Faculty Collaboration Database

Complementation and characterization of chemotaxis mutants of Bacillus subtilis. J Bacteriol 1985 Nov;164(2):802-10

Date

11/01/1985

Pubmed ID

3932330

Pubmed Central ID

PMC214322

DOI

10.1128/JB.164.2.802-810.1985

Scopus ID

2-s2.0-0022345182   17 Citations

Abstract

A set of chemotaxis mutants of Bacillus subtilis was complemented by using SP beta c2 transducing bacteriophage either containing cloned segments of DNA or derived from abnormal excision of SP beta c2 dl2::Tn917 inserted into the chemotaxis region. Representative mutants were characterized in capillary assays for chemotaxis toward four amino acids and mannitol and in tethered-cell experiments for addition and removal of two attractants and two repellents. Twenty complementation groups were identified, in addition to the cheR previously characterized. All were found to be defective in chemotaxis toward all chemoeffectors. They were assigned the names cheA through cheU. The large number of general chemotaxis genes in B. subtilis, in contrast to the six in Escherichia coli, suggests fundamental differences in the mechanism of chemotaxis in the two species.

Author List

Ordal GW, Parker HM, Kirby JR

Author

John Kirby PhD Chair, Center Associate Director, Professor in the Microbiology and Immunology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Amino Acids
Bacillus subtilis
Chemotaxis
Genes, Bacterial
Genetic Complementation Test
Mannitol
Mutation