Three dynamin-encoding genes are differentially expressed in developing rat brain. J Neurochem 1996 Sep;67(3):927-31
Date
09/01/1996Pubmed ID
8752097DOI
10.1046/j.1471-4159.1996.67030927.xScopus ID
2-s2.0-0029799506 (requires institutional sign-in at Scopus site) 81 CitationsAbstract
Dynamin proteins are members of a recently described family of GTPases involved in receptor-mediated processes. To date, three different dynamin-encoding genes have been identified in mammalian tissues. Dynamin I is expressed only in neurons, whereas dynamin II is ubiquitously expressed. A third isoform, dynamin III, was originally isolated from a rat testis cDNA library and shown to be testis-specific. However, here we report the cloning and characterization of dynamin III from brain and lung, demonstrating a more extended pattern of expression for this isoform. In addition, we have investigated the temporal pattern of expression of these three genes during brain development. We find that both dynamin I and dynamin III mRNA levels are up-regulated during embryogenesis, whereas dynamin II mRNA levels remain unchanged. From these results, we conclude that dynamin III is not a testis-specific isoform and, furthermore, that rat brain expresses three different dynamin-encoding genes that are differentially regulated during development. Therefore, this large isoform diversity of dynamin proteins in brain predicts a significant complexity in the understanding of dynamin-based processes in this tissue.
Author List
Cook T, Mesa K, Urrutia RAuthor
Raul A. Urrutia MD Center Director, Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Alternative SplicingAmino Acid Sequence
Animals
Brain
Dynamin I
Dynamin III
Dynamins
Endocytosis
GTP Phosphohydrolases
Gene Expression Regulation, Developmental
Gene Library
Genetic Testing
Isoenzymes
Lung
Male
Microtubules
Molecular Sequence Data
PC12 Cells
RNA, Messenger
Rats
Rats, Sprague-Dawley
Testis
Time Factors
