Effects of cholecystokinin and hydrocortisone on DNA and protein synthesis in immature rat pancreas. Pancreas 1988;3(3):274-8
Date
01/01/1988Pubmed ID
3387421DOI
10.1097/00006676-198805000-00006Scopus ID
2-s2.0-0024261742 (requires institutional sign-in at Scopus site) 7 CitationsAbstract
To define the developmental pattern of the trophic effects of cholecystokinin octapeptide (CCK-8) and hydrocortisone on immature rat pancreas, we injected newborn rats, rats aged 4, 7, 11, 18, and 25 days and 3 months, and adult rats with CCK (5 and 10 micrograms/kg) in gelatin and hydrocortisone (10 mg/kg) for 3 days. Animals were killed, the pancreata were removed, and the concentrations of DNA and protein were measured and DNA and protein synthesis rates determined by incorporation of [3H]thymidine and [14C]leucine, respectively. These values were compared with those of saline-injected controls. DNA concentration was significantly increased over control at ages 2 days to adult by hydrocortisone and by CCK (10 micrograms/kg) in the adult. Protein concentration was increased on days 3-14 by hydrocortisone. DNA synthesis was increased by CCK and decreased by hydrocortisone at 3 months and adult. Protein synthesis was decreased by hydrocortisone at ages 3-14 days. Thus, each agent has its own developmental pattern with age on the rat pancreas.
Author List
Werlin SL, Virojanavat S, Reynolds E, Hoffman RG, Colton DGAuthor
Steven L. Werlin MD Emeritus Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Age FactorsAnimals
Cholecystokinin
DNA
Hydrocortisone
Male
Pancreas
Protein Biosynthesis
Rats
Rats, Inbred Strains
