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Clinical Evaluation of the iCubate iC-GPC Assay for Detection of Gram-Positive Bacteria and Resistance Markers from Positive Blood Cultures. J Clin Microbiol 2018 Sep;56(9)

Date

06/15/2018

Pubmed ID

29899000

Pubmed Central ID

PMC6113461

DOI

10.1128/JCM.00485-18

Scopus ID

2-s2.0-85052491577 (requires institutional sign-in at Scopus site)   9 Citations

Abstract

The iC-GPC Assay (iCubate, Huntsville, AL) is a qualitative multiplex test for the detection of five of the most common Gram-positive bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Enterococcus faecalis, and Enterococcus faecium) responsible for bacterial bloodstream infections, performed directly from positive blood cultures. The assay also detects the presence of the mecA, vanA, and vanB resistance determinants. This study comparatively evaluated the performance of the iC-GPC Assay against the Verigene Gram-positive blood culture (BC-GP) assay (Luminex Corp., Austin, TX) for 1,134 patient blood culture specimens positive for Gram-positive cocci. The iC-GPC Assay had an overall percent agreement with the BC-GP assay of 95.5%. Discordant specimens were further analyzed by PCR and a bidirectional sequencing method. The results indicate that the iC-GPC Assay together with the iCubate system is an accurate and reliable tool for the detection of the five most common Gram-positive bacteria and their resistance markers responsible for bloodstream infections.

Author List

Granato PA, Unz MM, Widen RH, Silbert S, Young S, Heflin KL, Conover MS, Buchan BW, Ledeboer NA

Authors

Blake W. Buchan PhD Professor in the Pathology department at Medical College of Wisconsin
Nathan A. Ledeboer PhD Vice Chair, Professor in the Pathology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Bacteremia
Bacteriological Techniques
Blood Culture
Drug Resistance, Bacterial
Genes, Bacterial
Gram-Positive Bacteria
Gram-Positive Bacterial Infections
Humans
Molecular Diagnostic Techniques
Polymerase Chain Reaction