Use of transgenic and knockout strategies in mice. Semin Nephrol 2002 Mar;22(2):154-60
Date
03/14/2002Pubmed ID
11891509DOI
10.1053/snep.2002.30220Scopus ID
2-s2.0-0036124346 (requires institutional sign-in at Scopus site) 3 CitationsAbstract
Over 2 decades ago mouse models were generated with an exogenous gene integrated into its genome to create the first transgenic mice. Since that time, new methods have been developed and old methods improved, allowing investigators more flexibility to answer important questions about physiology and gene function. Transgenic and knockout technology have been particularly useful in the kidney as various transgenic mouse models have been successfully generated to gain a better understanding of renal physiology at the gene level. Now with the sequencing of mammalian genomes at the forefront of science, the need for transgenic technology to understand gene function in the context of the whole animal will become increasingly more important. Therefore, this article focuses on some of the strategies that can be used when generating transgenic mouse models.
Author List
Ryan MJ, Sigmund CDAuthor
Curt Sigmund PhD Chair, Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsDisease Models, Animal
Gene Expression
Gene Transfer Techniques
Genetic Engineering
Integrases
Kidney
Mice
Mice, Knockout
Mice, Transgenic
Transgenes
Viral Proteins