Faculty Collaboration Database

Prostate-derived factor as a paracrine and autocrine factor for the proliferation of androgen receptor-positive human prostate cancer cells. Prostate 2007 Apr 01;67(5):557-71

Date

01/16/2007

Pubmed ID

17221842

DOI

10.1002/pros.20551

Scopus ID

2-s2.0-34047209514 (requires institutional sign-in at Scopus site)   64 Citations

Abstract

BACKGROUND: The expression of prostate-derived factor (PDF) is significantly elevated in human prostate tumors. We investigate the functional role and signaling of PDF in androgen receptor (AR)-positive human prostate cancer cells.

METHODS: Transient or stable expression of PDF by cDNA transfection, antisense-mediated gene silencing, media conditioned by PDF-elevated cells, and antibody (Ab) neutralization were employed.

RESULTS: Elevated endogenous and exogenous expression of PDF and treatment of PDF-enriched media were associated with increased proliferation and clonogenic growth of the cells. On the contrary, knockdown of PDF or addition of PDF neutralizing Ab resulted in diminished proliferation and reduced anchorage-independent growth. Further, ERK1/2 and p90RSK, but not Smad2/3, were activated in PDF-elevated cells as well as in cells treated with PDF-enriched media, while inhibition of ERK1/2 decreased the growth of those cells.

CONCLUSION: PDF promotes AR-positive prostate tumor progression through upregulating cell proliferation via ERK1/2 signal pathway.

Author List

Chen SJ, Karan D, Johansson SL, Lin FF, Zeckser J, Singh AP, Batra SK, Lin MF

MESH terms used to index this publication - Major topics in bold

Blotting, Western
Bone Morphogenetic Proteins
Cell Adhesion
Cell Growth Processes
Cell Line, Tumor
Gene Silencing
Growth Differentiation Factor 15
Humans
Male
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3
Prostatic Neoplasms
Receptors, Androgen
Ribosomal Protein S6 Kinases, 90-kDa
Signal Transduction
Transfection
Transforming Growth Factor beta