Differential impairment of vascularization and angiogenesis in bisphosphonate-associated osteonecrosis of the jaw-related mucoperiosteal tissue. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011 Aug;112(2):216-21
Date
06/15/2011Pubmed ID
21664154DOI
10.1016/j.tripleo.2011.02.028Scopus ID
2-s2.0-79960285325 (requires institutional sign-in at Scopus site) 49 CitationsAbstract
OBJECTIVES: Impaired vascularization in the etiopathology of aminobisphosphonate-associated osteonecrosis of the jaw (BONJ) is assumed, but evidence is lacking. This immunohistochemical study differentiated vascularization and angiogenesis in BONJ-adjacent mucoperiosteal tissue.
STUDY DESIGN: Twenty BONJ (after zoledronate treatment) and 20 control mucoperiosteal tissue samples were processed with an autostaining-based alkaline phosphatase-antialkaline phosphatase staining kit. Vascularization was assessed by CD31 staining and angiogenesis-related neovessels by CD105 staining. The ratio of stained capillary area to total area of visible field was assessed. Statistics included Bonferroni adjustment.
RESULTS: CD31-stained microvessels were detected in each section and CD105-stained neovessels in each control. BONJ-adjacent mucoperiosteal tissue showed significantly fewer CD105-positive vessels in capillary areas (P < .05) than control samples. CD31-stained capillary area was not significantly reduced in mucoperiosteal BONJ-samples.
CONCLUSIONS: Angiogenesis is impaired in BONJ-related mucoperiosteal tissue, but vascularization remains unaffected. Vessel remodeling and neovessel formation is delayed in BONJ, resulting in impaired tissue regeneration of bisphosphonate-exposed oral mucosa.
Author List
Wehrhan F, Stockmann P, Nkenke E, Schlegel KA, Guentsch A, Wehrhan T, Neukam FW, Amann KAuthor
Arndt Geuntsch in the CTSI department at Medical College of Wisconsin - CTSIMESH terms used to index this publication - Major topics in bold
Alkaline PhosphataseAlveolar Process
Antigens, CD
Bone Density Conservation Agents
Capillaries
Coloring Agents
Connective Tissue
Diphosphonates
Endoglin
Endothelial Cells
Endothelium, Vascular
Humans
Image Processing, Computer-Assisted
Imidazoles
Immunohistochemistry
Jaw Diseases
Mouth Mucosa
Neovascularization, Physiologic
Osteonecrosis
Periosteum
Platelet Endothelial Cell Adhesion Molecule-1
Receptors, Cell Surface
