Faculty Collaboration Database

Flavobacterium johnsoniae SprA is a cell surface protein involved in gliding motility. J Bacteriol 2007 Oct;189(19):7145-50

Date

07/24/2007

Pubmed ID

17644580

Pubmed Central ID

PMC2045224

DOI

10.1128/JB.00892-07

Scopus ID

2-s2.0-34948891544   30 Citations

Abstract

Flavobacterium johnsoniae cells glide rapidly over surfaces by an unknown mechanism. Transposon-induced sprA mutants formed nonspreading colonies on agar, and the cells examined in wet mounts were deficient in attachment to surfaces and were almost completely nonmotile. Exposure of intact cells to proteinase K cleaved the 270-kDa SprA into several large peptides, suggesting that it is partially exposed on the cell surface.

Author List

Nelson SS, Glocka PP, Agarwal S, Grimm DP, McBride MJ

Author

Padden P. Glocka MD Assistant Professor in the Emergency Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Bacterial Proteins
Bacteriophages
Chitin
Cloning, Molecular
DNA Transposable Elements
Endopeptidase K
Flavobacterium
Gene Expression Regulation, Bacterial
Genetic Complementation Test
Membrane Proteins
Molecular Sequence Data
Mutagenesis, Insertional
Mutation
Sequence Analysis, DNA