Overlapping role of dynamin isoforms in synaptic vesicle endocytosis. Neuron 2011 Jun 23;70(6):1100-14
Date
06/22/2011Pubmed ID
21689597Pubmed Central ID
PMC3190241DOI
10.1016/j.neuron.2011.04.031Scopus ID
2-s2.0-79959316429 (requires institutional sign-in at Scopus site) 159 CitationsAbstract
The existence of neuron-specific endocytic protein isoforms raises questions about their importance for specialized neuronal functions. Dynamin, a GTPase implicated in the fission reaction of endocytosis, is encoded by three genes, two of which, dynamin 1 and 3, are highly expressed in neurons. We show that dynamin 3, thought to play a predominantly postsynaptic role, has a major presynaptic function. Although lack of dynamin 3 does not produce an overt phenotype in mice, it worsens the dynamin 1 KO phenotype, leading to perinatal lethality and a more severe defect in activity-dependent synaptic vesicle endocytosis. Thus, dynamin 1 and 3, which together account for the overwhelming majority of brain dynamin, cooperate in supporting optimal rates of synaptic vesicle endocytosis. Persistence of synaptic transmission in their absence indicates that if dynamin plays essential functions in neurons, such functions can be achieved by the very low levels of dynamin 2.
Author List
Raimondi A, Ferguson SM, Lou X, Armbruster M, Paradise S, Giovedi S, Messa M, Kono N, Takasaki J, Cappello V, O'Toole E, Ryan TA, De Camilli PAuthor
Xuelin Lou PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBrain
Cells, Cultured
Dynamin I
Dynamin III
Endocytosis
Mice
Mice, Knockout
Neurons
Protein Isoforms
Synaptic Vesicles