Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish. PLoS One 2012;7(3):e31658
Date
03/23/2012Pubmed ID
22438865Pubmed Central ID
PMC3306315DOI
10.1371/journal.pone.0031658Scopus ID
2-s2.0-84863374423 (requires institutional sign-in at Scopus site) 24 CitationsAbstract
The transcription factor etsrp/Er71/Etv2 is a master control gene for vasculogenesis in all species studied to date. It is also required for hematopoiesis in zebrafish and mice. Several novel genes expressed in vasculature have been identified through transcriptional profiling of zebrafish embryos overexpressing etsrp by microarrays. Here we re-examined this transcriptional profile by Illumina RNA-sequencing technology, revealing a substantially increased number of candidate genes regulated by etsrp. Expression studies of 50 selected candidate genes from this dataset resulted in the identification of 39 new genes that are expressed in vascular cells. Regulation of these genes by etsrp was confirmed by their ectopic induction in etsrp overexpressing and decreased expression in etsrp deficient embryos. Our studies demonstrate the effectiveness of the RNA-sequencing technology to identify biologically relevant genes in zebrfish and produced a comprehensive profile of genes previously unexplored in vascular endothelial cell biology.
Author List
Gomez G, Lee JH, Veldman MB, Lu J, Xiao X, Lin SAuthor
Matthew B. Veldman PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAnimals, Genetically Modified
Base Sequence
Blood Vessels
Gene Expression Profiling
Gene Expression Regulation, Developmental
Hematopoiesis
High-Throughput Nucleotide Sequencing
Mice
Oligonucleotide Array Sequence Analysis
RNA
Zebrafish
Zebrafish Proteins