Neurospora glucamylase and a mutant affected in its regulation. Biochem Genet 1985 Feb;23(1-2):89-103
Date
02/01/1985Pubmed ID
3922351DOI
10.1007/BF00499115Scopus ID
2-s2.0-0021914096 (requires institutional sign-in at Scopus site) 16 CitationsAbstract
Neurospora glucamylase is a glucose-repressible extracellular enzyme. The enzyme was purified to homogeneity and found to have a molecular weight of 82,000 and to release glucose from either maltose or amylose. The rate of glucamylase synthesis increases more than 100-fold when cells are transferred from a glucose-containing medium to a glucose-free medium. Increased from a glucose-containing medium to a glucose-free medium. Increased production of glucamylase begins within 30 min of the transfer. Glucamylase is rapidly secreted into the medium. A mutant affecting the ability of glucose to repress the synthesis of the glucose-repressible extracellular enzymes glucamylase and invertase has been isolated and studied. The mutant constitutively synthesizes and secretes a glucamylase which is indistinguishable from the wild-type enzyme.
Author List
Sigmund RD, McNally MT, Lee DB, Free SJAuthor
Mark T. McNally PhD Associate Professor in the Microbiology and Immunology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Electrophoresis, Polyacrylamide GelGene Expression Regulation
Genes, Fungal
Glucan 1,4-alpha-Glucosidase
Glucosidases
Mutation
Neurospora