Faculty Collaboration Database

Urinary exosomal expression of activator of G protein signaling 3 in polycystic kidney disease. BMC Res Notes 2018 Jun 07;11(1):359

Date

06/09/2018

Pubmed ID

29880041

Pubmed Central ID

PMC5992714

DOI

10.1186/s13104-018-3467-6

Scopus ID

2-s2.0-85048187272 (requires institutional sign-in at Scopus site)   19 Citations

Abstract

OBJECTIVE: PKD is a genetic disease that is characterized by abnormally proliferative epithelial cells in the kidney and liver. Urinary exosomes have been previously examined as a source of unique proteins that may be used to diagnose and monitor the progression of PKD. Previous studies by our group have shown that AGS3, which is a receptor-independent regulator G-proteins, was markedly upregulated in RTECs during kidney injury including PKD. In this study, our goal was to determine whether AGS3 could be measured in exosomes using animals and humans with PKD.

RESULTS: In our study, urinary exosomes were isolated from PCK rats and the control Sprague-Dawley (SD) rats. AGS3 expression was significantly increased (P < 0.05) in PKD versus SD rats at 16 weeks of age. This increase was detectable in a time-dependent manner from 8 weeks of age and peaked at ~ 16-20 weeks (length of study). Similarly, in exosomes from human urine samples with PKD, AGS3 expression was significantly increased (P < 0.05) compared to healthy human controls where AGS3 was largely undetectable. In conclusion, the detection of AGS3 in urinary exosomes may be a novel biomarker for PKD, and provide new insight into the biology of tubular epithelial cell function during cystic disease progression.

Author List

Keri KC, Regner KR, Dall AT, Park F

Authors

Aaron Dall MD Associate Professor in the Medicine department at Medical College of Wisconsin
Kevin R. Regner MD Interim Chair, Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Aged
Animals
Biomarkers
Carrier Proteins
Disease Models, Animal
Exosomes
Female
Guanine Nucleotide Dissociation Inhibitors
Humans
Male
Middle Aged
Polycystic Kidney Diseases
Rats
Rats, Sprague-Dawley
Young Adult