Faculty Collaboration Database

Intestinal adaptation for oligopeptide absorption via PepT1 after massive (70%) mid-small bowel resection. J Gastrointest Surg 2011 Feb;15(2):240-7; discussion 247-9

Date

12/21/2010

Pubmed ID

21170601

Pubmed Central ID

PMC3050655

DOI

10.1007/s11605-010-1320-x

Scopus ID

2-s2.0-79751525253 (requires institutional sign-in at Scopus site)   13 Citations

Abstract

INTRODUCTION: Proteins are absorbed primarily as short peptides via peptide transporter 1 (PepT1).

HYPOTHESIS: Intestinal adaptation for peptide absorption after massive mid-small intestinal resection occurs by increased expression of PepT1 in the remnant small intestine and colon.

METHODS: Peptide uptake was measured in duodenum, jejunum, ileum, and colon using glycyl-sarcosine 1 week (n = 9) and 4 weeks (n = 11) after 70% mid-small bowel resection and in corresponding segments from unoperated rats (n = 12) and after transection and reanastomosis of jejunum and ileum (n = 8). Expression of PepT1 (mRNA, protein) and villus height were measured.

RESULTS: Intestinal transection/reanastomosis did not alter gene expression. Compared to non-operated controls, 70% mid-small bowel resection increased jejunal peptide uptake (p < 0.05) associated with increased villus height (1.13 vs 1.77 and 1.50 mm, respectively, p < 0.01). In ileum although villus height increased at 1 and 4 weeks (1.03 vs 1.21 and 1.35 mm, respectively; p < 0.01), peptide uptake was not altered. PepT1 mRNA and protein were decreased at 1 week, and PepT1 protein continued low at 4 weeks. Gene expression, peptide uptake, and histomorphology were unchanged in the colon.

CONCLUSIONS: Jejunal adaptation for peptide absorption occurs by hyperplasia. Distal ileum and colon do not have a substantive role in adaptation for peptide absorption.

Author List

Madhavan S, Scow JS, Chaudhry RM, Nagao M, Zheng Y, Duenes JA, Sarr MG

Author

Srivats Madhavan MBBS Associate Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adaptation, Physiological
Animals
Body Weight
Colon
Duodenum
Ileum
Intestinal Absorption
Intestinal Mucosa
Jejunum
Male
Peptide Transporter 1
Peptides
RNA, Messenger
Rats
Rats, Inbred Lew
Statistics, Nonparametric
Symporters