Faculty Collaboration Database

Mature IgD^low/- B cells maintain tolerance by promoting regulatory T cell homeostasis. Nat Commun 2019 Jan 14;10(1):190

Date

01/16/2019

Pubmed ID

30643147

Pubmed Central ID

PMC6331566

DOI

10.1038/s41467-018-08122-9

Scopus ID

2-s2.0-85060004031 (requires institutional sign-in at Scopus site)   24 Citations

Abstract

A number of different B cell subsets have been shown to exhibit regulatory activity using a variety of mechanisms to attenuate inflammatory diseases. Here we show, using anti-CD20-mediated partial B cell depletion in mice, that a population of mature B cells distinguishable by IgD^low/- expression maintains tolerance by, at least in part, promoting CD4⁺Foxp3⁺ regulatory T cell homeostatic expansion via glucocorticoid-induced tumor necrosis factor receptor ligand, or GITRL. Cell surface phenotyping, transcriptome analysis and developmental study data show that B cells expressing IgD at a low level (BD_L) are a novel population of mature B cells that emerge in the spleen from the transitional-2 stage paralleling the differentiation of follicular B cells. The cell surface phenotype and regulatory function of BD_L are highly suggestive that they are a new B cell subset. Human splenic and peripheral blood IgD^low/- B cells also exhibit BD_L regulatory activity, rendering them of therapeutic interest.

Author List

Ray A, Khalil MI, Pulakanti KL, Burns RT, Gurski CJ, Basu S, Wang D, Rao S, Dittel BN

Authors

Sridhar Rao MD, PhD Associate Professor in the Pediatrics department at Medical College of Wisconsin
Demin Wang PhD Professor in the Microbiology and Immunology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
B-Lymphocyte Subsets
Cell Separation
Cells, Cultured
Coculture Techniques
Dermatitis, Contact
Disease Models, Animal
Flow Cytometry
Gene Expression Profiling
Gene Expression Regulation, Developmental
Healthy Volunteers
Humans
Immune Tolerance
Immunoglobulin D
Leukocytes, Mononuclear
Mice
Mice, Inbred C57BL
Oxazolone
Spleen
T-Lymphocytes, Regulatory
Tumor Necrosis Factors