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Phospholipase Cgamma2 provides survival signals via Bcl2 and A1 in different subpopulations of B cells. J Biol Chem 2003 Oct 31;278(44):43654-62



Pubmed ID




Scopus ID

2-s2.0-0242321892   36 Citations


PLCgamma2 plays a critical role in B cell receptor (BCR) signaling and its targeted deletion results in defective B cell development and function. Here, we show that PLCgamma2 deficiency specifically blocks B cell maturation at the transitional type 2 (T2) to follicular (FO) B cell transition and the PLCgamma2 pathway regulates survival of B cells. BCR-induced apoptosis is dramatically enhanced in all subsets of splenic PLCgamma2-deficient B cells, especially in T2 and FO B cell subpopulations. We also find that all splenic PLCgamma2-deficient B cell subpopulations express abnormally low levels of Bcl-2 protein. In addition, PLCgamma2 deficiency disrupts BCR-mediated induction of A1 expression. Enforced expression of Bcl-2 prevents BCR-induced apoptosis in all splenic PLCgamma2-deficient B cell subpopulations and partially restores the numbers of PLCgamma2-deficient FO B cells. In contrast to Bcl-2, enforced expression of A1 preferentially prevents BCR-induced apoptosis in PLCgamma2-deficient FO B cells and partially restores the numbers of these B cells. Therefore, the PLCgamma2 pathway provides a survival signal via regulation of Bcl-2 in all splenic B cell subpopulations and via additional induction of A1 in mature FO B cells.

Author List

Wen R, Chen Y, Xue L, Schuman J, Yang S, Morris SW, Wang D


Demin Wang PhD Assistant Professor in the Microbiology and Immunology department at Medical College of Wisconsin
Renren Wen PhD Associate Investigator in the Blood Research Institute department at BloodCenter of Wisconsin

MESH terms used to index this publication - Major topics in bold

Blotting, Western
Bone Marrow Transplantation
Cell Separation
Cell Survival
Cells, Cultured
DNA-Binding Proteins
Flow Cytometry
In Situ Nick-End Labeling
Phospholipase C gamma
Proto-Oncogene Proteins c-bcl-2
Replication Protein C
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Time Factors
Type C Phospholipases
jenkins-FCD Prod-469 c3fc8ab87196149f9b23743c01b947d47e7319e5