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Protein composition of plasminogen activator inhibitor type 1-derived endothelial microparticles. Shock 2008 Apr;29(4):504-11

Date

07/04/2008

Pubmed ID

18598005

DOI

10.1097/shk.0b013e3181454898

Scopus ID

2-s2.0-40949144531   22 Citations

Abstract

Endothelial microparticles (EMPs) are small vesicles released from the plasma membrane of endothelial cells in response to cell injury, apoptosis, or activation. Low levels of MPs are shed into the blood from the endothelium, but in some pathologic states, the number of EMPs is elevated. The mechanism of MP formation and the wide-ranging effects of elevated EMPs are poorly understood. Here, we report the protein composition of EMPs derived from human umbilical cord endothelial cells stimulated with plasminogen activator inhibitor type 1 (PAI-1). Two-dimensional gel electrophoresis followed by mass spectrometry identified 58 proteins, of which some were verified by Western blot analysis. Gene Ontology database searches revealed that proteins identified on PAI-1-derived EMPs are highly diverse. Endothelial microparticles are composed of proteins from different cellular components that exhibit multiple molecular functions and are involved in a variety of biological processes. Important insight is provided into the generation and protein composition of PAI-1-derived EMPs.

Author List

Sander TL, Ou JS, Densmore JC, Kaul S, Matus I, Twigger S, Halligan B, Greene AS, Pritchard KA Jr, Oldham KT

Authors

John C. Densmore MD Professor in the Surgery department at Medical College of Wisconsin
Keith T. Oldham MD Professor in the Surgery department at Medical College of Wisconsin
Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Blotting, Western
Cell Line
Electrophoresis, Gel, Two-Dimensional
Endothelial Cells
Humans
Isoelectric Focusing
Plasminogen Activator Inhibitor 1
Proteins
Proteomics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a