   1 Citation

Abstract

The Xenopus oocyte is a specialized single cell of colossal size (>1 mm diameter) that is highly amenable for microinjection and a stalwart model for heterologous expression. Oocytes are easily obtainable, robust in vitro, and faithfully express injected constructs. Their large size translational capacity provides a huge canvas for observing and recording integrated cellular responses-from studies of single molecules within single cells to medium-throughput drug-screening applications. Most eukaryotic promoters suffice for Xenopus expression, and the oocyte can functionally express proteins from many diverse organisms. This protocol provides a basic introduction for scientists keen to perform nuclear microinjections of cDNA constructs. These are easy methods to master, do not require elaborate equipment, and make accessible a wonderful model cell system for studying signaling, transport, cell architecture, and protein function.

Author List

Marchant JS

Author

Jonathan S. Marchant PhD Chair, Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
DNA, Complementary
Female
Gene Expression
Microinjections
Molecular Biology
Oocytes
Xenopus laevis
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a