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Mesh term Oligonucleotide Probes

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Nucleic Acid Probes

Description

Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.



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jenkins-FCD Prod-468 69a93cef3257f26b866d455c1d2b2d0f28382f14