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Direct observation of singlet oxygen production by merocyanine 540 associated with phosphatidylcholine liposomes. J Biol Chem 1988 Nov 25;263(33):17247-50

Date

11/25/1988

Pubmed ID

3182846

Scopus ID

2-s2.0-0024242683 (requires institutional sign-in at Scopus site)   41 Citations

Abstract

The production of singlet molecular oxygen (1O2) by the photosensitizing dye merocyanine 540 (MC540) bound to phosphatidylcholine liposomes has been demonstrated by direct detection of 1O2 luminescence at 1268 nm. 1O2 phosphorescence emission was enhanced in deuterated buffer and upon saturation of the sample with oxygen and could be quenched by the addition of sodium azide to the external medium. No 1O2 luminescence was detected in nitrogen-saturated samples, in the absence of dye, or with MC540 in aqueous solution. Photobleaching of liposome-bound MC540 was also observed to be dependent on oxygen concentration. These studies are consistent with 1O2 intermediacy in the mechanism of MC540-mediated photosensitization.

Author List

Feix JB, Kalyanaraman B, Chignell CF, Hall RD

Authors

Jimmy B. Feix PhD Professor in the Biophysics department at Medical College of Wisconsin
Balaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Dimyristoylphosphatidylcholine
Fluorescent Dyes
Kinetics
Liposomes
Luminescent Measurements
Oxygen
Photochemistry
Pyrimidinones
Singlet Oxygen