CURRICULUM VITAE
Jacek M. Zielonka PhD
Jacek M. Zielonka PhD
Assistant Professor
Department of Biophysics
Department of Biophysics
OFFICE ADDRESS: |
Translational and Biomedical Research Center |
8701 Watertown Plank Rd |
Milwaukee, WI 53226 |
EDUCATION: |
10/01/1993 - 09/17/1998 M.Sc., Technical University of Lodz, Lodz, Poland |
10/01/1998 - 09/15/2002 Ph.D., Technical University of Lodz, Lodz, Poland |
2015 D.Sc., Chemistry Sciences, Lodz University of Technology, Lodz, Poland |
POSTGRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS: |
04/01/2004 - 02/28/2007 Postdoctoral Fellow, Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226 |
FACULTY APPOINTMENTS: |
07/01/2018 - Present Assistant Professor, Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226 |
RESEARCH ADMINISTRATIVE APPOINTMENTS: |
03/01/2007 - 01/31/2011 Research Scientist I, Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226 |
09/01/2010 - 08/31/2015 Project Consultant, Institute of Applied Radiation Chemistry, Lodz University of Technology, Zeromskiego 116, Lodz, Poland |
02/01/2011 - 06/30/2015 Facility Manager, Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 |
02/01/2011 - 06/30/2015 Research Scientist II, Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226 |
07/01/2015 - 06/30/2018 Research Director, Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226 |
AWARDS AND HONORS: |
2004 Award for the co-authorship of the series of papers on the photochemical and radiolytic generation of unstable chemical species of significant cognitive importance., the Polish Radiation Research Society |
2004 Best Teaching Assistant, Student Association of the Faculty of Chemistry, Lodz University of Technology, Poland |
2004 Award for the co-authorship of the series of papers concerning the discovery of the therapeutic function of selected analogs of NAD coenzyme., Polish Ministry of Education and Sport |
2012 Top Reviewer Award , journal Free Radical Biology & Medicine |
2018 Outstanding Reviewer Award, journal Free Radical Biology & Medicine |
2019 First degree team award for co-authorship of the paper, "Mechanism of oxidative conversion of Amplex® Red to resorufin: Pulse radiolysis and enzymatic studies", the Polish Radiation Research Society |
MEMBERSHIPS IN HONORARY AND PROFESSIONAL SOCIETIES: |
10/2008 - Present Society for Redox Biology and Medicine (Member) |
03/2013 - Present American Association for Cancer Research (Member) |
05/2014 - Present American Chemical Society (Member) |
EDITORSHIPS/EDITORIAL BOARDS/JOURNAL REVIEWS: |
Editorial Board |
01/2012 - 01/2020 Free Radical Biology & Medicine |
12/2014 - 01/2019 Oxidative Medicine and Cellular Longevity |
02/2016 - 01/2020 Reactive Oxygen Species |
Journal Review |
2007 - Present Free Radical Biology & Medicine |
Ad-Hoc Reviewer |
2005 - Present Journal of Physical Chemistry |
06/2006 - Present Biochimica & Biophysica Acta |
11/2007 - Present Nature Protocols |
06/2008 - Present American Journal of Physiology - Heart and Circulatory Physiology |
03/2009 - Present Journal of Photochemistry and Photobiology |
04/2009 - Present Redox Reports |
08/2009 - Present Radiation Research |
02/2010 - Present Journal of Applied Physiology |
03/2012 - Present Free Radical Research |
07/2012 - Present Nitric Oxide |
09/2012 - Present FEBS Letters |
11/2012 - Present PLoS One |
04/2013 - Present Sensors and Actuators B: Chemical |
12/2013 - Present Radiation Physics and Chemistry |
01/2014 - Present Chemical Science |
07/2014 - Present Nature Communications |
08/2014 - Present Chemical Research in Toxicology |
08/2014 - Present Laboratory Investigation |
12/2014 - Present Oxidative Medicine and Cellular Longevity |
12/2014 - Present Present American Journal of Physiology – Regulatory, Integrative and Comparative Physiology |
04/2015 - Present Acta Biochimica Polonica |
07/2015 - Present Analytical Biochemistry: Methods in the Biological Sciences |
04/2016 - Present Methods |
04/2016 - Present British Journal of Pharmacology |
04/2016 - Present Biomaterials |
11/2016 - Present Interface Focus |
11/2016 - Present Molecules |
12/2016 - Present Archives of Biochemistry and Biophysics |
04/2017 - Present Expert Opinion on Drug Discovery |
09/2017 - Present Chemosensors |
09/2017 - Present Scientific Reports |
06/2018 - Present Biological Chemistry |
12/2018 - Present Journal of Environmental Management |
02/2019 - Present Cancers |
09/2019 - Present ACS Chemical Biology |
10/2019 - Present Redox Biology |
11/2019 - Present Cell Biology and Toxicology |
12/2019 - Present RSC Medicinal Chemistry |
12/2019 - Present Biomolecules |
02/2020 - Present Journal of Translational Genetics and Genomics |
03/2020 - Present Dyes & Pigments |
03/2020 - Present Chemical Communications |
04/2020 - Present ACS Omega |
05/2020 - Present Journal of the American Chemical Society |
05/2020 - Present MedComm |
06/2020 - Present Spectrochimica Acta |
06/2020 - Present Antioxidants |
08/2020 - Present Critical Reviews in Biochemistry & Molecular Biology |
08/2020 - Present ACS Sensors |
NATIONAL ELECTED/APPOINTED LEADERSHIP AND COMMITTEE POSITIONS: |
- 11/2010 Reviewer of the oral presentations and posters for the Young Investigator Award competition, 8. Society for Free Radical Biology and Medicine 17th Annual Meeting, Orlando, FL, USA |
- 11/2016 Workshop Co-chair, Society for Free Radical Biology and Medicine 23rd Annual Meeting, Fundamentals in Redox Biology pre-meeting workshop, San Francisco, CA, USA |
- 02/2014 Session Chair, Oxygen Radicals Gordon Conference, Ventura, CA, USA |
- 02/2016 Session Co-chair, Oxygen Radicals Gordon Conference, Ventura, CA, USA |
INTERNATIONAL ELECTED/APPOINTED LEADERSHIP AND COMMITTEE POSITIONS: |
06/2012 Session Chair, IARC Workshop on Reactive Intermediates for Graduate Students and Young Scholars, Spała, Poland |
04/2016 Session Chair, ROS in Drug Discovery: From Target Identification to Redox-Based Therapeutics, COST Action BM1203 Working Groups Meeting, Lisbon, Portugal |
10/2016 Session Co-Chair, X International Workshop on EPR in Biology and Medicine, Kraków, Poland |
09/2017 Session Co-Chair, Second Adriatic Symposium on Biophysical Approaches in Biomedical Studies, Split, Croatia |
10/2019 Session Co-Chair, XI International Workshop on EPR in Biology and Medicine, Kraków, Poland |
RESEARCH GRANTS/AWARDS/CONTRACTS/PROJECTS: | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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INVITED LECTURES/WORKSHOPS/PRESENTATIONS: |
Local |
Zielonka J., Zielonka M., VerPlank L., Ganesh T., Sun A., Cheng G., Communal C., O'Brien W., Lambeth D., Kalyanaraman B., Targeting NADPH oxidases in cardiovascular diseases: Development of assays for high throughput screening of Nox2 inhibitors, Cardiovascular Redox Signaling Symposium, Milwaukee, WI, USA, 11/2014 |
Zielonka J., Foolproof detection of superoxide - Are we there yet?, Biophysics Graduate Seminar Series, Medical College of Wisconsin, Milwaukee, WI, USA, 03/2018 |
Zielonka J., Development of a specific assay for peroxynitrite detection, Second MCW Redox Biology Symposium, Milwaukee, WI, USA, 04/2018 |
National |
Zielonka J., Measuring Cellular Oxidizing Species with Small Molecule Luminescent Probes, Society for Free Radical Biology and Medicine 21st Annual Meeting (pre-meeting workshop), Seattle, WA, USA, 11/2014 |
Zielonka J.,, Superoxide and Hydrogen Peroxide Detection in Biological systems: From Theory to Practice, NADPH Oxidases Gordon Seminar, Waterville Valley Resort, Waterville Valley, NH, USA, 06/2016 |
Zielonka J., Zielonka M., Cheng G., Lambeth D., Kalyanaraman B., Development of new assays for high-throughput screening of NADPH oxidase inhibitors, NADPH Oxidases Gordon Conference, Waterville Valley Resort, Waterville Valley, NH, USA, 06/2016 |
Zielonka J., Measurement of Cellular Oxidants with Small Molecule Probes, Society for Free Radical Biology and Medicine 23rd Annual Meeting (pre-meeting workshop), San Francisco, CA, USA, 11/2016 |
Zielonka J.,, Recent Advances in Specific Detection of Superoxide, Oxygen Radicals Gordon Conference, Ventura, CA, USA, 02/2018 |
Zielonka J., Detection of ONOO- by boronates - selectivity, specificity and mechanism, Nitric Oxide Gordon Conference, Ventura, CA, USA, 02/2019 |
International |
Zielonka J., Sarna T., Kalyanaraman B., Superoxide reaction with hydroethidine and mito-hydroethidine. EPR, UV-Vis absorption, fluorescence and HPLC studies, VII International Workshop on EPR in Biology and Medicine, Kraków, Poland, 10/2007 |
Zielonka J., Sikora A., Lopez M., Hardy M., Zielonka M., Joseph J., Kalyanaraman B., Detection of peroxynitrite by boronate-based probes: EPR, fluorescence and HPLC studies, EPR 2010 Conference, San Juan, Puerto Rico, 05/2010 |
Zielonka J., Sikora A., Joseph J., Hardy M., Ouari O., Adamus J., Kalyanaraman B., Detection of peroxynitrite and its precursors, nitric oxide and superoxide radical anion in cell-free and cellular systems, VIII International Workshop on EPR in Biology and Medicine, Kraków, Poland, 10/2010 |
Zielonka J., Kielar F., Zielonka M., Sikora A., Franz K.J., Kalyanaraman B., Kinetic aspects of activation of boronate-based iron prochelators in cell-free and cellular systems, IARC Workshop on Reactive Intermediates for Graduate Students and Young Scholars, Spała, Poland, 06/2012 |
Zielonka J., Sikora A., Michalski R., Hardy M., Dranka B., Joseph J., Adamus J., Kalyanaraman B., New methods of detection of reactive oxygen and nitrogen species: from mechanistic studies to biological applications, The 6th European Young Investigator Conference, Słubice, Poland, 06/2013 |
Zielonka J., Cheng G., Zielonka M., Ganesh T., Sun A., Joseph J., Michalski R., O'Brien W., Lambeth D., Kalyanaraman B., High throughput screening of inhibitors of NADPH oxidases (Nox) - a combination of fluorescence, HPLC and EPR assays for reliable identification of novel Nox inhibitors, IX International Workshop on EPR in Biology and Medicine, Kraków, Poland, 10/2013 |
Zielonka J., Free radical metabolism and bioenergetic changes in cardiovascular diseases and cancer, First Adriatic Symposium on Biophysical Approaches in Biomedical Studies, Split, Croatia, 08/2014 |
Zielonka J., Targeting NADPH oxidases: New assays for high throughput screening of Nox2 inhibitors, First International Munich ROS Meeting of COST Action BM1203, Munich, Germany, 04/2015 |
Zielonka J., Fluorescence spin trapping - a new method for sensitive detection of superoxide, ROS in drug discovery: from target identification to redox-based therapeutics, COST Action BM1203 Working Groups Meeting, Lisbon, Portugal, 04/2016 |
Zielonka J., Novel Inhibitors of NADPH Oxidase (Nox) enzymes, X International Workshop on EPR in Biology and Medicine, Kraków, Poland, 10/2016 |
Zielonka J., Sikora A., Hardy M., Pięta J., Cheng G., Zielonka M., Kalyanaraman B., Modulation of mitochondrial redox status as a new strategy to selectively block the proliferation of cancer cells, Signaling Molecules and Biological Oxidants seminar, Konopnica, Poland, 09/2018 |
Zielonka J., Sikora A., Hardy M., Podsiadły R., Michalski R., Zielonka M., Kalyanaraman B, Kinetic aspects of the detection of reactive oxygen and nitrogen species in cellular and cell-free systems, 9th PULS Conference on Pulse Investigations in Chemistry, Physics and Biology, Lodz, Poland, 09/2018 |
Stauder E., Zielonka M., Sikora A., Hardy M., Kalyanaraman B., Zielonka J., Mitochondria-targeted pyridinium cations as redox cyclers mitochondrial inhibitors and antiproliferative agents against pancreatic cancer cells, XI International Workshop on EPR in Biology and Medicine, Kraków, Poland, 10/2019 |
PEER REVIEWED WORKSHOPS/PRESENTATIONS: |
Regional |
Zielonka J., Sikora A., Pięta J., Zielonka M., Cheng G., Kalyanaraman B., Targeting redox-active pyridinium cations to mitochondria to inhibit proliferation of colon cancer cells (poster), Society for Free Radical Biology and Medicine 25th Annual Meeting, Chicago, IL, USA, 11/2018 |
National |
Zielonka J., Sarna T., Roberts J.E., Wishart J.F., Kalyanaraman B., The reaction of superoxide with hydroethidine and its mitochondria-targeted analog: pulse radiolysis and steady-state analyses (poster), Society for Free Radical Biology and Medicine 13th Annual Meeting, Denver, CO, USA, 11/2006 |
Zielonka J., Sikora A., Zielonka M., Joseph J., Hardy M., Kalyanaraman B., Monitoring of peroxynitrite release from activated macrophages: The use of novel boronate-based fluorogenic probes (poster), Society for Free Radical Biology and Medicine 16th Annual Meeting, San Francisco, CA, USA, 11/2009 |
Zielonka J., Sikora A., Lopez M., Joseph J., Kalyanaraman B., Mechanistic analysis of the reaction of ONOO- with boronate-based probes (poster), Oxygen Radicals Gordon Conference, Ventura, CA, 02/2010 |
Zielonka J., Zielonka M., Sikora A., Adamus J., Joseph J., Hardy M., Ouari O., Kalyanaraman B., Global profiling of reactive oxygen and nitrogen species in cell-free and model cellular systems – real time fluorescence monitoring and HPLC measurements (poster), Society for Free Radical Biology and Medicine 17th Annual Meeting, Orlando, FL, USA, 11/2010 |
Zielonka J., Dranka B.P., Zielonka M., Joseph J., Hardy M., Sikora A., Kalyanaraman B., Reduction of mitochondrial reserve capacity in endothelial cells by nitric oxide and superoxide – detection and quantitation of peroxynitrite formed from co-generated •NO and O2•- (poster), Society for Free Radical Biology and Medicine 17th Annual Meeting, Orlando, FL, USA, 11/2010 |
Zielonka J., Zielonka M., Ganesh T., Sun A., Lambeth D., Kalyanaraman B., Inhibitors of NADPH oxidase decrease peroxynitrite formation in activated macrophages (poster), Society for Free Radical Biology and Medicine 18th Annual Meeting, Atlanta, GA, USA, 11/2011 |
Zielonka J., Kielar F., Sikora A., Franz K.J., Kalyanaraman B., Dual antioxidant activity of boronate-based prochelators: scavenging peroxynitrite and metal ion chelation (poster), Society for Free Radical Biology and Medicine 18th Annual Meeting, Atlanta, GA, USA, 11/2011 |
Zielonka J., Kielar F., Zielonka M., Sikora A., Franz K.J., Kalyanaraman B., Kinetic aspects of activation of boronate-based iron prochelators in cell-free and cellular systems (poster), Oxygen Radicals Gordon Conference, Ventura, CA, USA, 02/2012 |
Zielonka J., Zielonka M., Ganesh T., Sun A., Joseph J., Cheng G., O’Brien W., Lambeth D., Kalyanaraman B., Development of new assays for high-throughput screening of NADPH oxidase inhibitors (selected oral presentation), NADPH Oxidases Gordon Conference, Waterville Valley Resort, Waterville Valley, NH, USA, 06/2012 |
Zielonka J., Zielonka M., Ganesh T., Sun A., Joseph J., Cheng G., O’Brien W., Lambeth D., Kalyanaraman B., In search for inhibitors of NADPH oxidases: New assays for superoxide and hydrogen peroxide as a platform for high throughput screening (HTS) of inhibitors of NADPH oxidases (poster), Society for Free Radical Biology and Medicine 19th Annual Meeting, San Diego, CA, USA, 11/2012 |
Zielonka J., Cheng G., McAllister D.M., Craig Mackinnon Jr. A., Joseph J., Dwinell M.B., Kalyanaraman B., Mitochondria-targeted chromanol inhibits breast cancer cell energy metabolism and promotes cell death (poster), Target Cancer Metabolism, Boston, MA, USA, 01/2013 |
Cheng G., Zielonka J., Joseph J., Kalyanaraman B., Bioenergetic changes in glycolysis, Krebs cycle, and glutaminolysis in pancreatic cells treated with mitochondria-targeted cationic drugs (poster), American Association for Cancer Research Annual Meeting, Washington D.C., USA, 04/2013 |
Zielonka J., Lambeth D., Kalyanaraman B., On the chemiluminescent probes for superoxide: Is L-012 probe a reliable tool for detection and quantification of O2•–? (poster), Society for Free Radical Biology and Medicine 20th Annual Meeting, San Antonio, TX, USA, 11/2013 |
Zielonka J., VerPlank L., Zielonka M., Cheng G., Communal C., Kalyanaraman B., In search for new inhibitors of NADPH oxidases: high throughput screening of bioactive compounds libraries (poster), Oxygen Radicals Gordon Conference, Ventura, CA, USA, 02/2014 |
Zielonka J., Spin trapping with fluorescence detection: Novel method for specific and sensitive fluorimetric detection of superoxide radical anion (selected oral presentation), Society for Free Radical Biology and Medicine 22nd Annual Meeting, Boston, MA, USA, 11/2015 |
Zielonka J., Podsiadły R., Hardy M., Kalyanaraman B., Bioluminescent detection of cellular oxidants using Peroxy-Caged Luciferin-1 (PCL-1): Identification of reaction intermediates and oxidant-specific minor products (poster), Oxygen Radicals Gordon Conference, Ventura, CA, USA, 02/2016 |
Zielonka J., Rios N., Radi R., Kalyanaraman B., Following oxygen atoms during oxidation of boronate probes by hydrogen peroxide and peroxynitrite – isotope labeling study (poster), Nitric Oxide Gordon Conference, Ventura, CA, USA, 02/2019 |
Stauder E., Juric M., Sikora A., Hardy M., Zielonka M., Kalyanaraman B., Zielonka J., Simultaneous induction of bioenergetic and redox stress as a strategy to inhibit proliferation and induce death of pancreatic cancer cells (poster), Society for Free Radical Biology and Medicine 26th Annual Meeting, Las Vegas, NV, USA, 11/2019 |
Juric M., Sikora A., Pieta J., Hardy M., Zielonka M., Kalyanaraman B., Zielonka J., Novel mitochondria-targeted redox cycling agent based on nitropyridinium cation (poster), Oxygen Radicals Gordon Conference, Ventura, CA, USA, 02/2020 |
International |
Zielonka J., Adamus J., Marcinek A., Gębicki J., Reactive Intermediates Generated by One-electron Oxidation of NADH (poster), International Workshop on Reactive Intermediates IWRI'99, Szczyrk, Poland, 08/1999 |
Zielonka J., Marcinek A., Gębicki J., Gordon C.M., Dunkin I.R., Ionic Liquids as a Novel Glassy Medium for Direct Characterization of Radical Ions (poster), The Chemistry and Physics of Matrix Isolated Species, Szklarska Poręba, Poland, 07/2001 |
Zielonka J., Gębicki J., Molecular mechanisms of pyridinium salts cytotoxicity: reactivity of pyridinyl radicals against oxygen (poster), NATO Advanced Study Institute on Free Radicals, Nitric Oxide, and Inflammation: Molecular, Biochemical, and Clinical Aspects, Antalya, Turkey, 09/2001 |
Zielonka J., Gębicki J., Pyridinyl radicals as electron donors (poster), International Conference on Reactive Intermediates and Reaction Mechanisms, Ascona, Switzerland, 07/2002 |
Zielonka J., Adamus J., Marcinek A., Gębicki J., Reactive Intermediates Generated by One-electron Oxidation of NADH (poster), EPA Graduate Student Symposium in Photochemistry, Fribourg, Switzerland, 02/2010 |
Zielonka J., Marcinek A., Gębicki J., Gordon C.M., Dunkin I.R., Generation of Radical Cations in Frozen Ionic Liquids (poster), EPA Graduate Student Symposium in Photochemistry, Fribourg, Switzerland, 02/2010 |
Zielonka J., Adamus J., Marcinek A, Gębicki J., Photochemically Induced Dehydration of 1-Benzyl-3-acetyl-6-hydroxy-1,4,5,6-tetrahydropyridine (poster), EPA Graduate Student Symposium in Photochemistry, Fribourg, Switzerland, 02/2010 |
MEDICAL COLLEGE TEACHING ACTIVITIES: |
Graduate Student Education |
02/2018 Taught “Fundamentals of Bioenergetics in Cancer Metabolism” within the course Current Concepts of Cancer Biology (Cancer Course 07226) |
EXTRAMURAL TEACHING: |
Graduate Student Education |
1998 - 2992 Faculty of Chemistry, Technical University of Lodz, Poland, Taught during Ph.D. studies |
2002 - 2004 Faculty of Chemistry, Technical University of Lodz, Poland, Lecturer |
11/2014 - 12/2014 Aix-Marseille Universite, Marseille, France, Visiting professor, Gave two lectures on the experimental methods in the research on reactive oxygen and nitrogen species in biological systems, consultations, development of collaborative projects |
MCW STUDENTS, FACULTY, RESIDENTS AND CLINICAL/RESEARCH FELLOWS MENTORED: |
High School Students |
Mackenzie Joranlien, Medical College of Wisconsin, 2019 Students Understanding Principles of Research Education through Medicine, Engineering, and Science (SUPREMES), Redox cycling activity of mito-nitropyridine in a cell-free system |
Katherine Schoenbeck, Medical College of Wisconsin, 2019 Students Understanding Principles of Research Education through Medicine, Engineering, and Science (SUPREMES), Kinetics and equilibrium of the formation of boronate-based prochelators – boronate-assisted click chemistry |
Ashley Tarcin, Medical College of Wisconsin, 2020 Students Understanding Principles of Research Education through Medicine, Engineering, and Science (SUPREMES), Phenazine methosulfate as a NAD(P)H oxidase mimetic and potential anticancer agent |
Undergraduate Students |
Asha Bailey, Medical College of Wisconsin, 2015 Diversity Summer Health-related Research Education Program (DSHREP) Students, Application of red-fluorescent boronates towards peroxynitrite and other oxidants *Helped mentor |
Marisol Madrigal, Medical College of Wisconsin, 2016 Diversity Summer Health-related Research Education Program (DSHREP) Students, Redox signaling induction in pancreatic cancer cells by mitochondria targeted metformin *Helped mentor |
Jayla Watkins, Medical College of Wisconsin, 2017 Diversity Summer Health-related Research Education Program (DSHREP) Students, Inhibitory effects of mitochondria-targeted agents on the production of peroxynitrite by activated macrophages *Helped mentor |
Esther Severe, Medical College of Wisconsin, 2019 Diversity Summer Health-related Research Education Program (DSHREP) Students, Redox cycling activity of mitochondria-targeted naphthoquinone |
Postdoctoral Students |
Adam Sikora, Medical College of Wisconsin, 2008 - 2009, Chemistry of the reaction of boronates towards peroxynitrite and other oxidants *Helped mentor |
Radoslaw Michalski, Medical College of Wisconsin, 2011 - 2012, (1) Development of a cell-impermeable probe for superoxide radical anion, (2) Development of a new assay for amino acid and protein hydroperoxides *Helped mentor |
Teruaki Koto, Medical College of Wisconsin, 2012 - 2013, Detection and identification of oxidants formed during •NO/O2•– reaction *Helped mentor |
Radoslaw Podsiadly, Medical college of Wisconsin, 2014 - 2015, Mechanistic aspects of the detection of biologically-relevant oxidants by peroxy-caged luciferin (PCL-1) probe *Helped mentor |
Aleksandra Grzelakowska, Medical College of Wisconsin, 2019, In vivo-compatible boronate-based probes for inflammatory oxidants |
TRAINING ACTIVITIES : |
2007 - 2018 Trained numerous internal and external researchers in the field of free radical research and in the services provided by the Free Radical Research Center |
BIBLIOGRAPHY |
Refereed Journal Publications/Original Papers |
1. Marcinek A, Zielonka J, Adamus J, Gȩbicki J, Platz MS. Direct characterization of radical species generated on one-electron oxidation of 3,6-diamino-10-methylacridan. J Phys Chemy A. 2001;105(5):875-9. |
2. Marcinek A, Zielonka J, Gȩbicki J, Gordon CM, Dunkin IR. Ionic liquids: Novel media for characterization of radical ions. Journal of Physical Chemistry A. 2001;105(40):9305-9. |
3. Gȩbicki J, Adamus J, Marcinek A, Zielonka J, Sysa-Jȩdrzejowska A, Woźniacka A, Denys A, Ciebiada I, Szmigiero L, Ciesielska E, Wieczorkowska M. In search for new drugs: From basic research to market product. Przemysl Chemiczny. 2003;82(8-9):507-10. |
4. Gebicki J, Sysa-Jedrzejowska A, Adamus J, Woźniacka A, Rybak M, Zielonka J. 1-Methylnicotinamide: a potent anti-inflammatory agent of vitamin origin. Pol J Pharmacol. 2003;55(1):109-12. |
5. Podsiady R, Sokoowska J, Marcinek A, Zielonka J, Chrześcijańska E, Socha A. Electrochemical and photochemical reduction of a series of azobenzene dyes in protic and aprotic solvents. Coloration Technology. 2003;119(5):269-74. |
6. Rogowski J, Zielonka J, Marcinek A, Gȩbicki J, Bednarek P. Benzopinacol radical cation. Journal of Physical Chemistry A. 2003;107(6):810-4. doi: 10.1021/jp021348e. |
7. Wieczorkowska M, Zielonka J, Celińska J, Adamus J, Ciesielska E, Marcinek A, Szmigiero L, Gebicki J. 1-Methyl-3-nitropyridine: an efficient oxidant of NADH in non-enzymatic and enzyme-mediated processes. Free Radic Res. 2003 Nov;37(11):1157-62. |
8. Zielonka J, Gebicki J, Grynkiewicz G. Radical scavenging properties of genistein. Free Radic Biol Med. 2003 Oct 15;35(8):958-65. |
9. Zielonka J, Marcinek A, Adamus J, Gȩbicki J. Direct observation of NADH radical cation generated in reactions with one-electron oxidants. Journal of Physical Chemistry A. 2003;107(46):9860-4. doi: 10.1021/jp035803y. |
10. Gebicki J, Marcinek A, Zielonka J. Transient species in the stepwise interconversion of NADH and NAD+. Acc Chem Res. 2004 Jun;37(6):379-86. |
11. Zielonka J, Podsiad R, Czerwińska M, Sikora A, Sokołowska J, Marcinek A. Color changes accompanying one-electron reduction and oxidation of the zero dyes. Journal of Photochemistry and Photobiology A: Chemistry. 2004;163(3):373-9. doi: 10.1016/j.jphotochem.2004.01.006. |
12. Zielonka J, Zhao H, Xu Y, Kalyanaraman B. Mechanistic similarities between oxidation of hydroethidine by Fremy's salt and superoxide: stopped-flow optical and EPR studies. Free Radic Biol Med. 2005 Oct 01;39(7):853-63. |
13. Podsiadły R, Sokołowska J, Marcinek A, Zielonka J, Socha A, Kaźmierska M. The relationship between the electrochemical and photochemical reduction of some azo dyes derived from 2-aminobenzothiazole. Journal of Photochemistry and Photobiology A: Chemistry. 2005;171(1):69-76. doi: 10.1016/j.jphotochem.2004.09.009. |
14. Czerwinska M, Sikora A, Szajerski P, Zielonka J, Adamus J, Marcinek A, Piech K, Bednarek P, Bally T. Anthralin: primary products of its redox reactions. J Org Chem. 2006 Jul 07;71(14):5312-9. |
15. Huh J, Liepins A, Zielonka J, Andrekopoulos C, Kalyanaraman B, Sorokin A. Cyclooxygenase 2 rescues LNCaP prostate cancer cells from sanguinarine-induced apoptosis by a mechanism involving inhibition of nitric oxide synthase activity. Cancer Res. 2006 Apr 01;66(7):3726-36. |
16. Szajerski P, Zielonka J, Sikora A, Adamus J, Marcinek A, Gebicki J, Kozlovski VI, Drelicharz L, Chłopicki S. Radical scavenging and NO-releasing properties of selected beta-adrenoreceptor antagonists. Free Radic Res. 2006 Jul;40(7):741-52. |
17. Zielonka J, Rybak M, Celińska J, Adamus J, Marcinek A, Gebicki J. Effect of heparin on viologen-stimulated enzymatic NADH depletion. Chem Res Toxicol. 2006 May;19(5):668-73. |
18. Zielonka J, Sarna T, Roberts JE, Wishart JF, Kalyanaraman B. Pulse radiolysis and steady-state analyses of the reaction between hydroethidine and superoxide and other oxidants. Arch Biochem Biophys. 2006 Dec 01;456(1):39-47. PMCID: PMC2270254 |
19. Zielonka J, Vasquez-Vivar J, Kalyanaraman B. The confounding effects of light, sonication, and Mn(III)TBAP on quantitation of superoxide using hydroethidine. Free Radic Biol Med. 2006 Oct 01;41(7):1050-7. |
20. Thomas S, Kotamraju S, Zielonka J, Harder DR, Kalyanaraman B. Hydrogen peroxide induces nitric oxide and proteosome activity in endothelial cells: a bell-shaped signaling response. Free Radic Biol Med. 2007 Apr 01;42(7):1049-61. PMCID: PMC2692187 |
21. Medhora M, Chen Y, Gruenloh S, Harland D, Bodiga S, Zielonka J, Gebremedhin D, Gao Y, Falck JR, Anjaiah S, Jacobs ER. 20-HETE increases superoxide production and activates NAPDH oxidase in pulmonary artery endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2008 May;294(5):L902-11. PMCID: PMC2586843 |
22. Sikora A, Szajerski P, Piotrowski L, Zielonka J, Adamus J, Marcinek A, Gebicki J. Radical scavenging properties of nicotinamide and its metabolites. Radiation Physics and Chemistry. 2008;77(3):259-66. doi: 10.1016/j.radphyschem.2007.05.005. |
23. Tang PC, Qin L, Zielonka J, Zhou J, Matte-Martone C, Bergaya S, van Rooijen N, Shlomchik WD, Min W, Sessa WC, Pober JS, Tellides G. MyD88-dependent, superoxide-initiated inflammation is necessary for flow-mediated inward remodeling of conduit arteries. J Exp Med. 2008 Dec 22;205(13):3159-71. PMCID: PMC2605224 |
24. Vásquez-Vivar J, Whitsett J, Ionova I, Konorev E, Zielonka J, Kalyanaraman B, Shi Y, Pieper GM. Cytokines and lipopolysaccharides induce inducible nitric oxide synthase but not enzyme activity in adult rat cardiomyocytes. Free Radic Biol Med. 2008 Oct 01;45(7):994-1001. PMCID: PMC2578873 |
25. Zielonka J, Kalyanaraman B. "ROS-generating mitochondrial DNA mutations can regulate tumor cell metastasis"--a critical commentary. Free Radic Biol Med. 2008 Nov 01;45(9):1217-9. PMCID: PMC3595710 |
26. Zielonka J, Srinivasan S, Hardy M, Ouari O, Lopez M, Vasquez-Vivar J, Avadhani NG, Kalyanaraman B. Cytochrome c-mediated oxidation of hydroethidine and mito-hydroethidine in mitochondria: identification of homo- and heterodimers. Free Radic Biol Med. 2008 Mar 01;44(5):835-46. PMCID: PMC2692199 |
27. Zielonka J, Vasquez-Vivar J, Kalyanaraman B. Detection of 2-hydroxyethidium in cellular systems: a unique marker product of superoxide and hydroethidine. Nat Protoc. 2008;3(1):8-21. |
28. Amour J, Brzezinska AK, Weihrauch D, Billstrom AR, Zielonka J, Krolikowski JG, Bienengraeber MW, Warltier DC, Pratt PF Jr, Kersten JR. Role of heat shock protein 90 and endothelial nitric oxide synthase during early anesthetic and ischemic preconditioning. Anesthesiology. 2009 Feb;110(2):317-25. PMCID: PMC2730207 |
29. Chandran K, Aggarwal D, Migrino RQ, Joseph J, McAllister D, Konorev EA, Antholine WE, Zielonka J, Srinivasan S, Avadhani NG, Kalyanaraman B. Doxorubicin inactivates myocardial cytochrome c oxidase in rats: cardioprotection by Mito-Q. Biophys J. 2009 Feb 18;96(4):1388-98. PMCID: PMC2717244 |
30. Sikora A, Zielonka J, Lopez M, Joseph J, Kalyanaraman B. Direct oxidation of boronates by peroxynitrite: mechanism and implications in fluorescence imaging of peroxynitrite. Free Radic Biol Med. 2009 Nov 15;47(10):1401-7. PMCID: PMC3375817 |
31. Zhang H, Zielonka J, Sikora A, Joseph J, Xu Y, Kalyanaraman B. The effect of neighboring methionine residue on tyrosine nitration and oxidation in peptides treated with MPO, H2O2, and NO2(-) or peroxynitrite and bicarbonate: role of intramolecular electron transfer mechanism? Arch Biochem Biophys. 2009 Apr 15;484(2):134-45. PMCID: PMC2874256 |
32. Zielonka J, Hardy M, Kalyanaraman B. HPLC study of oxidation products of hydroethidine in chemical and biological systems: ramifications in superoxide measurements. Free Radic Biol Med. 2009 Feb 01;46(3):329-38. PMCID: PMC3375818 |
33. Rao VA, Klein SR, Bonar SJ, Zielonka J, Mizuno N, Dickey JS, Keller PW, Joseph J, Kalyanaraman B, Shacter E. The antioxidant transcription factor Nrf2 negatively regulates autophagy and growth arrest induced by the anticancer redox agent mitoquinone. J Biol Chem. 2010 Nov 05;285(45):34447-59. PMCID: PMC2966059 |
34. Zielonka J, Kalyanaraman B. Hydroethidine- and MitoSOX-derived red fluorescence is not a reliable indicator of intracellular superoxide formation: another inconvenient truth. Free Radic Biol Med. 2010 Apr 15;48(8):983-1001. PMCID: PMC3587154 |
35. Zielonka J, Sikora A, Joseph J, Kalyanaraman B. Reply to Jourd'heuil: Peroxynitrite is the major species formed from different flux ratios of co-generated nitric oxide and superoxide. Journal of Biological Chemistry. 2010;285(35). doi: 10.1074/jbc.N110.110080. |
36. Zielonka J, Sikora A, Joseph J, Kalyanaraman B. Peroxynitrite is the major species formed from different flux ratios of co-generated nitric oxide and superoxide: direct reaction with boronate-based fluorescent probe. J Biol Chem. 2010 May 07;285(19):14210-6. PMCID: PMC2863194 |
37. Amour J, Brzezinska AK, Weihrauch D, Billstrom AR, Zielonka J, Krolikowski JG, Bienengraeber MW, Warltier DC, Pratt Jr PF, Kersten JR. Erratum: Role of heat shock protein 90 and endothelial nitric oxide synthase during early anesthetic and ischemic preconditioning (Anesthesiology (2009) 110 (317-325)). Anesthesiology. 2011;115(2):452. doi: 10.1097/ALN.0b013e31822b3e7b. |
38. Cheng G, Lopez M, Zielonka J, Hauser AD, Joseph J, McAllister D, Rowe JJ, Sugg SL, Williams CL, Kalyanaraman B. Mitochondria-targeted nitroxides exacerbate fluvastatin-mediated cytostatic and cytotoxic effects in breast cancer cells. Cancer Biol Ther. 2011 Oct 15;12(8):707-17. PMCID: PMC3218525 |
39. Myers JM, Antholine WE, Zielonka J, Myers CR. The iron-chelating drug triapine causes pronounced mitochondrial thiol redox stress. Toxicol Lett. 2011 Mar 05;201(2):130-6. |
40. Sikora A, Zielonka J, Lopez M, Dybala-Defratyka A, Joseph J, Marcinek A, Kalyanaraman B. Reaction between peroxynitrite and boronates: EPR spin-trapping, HPLC Analyses, and quantum mechanical study of the free radical pathway. Chem Res Toxicol. 2011 May 16;24(5):687-97. PMCID: PMC3743543 |
41. Cheng G, Zielonka J, Dranka BP, McAllister D, Mackinnon AC Jr, Joseph J, Kalyanaraman B. Mitochondria-targeted drugs synergize with 2-deoxyglucose to trigger breast cancer cell death. Cancer Res. 2012 May 15;72(10):2634-44. PMCID: PMC3700358 |
42. Dranka BP, Zielonka J, Kanthasamy AG, Kalyanaraman B. Alterations in bioenergetic function induced by Parkinson's disease mimetic compounds: lack of correlation with superoxide generation. J Neurochem. 2012 Sep;122(5):941-51. PMCID: PMC3423581 |
43. Mukhopadhyay P, Horváth B, Zsengellér Z, Zielonka J, Tanchian G, Holovac E, Kechrid M, Patel V, Stillman IE, Parikh SM, Joseph J, Kalyanaraman B, Pacher P. Mitochondrial-targeted antioxidants represent a promising approach for prevention of cisplatin-induced nephropathy. Free Radic Biol Med. 2012 Jan 15;52(2):497-506. PMCID: PMC3253235 |
44. Zielonka J, Sikora A, Hardy M, Joseph J, Dranka BP, Kalyanaraman B. Boronate probes as diagnostic tools for real time monitoring of peroxynitrite and hydroperoxides. Chem Res Toxicol. 2012 Sep 17;25(9):1793-9. PMCID: PMC3501381 |
45. Zielonka J, Zielonka M, Sikora A, Adamus J, Joseph J, Hardy M, Ouari O, Dranka BP, Kalyanaraman B. Global profiling of reactive oxygen and nitrogen species in biological systems: high-throughput real-time analyses. J Biol Chem. 2012 Jan 27;287(5):2984-95. PMCID: PMC3270955 |
46. Cheng G, Zielonka J, McAllister DM, Mackinnon AC Jr, Joseph J, Dwinell MB, Kalyanaraman B. Mitochondria-targeted vitamin E analogs inhibit breast cancer cell energy metabolism and promote cell death. BMC Cancer. 2013 Jun 13;13:285. PMCID: PMC3686663 |
47. Dickey JS, Gonzalez Y, Aryal B, Mog S, Nakamura AJ, Redon CE, Baxa U, Rosen E, Cheng G, Zielonka J, Parekh P, Mason KP, Joseph J, Kalyanaraman B, Bonner W, Herman E, Shacter E, Rao VA. Mito-tempol and dexrazoxane exhibit cardioprotective and chemotherapeutic effects through specific protein oxidation and autophagy in a syngeneic breast tumor preclinical model. PLoS One. 2013;8(8):e70575. PMCID: PMC3734284 |
48. Dranka BP, Gifford A, Ghosh A, Zielonka J, Joseph J, Kanthasamy AG, Kalyanaraman B. Diapocynin prevents early Parkinson's disease symptoms in the leucine-rich repeat kinase 2 (LRRK2R¹⁴⁴¹G) transgenic mouse. Neurosci Lett. 2013 Aug 09;549:57-62. PMCID: PMC3729885 |
49. Michalski R, Zielonka J, Hardy M, Joseph J, Kalyanaraman B. Hydropropidine: a novel, cell-impermeant fluorogenic probe for detecting extracellular superoxide. Free Radic Biol Med. 2013 Jan;54:135-47. PMCID: PMC3711142 |
50. Sikora A, Zielonka J, Adamus J, Debski D, Dybala-Defratyka A, Michalowski B, Joseph J, Hartley RC, Murphy MP, Kalyanaraman B. Reaction between peroxynitrite and triphenylphosphonium-substituted arylboronic acid isomers: identification of diagnostic marker products and biological implications. Chem Res Toxicol. 2013 Jun 17;26(6):856-67. PMCID: PMC3700382 |
51. Zielonka J, Joseph J, Sikora A, Kalyanaraman B. Real-time monitoring of reactive oxygen and nitrogen species in a multiwell plate using the diagnostic marker products of specific probes. Methods Enzymol. 2013;526:145-57. PMCID: PMC6941666 |
52. Zielonka J, Lambeth JD, Kalyanaraman B. On the use of L-012, a luminol-based chemiluminescent probe, for detecting superoxide and identifying inhibitors of NADPH oxidase: a reevaluation. Free Radic Biol Med. 2013 Dec;65:1310-1314. PMCID: PMC4274999 |
53. Cheng G, Zielonka J, McAllister D, Tsai S, Dwinell MB, Kalyanaraman B. Profiling and targeting of cellular bioenergetics: inhibition of pancreatic cancer cell proliferation. Br J Cancer. 2014 Jul 08;111(1):85-93. PMCID: PMC4090735 |
54. Dranka BP, Gifford A, McAllister D, Zielonka J, Joseph J, O'Hara CL, Stucky CL, Kanthasamy AG, Kalyanaraman B. A novel mitochondrially-targeted apocynin derivative prevents hyposmia and loss of motor function in the leucine-rich repeat kinase 2 (LRRK2(R1441G)) transgenic mouse model of Parkinson's disease. Neurosci Lett. 2014 Nov 07;583:159-64. PMCID: PMC4253647 |
55. Hardy M, Poulhés F, Rizzato E, Rockenbauer A, Banaszak K, Karoui H, Lopez M, Zielonka J, Vasquez-Vivar J, Sethumadhavan S, Kalyanaraman B, Tordo P, Ouari O. Mitochondria-targeted spin traps: synthesis, superoxide spin trapping, and mitochondrial uptake. Chem Res Toxicol. 2014 Jul 21;27(7):1155-65. PMCID: PMC5452977 |
56. Kalyanaraman B, Dranka BP, Hardy M, Michalski R, Zielonka J. HPLC-based monitoring of products formed from hydroethidine-based fluorogenic probes--the ultimate approach for intra- and extracellular superoxide detection. Biochim Biophys Acta. 2014 Feb;1840(2):739-44. PMCID: PMC3858408 |
57. Koto T, Michalski R, Zielonka J, Joseph J, Kalyanaraman B. Detection and identification of oxidants formed during •NO/O2•⁻ reaction: a multi-well plate CW-EPR spectroscopy combined with HPLC analyses. Free Radic Res. 2014 Apr;48(4):478-86. PMCID: PMC4780754 |
58. Kumar A, Chen SH, Kadiiska MB, Hong JS, Zielonka J, Kalyanaraman B, Mason RP. Inducible nitric oxide synthase is key to peroxynitrite-mediated, LPS-induced protein radical formation in murine microglial BV2 cells. Free Radic Biol Med. 2014 Aug;73:51-9. PMCID: PMC4111989 |
59. Michalski R, Michalowski B, Sikora A, Zielonka J, Kalyanaraman B. On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: a reassessment. Free Radic Biol Med. 2014 Feb;67:278-84. PMCID: PMC4275029 |
60. Michalski R, Zielonka J, Gapys E, Marcinek A, Joseph J, Kalyanaraman B. Real-time measurements of amino acid and protein hydroperoxides using coumarin boronic acid. J Biol Chem. 2014 Aug 08;289(32):22536-53. PMCID: PMC4139259 |
61. Shah G, Zielonka J, Chen F, Zhang G, Cao Y, Kalyanaraman B, See W. H2O2 generation by bacillus Calmette-Guérin induces the cellular oxidative stress response required for bacillus Calmette-Guérin direct effects on urothelial carcinoma biology. J Urol. 2014 Oct;192(4):1238-48. PMCID: PMC5798233 |
62. Smulik R, Dębski D, Zielonka J, Michałowski B, Adamus J, Marcinek A, Kalyanaraman B, Sikora A. Nitroxyl (HNO) reacts with molecular oxygen and forms peroxynitrite at physiological pH. Biological Implications. J Biol Chem. 2014 Dec 19;289(51):35570-81. PMCID: PMC4271240 |
63. Zielonka J, Cheng G, Zielonka M, Ganesh T, Sun A, Joseph J, Michalski R, O'Brien WJ, Lambeth JD, Kalyanaraman B. High-throughput assays for superoxide and hydrogen peroxide: design of a screening workflow to identify inhibitors of NADPH oxidases. J Biol Chem. 2014 Jun 06;289(23):16176-89. PMCID: PMC4047388 |
64. Cheng G, Zielonka J, McAllister D, Hardy M, Ouari O, Joseph J, Dwinell MB, Kalyanaraman B. Antiproliferative effects of mitochondria-targeted cationic antioxidants and analogs: Role of mitochondrial bioenergetics and energy-sensing mechanism. Cancer Lett. 2015 Aug 28;365(1):96-106. PMCID: PMC4476640 |
65. Debowska K, Debski D, Hardy M, Jakubowska M, Kalyanaraman B, Marcinek A, Michalski R, Michalowski B, Ouari O, Sikora A, Smulik R, Zielonka J. Toward selective detection of reactive oxygen and nitrogen species with the use of fluorogenic probes--Limitations, progress, and perspectives. Pharmacol Rep. 2015 Aug;67(4):756-64. PMCID: PMC5793900 |
66. Zielonka J, Sikora A, Adamus J, Kalyanaraman B. Detection and differentiation between peroxynitrite and hydroperoxides using mitochondria-targeted arylboronic acid. Methods Mol Biol. 2015;1264:171-81. PMCID: PMC4451192 |
67. Cheng G, Zielonka J, Ouari O, Lopez M, McAllister D, Boyle K, Barrios CS, Weber JJ, Johnson BD, Hardy M, Dwinell MB, Kalyanaraman B. Mitochondria-Targeted Analogues of Metformin Exhibit Enhanced Antiproliferative and Radiosensitizing Effects in Pancreatic Cancer Cells. Cancer Res. 2016 Jul 01;76(13):3904-15. PMCID: PMC4930686 |
68. Dębski D, Smulik R, Zielonka J, Michałowski B, Jakubowska M, Dębowska K, Adamus J, Marcinek A, Kalyanaraman B, Sikora A. Mechanism of oxidative conversion of Amplex® Red to resorufin: Pulse radiolysis and enzymatic studies. Free Radic Biol Med. 2016 Jun;95:323-32. PMCID: PMC5697983 |
69. Kalyanaraman B, Hardy M, Zielonka J. A Critical Review of Methodologies to Detect Reactive Oxygen and Nitrogen Species Stimulated by NADPH Oxidase Enzymes: Implications in Pesticide Toxicity. Curr Pharmacol Rep. 2016 Aug;2(4):193-201. PMCID: PMC5070669 |
70. Zielonka J, Podsiadły R, Zielonka M, Hardy M, Kalyanaraman B. On the use of peroxy-caged luciferin (PCL-1) probe for bioluminescent detection of inflammatory oxidants in vitro and in vivo - Identification of reaction intermediates and oxidant-specific minor products. Free Radic Biol Med. 2016 Oct;99:32-42. PMCID: PMC5107150 |
71. Zielonka J, Zielonka M, VerPlank L, Cheng G, Hardy M, Ouari O, Ayhan MM, Podsiadły R, Sikora A, Lambeth JD, Kalyanaraman B. Mitigation of NADPH Oxidase 2 Activity as a Strategy to Inhibit Peroxynitrite Formation. J Biol Chem. 2016 Mar 25;291(13):7029-44. PMCID: PMC4807286 |
72. Egea J, Fabregat I, Frapart YM, Ghezzi P, Görlach A, Kietzmann T, Kubaichuk K, Knaus UG, Lopez MG, Olaso-Gonzalez G, Petry A, Schulz R, Vina J, Winyard P, Abbas K, Ademowo OS, Afonso CB, Andreadou I, Antelmann H, Antunes F, Aslan M, Bachschmid MM, Barbosa RM, Belousov V, Berndt C, Bernlohr D, Bertrán E, Bindoli A, Bottari SP, Brito PM, Carrara G, Casas AI, Chatzi A, Chondrogianni N, Conrad M, Cooke MS, Costa JG, Cuadrado A, My-Chan Dang P, De Smet B, Debelec-Butuner B, Dias IHK, Dunn JD, Edson AJ, El Assar M, El-Benna J, Ferdinandy P, Fernandes AS, Fladmark KE, Förstermann U, Giniatullin R, Giricz Z, Görbe A, Griffiths H, Hampl V, Hanf A, Herget J, Hernansanz-Agustín P, Hillion M, Huang J, Ilikay S, Jansen-Dürr P, Jaquet V, Joles JA, Kalyanaraman B, Kaminskyy D, Karbaschi M, Kleanthous M, Klotz LO, Korac B, Korkmaz KS, Koziel R, Kračun D, Krause KH, Křen V, Krieg T, Laranjinha J, Lazou A, Li H, Martínez-Ruiz A, Matsui R, McBean GJ, Meredith SP, Messens J, Miguel V, Mikhed Y, Milisav I, Milković L, Miranda-Vizuete A, Mojović M, Monsalve M, Mouthuy PA, Mulvey J, Münzel T, Muzykantov V, Nguyen ITN, Oelze M, Oliveira NG, Palmeira CM, Papaevgeniou N, Pavićević A, Pedre B, Peyrot F, Phylactides M, Pircalabioru GG, Pitt AR, Poulsen HE, Prieto I, Rigobello MP, Robledinos-Antón N, Rodríguez-Mañas L, Rolo AP, Rousset F, Ruskovska T, Saraiva N, Sasson S, Schröder K, Semen K, Seredenina T, Shakirzyanova A, Smith GL, Soldati T, Sousa BC, Spickett CM, Stancic A, Stasia MJ, Steinbrenner H, Stepanić V, Steven S, Tokatlidis K, Tuncay E, Turan B, Ursini F, Vacek J, Vajnerova O, Valentová K, Van Breusegem F, Varisli L, Veal EA, Yalçın AS, Yelisyeyeva O, Žarković N, Zatloukalová M, Zielonka J, Touyz RM, Papapetropoulos A, Grune T, Lamas S, Schmidt HHHW, Di Lisa F, Daiber A. European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS). Redox Biol. 2017 Oct;13:94-162. PMCID: PMC5458069 |
73. Egea J, Fabregat I, Frapart YM, Ghezzi P, Görlach A, Kietzmann T, Kubaichuk K, Knaus UG, Lopez MG, Olaso-Gonzalez G, Petry A, Schulz R, Vina J, Winyard P, Abbas K, Ademowo OS, Afonso CB, Andreadou I, Antelmann H, Antunes F, Aslan M, Bachschmid MM, Barbosa RM, Belousov V, Berndt C, Bernlohr D, Bertrán E, Bindoli A, Bottari SP, Brito PM, Carrara G, Casas AI, Chatzi A, Chondrogianni N, Conrad M, Cooke MS, Costa JG, Cuadrado A, My-Chan Dang P, De Smet B, Debelec-Butuner B, Dias IHK, Dunn JD, Edson AJ, El Assar M, El-Benna J, Ferdinandy P, Fernandes AS, Fladmark KE, Förstermann U, Giniatullin R, Giricz Z, Görbe A, Griffiths H, Hampl V, Hanf A, Herget J, Hernansanz-Agustín P, Hillion M, Huang J, Ilikay S, Jansen-Dürr P, Jaquet V, Joles JA, Kalyanaraman B, Kaminskyy D, Karbaschi M, Kleanthous M, Klotz LO, Korac B, Korkmaz KS, Koziel R, Kračun D, Krause KH, Křen V, Krieg T, Laranjinha J, Lazou A, Li H, Martínez-Ruiz A, Matsui R, McBean GJ, Meredith SP, Messens J, Miguel V, Mikhed Y, Milisav I, Milković L, Miranda-Vizuete A, Mojović M, Monsalve M, Mouthuy PA, Mulvey J, Münzel T, Muzykantov V, Nguyen ITN, Oelze M, Oliveira NG, Palmeira CM, Papaevgeniou N, Pavićević A, Pedre B, Peyrot F, Phylactides M, Pircalabioru GG, Pitt AR, Poulsen HE, Prieto I, Rigobello MP, Robledinos-Antón N, Rodríguez-Mañas L, Rolo AP, Rousset F, Ruskovska T, Saraiva N, Sasson S, Schröder K, Semen K, Seredenina T, Shakirzyanova A, Smith GL, Soldati T, Sousa BC, Spickett CM, Stancic A, Stasia MJ, Steinbrenner H, Stepanić V, Steven S, Tokatlidis K, Tuncay E, Turan B, Ursini F, Vacek J, Vajnerova O, Valentová K, Van Breusegem F, Varisli L, Veal EA, Yalçın AS, Yelisyeyeva O, Žarković N, Zatloukalová M, Zielonka J, Touyz RM, Papapetropoulos A, Grune T, Lamas S, Schmidt HHHW, Di Lisa F, Daiber A. Corrigendum to "European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS)" [Redox Biol. 13 (2017) 94-162]. Redox Biol. 2018 Apr;14:694-696. PMCID: PMC5975209 |
74. Hardy M, Zielonka J, Karoui H, Sikora A, Michalski R, Podsiadły R, Lopez M, Vasquez-Vivar J, Kalyanaraman B, Ouari O. Detection and Characterization of Reactive Oxygen and Nitrogen Species in Biological Systems by Monitoring Species-Specific Products. Antioxid Redox Signal. 2018 May 20;28(15):1416-1432. PMCID: PMC5910052 |
75. Kalyanaraman B, Cheng G, Hardy M, Ouari O, Lopez M, Joseph J, Zielonka J, Dwinell MB. A review of the basics of mitochondrial bioenergetics, metabolism, and related signaling pathways in cancer cells: Therapeutic targeting of tumor mitochondria with lipophilic cationic compounds. Redox Biol. 2018 Apr;14:316-327. PMCID: PMC5633086 |
76. Kalyanaraman B, Cheng G, Hardy M, Ouari O, Sikora A, Zielonka J, Dwinell M. Mitochondria-targeted metformins: anti-tumour and redox signalling mechanisms. Interface Focus. 2017 Apr 06;7(2):20160109. PMCID: PMC5311906 |
77. Kalyanaraman B, Cheng G, Hardy M, Ouari O, Lopez M, Joseph J, Zielonka J, Dwinell MB. Corrigendum to 'A review of the basics of mitochondrial bioenergetics, metabolism, and related signaling pathways in cancer cells: Therapeutic targeting of tumor mitochondria with lipophilic cationic compounds' [REDOX 14C (2017) 316-327]. Redox Biol. 2018 Jun;16:426-427. PMCID: PMC5952812 |
78. Kalyanaraman B, Cheng G, Hardy M, Ouari O, Sikora A, Zielonka J, Dwinell MB. Modified Metformin as a More Potent Anticancer Drug: Mitochondrial Inhibition, Redox Signaling, Antiproliferative Effects and Future EPR Studies. Cell Biochem Biophys. 2017 Dec;75(3-4):311-317. PMCID: PMC5680142 |
79. Zielonka J, Hardy M, Michalski R, Sikora A, Zielonka M, Cheng G, Ouari O, Podsiadły R, Kalyanaraman B. Recent Developments in the Probes and Assays for Measurement of the Activity of NADPH Oxidases. Cell Biochem Biophys. 2017 Dec;75(3-4):335-349. PMCID: PMC5693611 |
80. Kalyanaraman B, Zielonka J. Green fluorescent proteins induce oxidative stress in cells: A worrisome new wrinkle in the application of the GFP reporter system to biological systems? Redox Biol. 2017 Aug;12:755-757. PMCID: PMC5397015 |
81. Langley M, Ghosh A, Charli A, Sarkar S, Ay M, Luo J, Zielonka J, Brenza T, Bennett B, Jin H, Ghaisas S, Schlichtmann B, Kim D, Anantharam V, Kanthasamy A, Narasimhan B, Kalyanaraman B, Kanthasamy AG. Mito-Apocynin Prevents Mitochondrial Dysfunction, Microglial Activation, Oxidative Damage, and Progressive Neurodegeneration in MitoPark Transgenic Mice. Antioxid Redox Signal. 2017 Nov 10;27(14):1048-1066. PMCID: PMC5651937 |
82. Kalyanaraman B, Hardy M, Podsiadly R, Cheng G, Zielonka J. Recent developments in detection of superoxide radical anion and hydrogen peroxide: Opportunities, challenges, and implications in redox signaling. Arch Biochem Biophys. 2017 Mar 01;617:38-47. PMCID: PMC5318280 |
83. Zielonka J, Joseph J, Sikora A, Hardy M, Ouari O, Vasquez-Vivar J, Cheng G, Lopez M, Kalyanaraman B. Mitochondria-Targeted Triphenylphosphonium-Based Compounds: Syntheses, Mechanisms of Action, and Therapeutic and Diagnostic Applications. Chem Rev. 2017 Aug 09;117(15):10043-10120. PMCID: PMC5611849 |
84. Kalyanaraman B, Cheng G, Hardy M, Ouari O, Bennett B, Zielonka J. Teaching the basics of reactive oxygen species and their relevance to cancer biology: Mitochondrial reactive oxygen species detection, redox signaling, and targeted therapies. Redox Biol. 2018 May;15:347-362. PMCID: PMC5756055 |
85. Xu Z, Ni B, Cao Z, Zielonka J, Gao J, Chen F, Kalyanaraman B, White GC, Ma YQ. Kindlin-3 negatively regulates the release of neutrophil extracellular traps. J Leukoc Biol. 2018 Sep;104(3):597-602. PMCID: PMC6113072 |
86. Zielonka J, Kalyanaraman B. Small-molecule luminescent probes for the detection of cellular oxidizing and nitrating species. Free Radic Biol Med. 2018 Nov 20;128:3-22. PMCID: PMC6146080 |
87. Pan J, Lee Y, Cheng G, Zielonka J, Zhang Q, Bajzikova M, Xiong D, Tsaih S-W, Hardy M, Flister M, Olsen CM, Wang Y, Vang O, Neuzil J, Myers CR, Kalyanaraman B, You M. Mitochondria-Targeted Honokiol Confers a Striking Inhibitory Effect on Lung Cancer via Inhibiting Complex I Activity. iScience. 2018;3:192-207. doi: 10.1016/j.isci.2018.04.013. |
88. Cheng G, Zielonka M, Dranka B, Kumar SN, Myers CR, Bennett B, Garces AM, Dias Duarte Machado LG, Thiebaut D, Ouari O, Hardy M, Zielonka J, Kalyanaraman B. Detection of mitochondria-generated reactive oxygen species in cells using multiple probes and methods: Potentials, pitfalls, and the future. J Biol Chem. 2018 Jun 29;293(26):10363-10380. PMCID: PMC6028982 |
89. Kalyanaraman B, Cheng G, Zielonka J, Bennett B. Low-Temperature EPR Spectroscopy as a Probe-Free Technique for Monitoring Oxidants Formed in Tumor Cells and Tissues: Implications in Drug Resistance and OXPHOS-Targeted Therapies. Cell Biochem Biophys. 2019 Mar;77(1):89-98. PMCID: PMC6430651 |
90. Radi R, Denicola A, Morgan B, Zielonka J. Foreword to the Free Radical Biology and Medicine Special Issue on ¨Current fluorescence and chemiluminescence approaches in free radical and redox biology¨. Free Radic Biol Med. 2018 Nov 20;128:1-2. |
91. Pan J, Lee Y, Cheng G, Zielonka J, Zhang Q, Bajzikova M, Xiong D, Tsaih SW, Hardy M, Flister M, Olsen CM, Wang Y, Vang O, Neuzil J, Myers CR, Kalyanaraman B, You M. Mitochondria-Targeted Honokiol Confers a Striking Inhibitory Effect on Lung Cancer via Inhibiting Complex I Activity. iScience. 2018 May 25;3:192-207. PMCID: PMC6137433 |
92. Cheng G, Zielonka J, Hardy M, Ouari O, Chitambar CR, Dwinell MB, Kalyanaraman B. Synergistic inhibition of tumor cell proliferation by metformin and mito-metformin in the presence of iron chelators. Oncotarget. 2019 May 28;10(37):3518-3532. PMCID: PMC6544408 |
93. Besson E, Gastaldi S, Bloch E, Zielonka J, Zielonka M, Kalyanaraman B, Aslan S, Karoui H, Rockenbauer A, Ouari O, Hardy M. Embedding cyclic nitrone in mesoporous silica particles for EPR spin trapping of superoxide and other radicals. Analyst. 2019 Jul 21;144(14):4194-4203. |
94. Zielonka J, Zielonka M, Cheng G, Hardy M, Kalyanaraman B. High-Throughput Screening of NOX Inhibitors. Methods Mol Biol. 2019;1982:429-446. PMCID: PMC6953630 |
95. Zielonka J, Zielonka M, Kalyanaraman B. HPLC-Based Monitoring of Oxidation of Hydroethidine for the Detection of NADPH Oxidase-Derived Superoxide Radical Anion. Methods Mol Biol. 2019;1982:243-258. PMCID: PMC6953631 |
96. Cheng G, Zhang Q, Pan J, Lee Y, Ouari O, Hardy M, Zielonka M, Myers CR, Zielonka J, Weh K, Chang AC, Chen G, Kresty L, Kalyanaraman B, You M. Targeting lonidamine to mitochondria mitigates lung tumorigenesis and brain metastasis. Nat Commun. 2019 May 17;10(1):2205. PMCID: PMC6525201 |
97. Hogg N, Zielonka J, Kalyanaraman B. Detection of Nitric Oxide and Peroxynitrite in Biological Systems: A State-of-the-Art Review Nitric Oxide: Biology and Pathobiology: Third Edition. 1 January 2017:23-44. |
98. Zielonka J, Hardy M, Kalyanaraman B. Corrigendum to "HPLC study of oxidation products of hydroethidine in chemical and biological systems: Ramifications in superoxide measurements" [Free Radic. Biol. Med. 46 (2009) 329-338] (DOI:10.1016/j.freeradbiomed.2008.10.031) Free Radical Biology and Medicine. 15 January 2010;48(2):373. |
99. He C, Danes JM, Hart PC, Zhu Y, Huang Y, de Abreu AL, O'Brien J, Mathison AJ, Tang B, Frasor JM, Wakefield LM, Ganini D, Stauder E, Zielonka J, Gantner BN, Urrutia RA, Gius D, Bonini MG. SOD2 acetylation on lysine 68 promotes stem cell reprogramming in breast cancer. Proc Natl Acad Sci U S A. 2019 Nov 19;116(47):23534-23541. PMCID: PMC6876149 |
100. Cheng G, Pan J, Podsiadly R, Zielonka J, Garces AM, Dias Duarte Machado LG, Bennett B, McAllister D, Dwinell MB, You M, Kalyanaraman B. Increased formation of reactive oxygen species during tumor growth: Ex vivo low-temperature EPR and in vivo bioluminescence analyses. Free Radic Biol Med. 2020 Feb 01;147:167-174. PMCID: PMC6948008 |
101. Rios N, Radi R, Kalyanaraman B, Zielonka J. Tracking isotopically labeled oxidants using boronate-based redox probes. J Biol Chem. 2020 May 08;295(19):6665-6676. PMCID: PMC7212653 |
102. Zhang Q, Cheng G, Pan J, Zielonka J, Xiong D, Myers CR, Feng L, Shin SS, Kim YH, Bui D, Hu M, Bennett B, Schmainda K, Wang Y, Kalyanaraman B, You M. Magnolia extract is effective for the chemoprevention of oral cancer through its ability to inhibit mitochondrial respiration at complex I. Cell Commun Signal. 2020 Apr 07;18(1):58. PMCID: PMC7140380 |
103. Chowdhury AR, Zielonka J, Kalyanaraman B, Hartley RC, Murphy MP, Avadhani NG. Mitochondria-targeted paraquat and metformin mediate ROS production to induce multiple pathways of retrograde signaling: A dose-dependent phenomenon. Redox Biol. 2020 Sep;36:101606. PMCID: PMC7327929 |
104. Szala M, Grzelakowska A, Modrzejewska J, Siarkiewicz P, Słowiński D, Świerczyńska M, Zielonka J, Podsiadły R. Characterization of the reactivity of luciferin boronate - A probe for inflammatory oxidants with improved stability Dyes and Pigments. December 2020;183. |
105. Angireddy R, Chowdhury AR, Zielonka J, Ruthel G, Kalyanaraman B, Avadhani NG. Alcohol-induced CYP2E1, mitochondrial dynamics and retrograde signaling in human hepatic 3D organoids. Free Radic Biol Med. 2020 Nov 01;159:1-14. |
106. Yang M, Li W, Harberg C, Chen W, Yue H, Ferreira RB, Wynia-Smith SL, Carroll KS, Zielonka J, Flaumenhaft R, Silverstein RL, Smith BC. Cysteine sulfenylation by CD36 signaling promotes arterial thrombosis in dyslipidemia. Blood Adv. 2020 Sep 22;4(18):4494-4507. PMCID: PMC7509873 |
107. Cheng G, Hardy M, Zielonka J, Weh K, Zielonka M, Boyle KA, Abu Eid M, McAllister D, Bennett B, Kresty LA, Dwinell MB, Kalyanaraman B. Mitochondria-targeted magnolol inhibits OXPHOS, proliferation, and tumor growth via modulation of energetics and autophagy in melanoma cells. Cancer Treat Res Commun. 2020;25:100210. PMCID: PMC7883397 |
108. Sikora A, Zielonka J, Dębowska K, Michalski R, Smulik-Izydorczyk R, Pięta J, Podsiadły R, Artelska A, Pierzchała K, Kalyanaraman B. Boronate-Based Probes for Biological Oxidants: A Novel Class of Molecular Tools for Redox Biology. Front Chem. 2020;8:580899. PMCID: PMC7545953 |
109. Michalski R, Thiebaut D, Michałowski B, Ayhan MM, Hardy M, Ouari O, Rostkowski M, Smulik-Izydorczyk R, Artelska A, Marcinek A, Zielonka J, Kalyanaraman B, Sikora A. Oxidation of ethidium-based probes by biological radicals: mechanism, kinetics and implications for the detection of superoxide. Sci Rep. 2020 Oct 29;10(1):18626. PMCID: PMC7596101 |
110. Kalinovic S, Stamm P, Oelze M, Steven S, Kröller-Schön S, Kvandova M, Zielonka J, Münzel T, Daiber A. Detection of extracellular superoxide in isolated human immune cells and in an animal model of arterial hypertension using hydropropidine probe and HPLC analysis. Free Radic Biol Med. 2021 May 20;168:214-225. |
111. Siarkiewicz P, Michalski R, Sikora A, Smulik-Izydorczyk R, Szala M, Grzelakowska A, Modrzejewska J, Bailey A, Nycz JE, Kalyanaraman B, Malecki JG, Zielonka J, Podsiadły R. On the chemical reactivity of tricyanofuran(TCF)-based near-infrared fluorescent redox probes – Effects of glutathione on the probe response and product fluorescence Dyes and Pigments. August 2021;192. |
112. Medhora M, Phadnis P, Narayanan J, Gasperetti T, Zielonka J, Moulder JE, Fish BL, Szabo A. Radiation Increases Bioavailability of Lisinopril, a Mitigator of Radiation-Induced Toxicities Frontiers in Pharmacology. 27 April 2021;12. |
113. Grzelakowska A, Zielonka M, Dębowska K, Modrzejewska J, Szala M, Sikora A, Zielonka J, Podsiadły R. Two-photon fluorescent probe for cellular peroxynitrite: Fluorescence detection, imaging, and identification of peroxynitrite-specific products. Free Radic Biol Med. 2021 Jun;169:24-35. |
114. Medhora M, Phadnis P, Narayanan J, Gasperetti T, Zielonka J, Moulder JE, Fish BL, Szabo A. Radiation Increases Bioavailability of Lisinopril, a Mitigator of Radiation-Induced Toxicities. Front Pharmacol. 2021;12:646076. PMCID: PMC8111401 |
115. AbuEid M, McAllister DM, McOlash L, Harwig MC, Cheng G, Drouillard D, Boyle KA, Hardy M, Zielonka J, Johnson BD, Hill RB, Kalyanaraman B, Dwinell MB. Synchronous effects of targeted mitochondrial complex I inhibitors on tumor and immune cells abrogate melanoma progression iScience. 25 June 2021;24(6). |
116. Zielonka J, Sikora A, Podsiadly R, Hardy M, Kalyanaraman B. Identification of Peroxynitrite by Profiling Oxidation and Nitration Products from Mitochondria-Targeted Arylboronic Acid. Methods Mol Biol. 2021;2275:315-327. PMCID: PMC9245525 |
117. AbuEid M, McAllister DM, McOlash L, Harwig MC, Cheng G, Drouillard D, Boyle KA, Hardy M, Zielonka J, Johnson BD, Hill RB, Kalyanaraman B, Dwinell MB. Synchronous effects of targeted mitochondrial complex I inhibitors on tumor and immune cells abrogate melanoma progression. iScience. 2021 Jun 25;24(6):102653. PMCID: PMC8220235 |
118. Modrzejewska J, Szala M, Grzelakowska A, Zakłos-Szyda M, Zielonka J, Podsiadły R. Novel Boronate Probe Based on 3-Benzothiazol-2-yl-7-hydroxy-chromen-2-one for the Detection of Peroxynitrite and Hypochlorite. Molecules. 2021 Sep 30;26(19). PMCID: PMC8512868 |
119. Kalinovic S, Stamm P, Oelze M, Steven S, Kröller-Schön S, Kvandova M, Zielonka J, Münzel T, Daiber A. Detection of extracellular superoxide in isolated human immune cells and in an animal model of arterial hypertension using hydropropidine probe and HPLC analysis. Free Radic Biol Med. 2021 May 20;168:214-225. |
120. Grzelakowska A, Modrzejewska J, Kolińska J, Szala M, Zielonka M, Dębowska K, Zakłos-Szyda M, Sikora A, Zielonka J, Podsiadły R. Water-soluble cationic boronate probe based on coumarin imidazolium scaffold: Synthesis, characterization, and application to cellular peroxynitrite detection. Free Radic Biol Med. 2022 Feb 01;179:34-46. |
121. Pierzchała K, Pięta M, Rola M, Świerczyńska M, Artelska A, Dębowska K, Podsiadły R, Pięta J, Zielonka J, Sikora A, Marcinek A, Michalski R. Fluorescent probes for monitoring myeloperoxidase-derived hypochlorous acid: a comparative study. Sci Rep. 2022 Jun 03;12(1):9314. PMCID: PMC9166712 |
122. Kim J, Yang Y, Hong SK, Zielonka J, Dash RK, Audi SH, Kumar SN, Joshi A, Zimmerman MA, Hong JC. Fluorescein clearance kinetics in blood and bile indicates hepatic ischemia-reperfusion injury in rats. Am J Physiol Gastrointest Liver Physiol. 2022 Aug 01;323(2):G126-G133. |
123. Vujacic-Mirski K, Oelze M, Kuntic I, Kuntic M, Kalinovic S, Li H, Zielonka J, M??nzel T, Daiber A. Measurement of Tetrahydrobiopterin in Animal Tissue Samples by HPLC with Electrochemical Detectiona??Protocol Optimization and Pitfalls Antioxidants. June 2022;11(6). |
124. Michalski R, Smulik-Izydorczyk R, Pięta J, Rola M, Artelska A, Pierzchała K, Zielonka J, Kalyanaraman B, Sikora AB. The Chemistry of HNO: Mechanisms and Reaction Kinetics. Front Chem. 2022;10:930657. PMCID: PMC9294461 |
125. Vujacic-Mirski K, Oelze M, Kuntic I, Kuntic M, Kalinovic S, Li H, Zielonka J, Münzel T, Daiber A. Measurement of Tetrahydrobiopterin in Animal Tissue Samples by HPLC with Electrochemical Detection-Protocol Optimization and Pitfalls. Antioxidants (Basel). 2022 Jun 16;11(6). PMCID: PMC9228106 |
126. Patel JJ, Willoughby R, Peterson J, Carver T, Zelten J, Markiewicz A, Spiegelhoff K, Hipp LA, Canales B, Szabo A, Heyland DK, Stoppe C, Zielonka J, Freed JK. High-Dose IV Hydroxocobalamin (Vitamin B12) in Septic Shock: A Double-Blind, Allocation-Concealed, Placebo-Controlled Single-Center Pilot Randomized Controlled Trial (The Intravenous Hydroxocobalamin in Septic Shock Trial). Chest. 2023 Feb;163(2):303-312. |
127. Huang L, Cheng F, Zhang X, Zielonka J, Nystoriak MA, Xiang W, Raygor K, Wang S, Lakshmanan A, Jiang W, Yuan S, Hou KS, Zhang J, Wang X, Syed AU, Juric M, Takahashi T, Navedo MF, Wang RA. Nitric oxide synthase and reduced arterial tone contribute to arteriovenous malformation. Sci Adv. 2023 May 26;9(21):eade7280. PMCID: PMC10219588 |
128. Pierzchała K, Pięta J, Pięta M, Rola M, Zielonka J, Sikora A, Marcinek A, Michalski R. Boronate-Based Oxidant-Responsive Derivatives of Acetaminophen as Proinhibitors of Myeloperoxidase. Chem Res Toxicol. 2023 Aug 21;36(8):1398-1408. PMCID: PMC10445283 |
129. Juric M, Rawat V, Amaradhi R, Zielonka J, Ganesh T. Novel NADPH Oxidase-2 Inhibitors as Potential Anti-Inflammatory and Neuroprotective Agents Antioxidants. September 2023;12(9). |
130. Uche N, Dai Q, Lai S, Kolander K, Thao M, Schibly E, Sendaydiego X, Zielonka J, Benjamin IJ. Carvedilol Phenocopies PGC-1α Overexpression to Alleviate Oxidative Stress, Mitochondrial Dysfunction and Prevent Doxorubicin-Induced Toxicity in Human iPSC-Derived Cardiomyocytes. Antioxidants (Basel). 2023 Aug 09;12(8). PMCID: PMC10451268 |
131. Zielonka J, Juric M. Methods to Measure Reactive Oxygen Species Production by NADPH Oxidases NADPH Oxidases Revisited: From Function to Structure. 1 January 2023:323-341. |
132. Juric M, Rawat V, Amaradhi R, Zielonka J, Ganesh T. Novel NADPH Oxidase-2 Inhibitors as Potential Anti-Inflammatory and Neuroprotective Agents. Antioxidants (Basel). 2023 Aug 23;12(9). PMCID: PMC10525516 |
133. Szala M, Grzelakowska A, Michalski R, Dębowska K, Modrzejewska J, Adamczyk M, Siarkiewicz P, Sikora A, Zielonka J, Podsiadły R. Synthesis and characterization of the reactivity of new far-red fluorescent dyes and a boronate probe containing a tricyanopyrroline skeleton Dyes and Pigments. March 2024;222. |
134. Sørensen M, Pershagen G, Thacher JD, Lanki T, Wicki B, Röösli M, Vienneau D, Cantuaria ML, Schmidt JH, Aasvang GM, Al-Kindi S, Osborne MT, Wenzel P, Sastre J, Fleming I, Schulz R, Hahad O, Kuntic M, Zielonka J, Sies H, Grune T, Frenis K, Münzel T, Daiber A. Health position paper and redox perspectives - Disease burden by transportation noise. Redox Biol. 2024 Feb;69:102995. PMCID: PMC10788624 |
135. Siarkiewicz P, Luzak B, Michalski R, Artelska A, Szala M, Przygodzki T, Sikora A, Zielonka J, Grzelakowska A, Podsiadły R. Evaluation of a novel pyridinium cation-linked styryl-based boronate probe for the detection of selected inflammation-related oxidants. Free Radic Biol Med. 2024 Feb 20;212:255-270. |
136. Pant T, Uche N, Juric M, Zielonka J, Bai X. Regulation of immunomodulatory networks by Nrf2-activation in immune cells: Redox control and therapeutic potential in inflammatory diseases. Redox Biol. 2024 Apr;70:103077. PMCID: PMC10877431 |
Books, Chapters, and Reviews |
1. Zielonka J, Kalyanaraman B. “Methods of Investigation of Selected Radical Oxygen/Nitrogen Species in Cell-free and Cellular Systems” in: Principles of Free Radical Biomedicine, Volume 1, K. Pantopoulos and H.M. Schipper (eds.), Nova Science Publishers, 2011. |
2. Zielonka J, Sikora A, Adamus A, Kalyanaraman B, “Detection and differentiation between peroxynitrite and hydroperoxides using mitochondria-targeted arylboronic acid” in: Mitochondrial Medicine, V. Weissig and M. Edeas (eds.), Springer, 2015. |
3. Hogg N, Zielonka J, Kalyanaraman B. “Detection of Nitric Oxide and Peroxynitrite in Biological Systems: A State-of-the-Art Review” in: Nitric Oxide, 3rd Edition. Biology and Pathobiology, L. Ignarro and B. Freeman (eds.), Elsevier, 2017. |
4. Zielonka J, Zielonka M, Cheng G, Hardy M, Kalyanaraman B. High-Throughput Screening of NOX Inhibitors. In: Knaus UG, Leto TL, editors. NADPH Oxidases: Methods and Protocols. New York, NY: Springer New York; 2019. |
5. Zielonka J, Zielonka M, Kalyanaraman B. HPLC-Based Monitoring of Oxidation of Hydroethidine for the Detection of NADPH Oxidase-Derived Superoxide Radical Anion. In: Knaus UG, Leto TL, editors. NADPH Oxidases: Methods and Protocols. New York, NY: Springer New York; 2019. |
Abstracts |
1. Kalyanaraman B, Zielonka J, Vasquez-Vivar J. THE CONFOUNDING EFFECTS OF LIGHT, SONICATION, AND MnTBAP ON FORMATION OF 2-HYDROXYETHIDIUM, THE PRODUCT OF HYDROETHIDINE AND SUPEROXIDE REACTION, AS DETECTED BY HPLC-EC: 7. Free Radical Biology and Medicine. 2005;39. |
2. Sarna T, Zielonka J, Roberts J, Wishart J, Kalyanaraman B. PULSE RADIOLYSIS MEASUREMENTS OF THE RATE CONSTANTS BETWEEN HYDROETHIDINE AND SUPEROXIDE: 313. Free Radical Biology and Medicine. 2005;39. |
3. Zielonka J, Vasquez-Vivar J, Kalyanaraman B. DETECTION OF INTRACELLULAR SUPEROXIDE WITH HYDROETHIDINE AND MITOSOX™: 317. Free Radical Biology and Medicine. 2005;39. |
4. Hardy M, Vasquez-Vivar J, Zielonka J, Kalyanaraman B. The Use of Mito-DEPMPO and Mito-SOX in Superoxide Detection from Uncoupled Nitric Oxide Synthase: 263. Free Radical Biology and Medicine. 2006;41. |
5. Kalyanaraman B, Hardy M, Lopez M, Vazquez-Vivar J, Rockenbauer A, Zielonka J, Srinivasan S, Avadhani N, Felix C, Tordo P. Detection, Characterization and Decay Kinetics of ROS and Thiyl Adducts of Mito-DEPMPO, a Novel Mitochondria-Targeted Spin Trap: 159. Free Radical Biology and Medicine. 2006;41. |
6. Kotamraju S, Zielonka J, Bright M, Williams C, Kalyanaraman B. Role of Arginine Metabolizing Enzymes and NADPH Oxidase in Statin-Induced Tumor Cell Cytotoxicity: 285. Free Radical Biology and Medicine. 2006;41. |
7. Zhang H, Xu Y, Zielonka J, Wishart J, Sarna T, Kalyanaraman B. The Constrasting Influence of Cysteine and Methionine on Nitration of Neighboring Tyrosine in Model Peptides: 167. Free Radical Biology and Medicine. 2006;41. |
8. Zielonka J, Sarna T, Roberts J, Wishart J, Kalyanaraman B. The Reaction of Superoxide with Hydroethidine and Its Mitochondria-Targeted Analog: Pulse Radiolysis and Steady-State Analyses: 265. Free Radical Biology and Medicine. 2006;41. |
9. Flewelen T, Leigh N, Zielonka J, Hillery C, Kalyanaraman B, Hogg N. The Oxidative Potential of Cell-Free Hemoglobin in Sickle Cell Disease: 94. Free Radical Biology and Medicine. 2007;43. |
10. Larsen BT, Phillips SA, Bubolz AH, Zielonka J, Kalyanaraman B, Gutterman DD. HPLC separation of hydroethidine products: a specific and sensitive method for quantifying microvascular superoxide. The FASEB Journal. 2007;21(5):A447-A. |
11. Prah D, Paulson E, Zielonka J, Hardy M, Joseph J, Kalyanaraman B, Schmainda K, editors. In Vivo Mitochondrial Labeling Using Mito-Carboxy Proxyl (Mito-CP) Enhanced Magnetic Resonance Imaging. Proc Intl Soc Mag Reson Med; 2007. |
12. Rao A, Klein S, Zielonka J, Kalyanaraman B, Shacter E. The Mitochondrially-Targeted Redox Agent Mitoquinone Enhances Doxorubicin-Induced Toxicity to Breast Cancer Cells While Protecting Cardiac Myocytes: 133. Free Radical Biology and Medicine. 2007;43. |
13. Zielonka J, Srinivasan S, Hardy M, Ouari O, Lopez M, Vasquez-Vivar J, Avadhani N, Kalyanaraman B. Detection of Dimeric Products from HE and its Mitochondria-Targeted Analog Induced by Ferricytcochrome C and Ferricyanide: 476. Free Radical Biology and Medicine. 2007;43. |
14. Lopez M, Prah D, Hardy M, Zielonka J, Paulson E, Schmainda K, Joseph J, Kalyanaraman B. Mitochondria-Targeted Nitroxides As MRI Contrast Agents and Chemotherapeutics: 131. Free Radical Biology and Medicine. 2008;45. |
15. Sikora A, Zielonka J, Piotrowski L, Andrekopoulos C, Joseph J, Adamus J, Marcinek A, Li A, Kalyanaraman B. Apocynin Inhibits Carbonate Radical-Induced Aggregation of Human Cu, Zn-SOD: 239. Free Radical Biology and Medicine. 2008;45. |
16. Joseph J, Lopez M, Cheng G, Zielonka J, Ramadoss J, McAllister D, Chandel N, Kalyanaraman B. Mitochondria-targeted Nitroxide Mitigates Doxorubicin Toxicity in Cardiomyocites but Exacerbates Breast Tumor Cell Cytotoxicity: 470. Free Radical Biology and Medicine. 2009;47. |
17. Sikora A, Zielonka J, Joseph J, Kalyanaraman B. Oxidation of Coumarin Boronate to Hydroxycoumarin by Different Fluxes of Nitric Oxide and Superoxide: Quantitative Measurements of Peroxynitrite under Various Nitric Oxide/Superoxide Fluxes: 72. Free Radical Biology and Medicine. 2009;47. |
18. Zielonka J, Sikora A, Zielonka M, Joseph J, Hardy M, Kalyanaraman B. Monitoring the Peroxynitrite Generation by Activated Macrophages: The Use of Novel Boronate-Based Fluorogenic Probes: 82. Free Radical Biology and Medicine. 2009;47. |
19. Cheng G, Zielonka J, Dranka BP, Joseph J, Kalyanaraman B. Uptake, Subcellular Localization, Bioenergetic Effects and Antiproliferative Properties of Triphenylphosphoniumlinked Nitroxides in Breast Cancer Cells. Free Radical Biology and Medicine. 2010(49):S57. |
20. Dranka BP, Zielonka J, Kanthasamy AG, Kalyanaraman B. Bioenergetic Remodeling Induced by 1-methyl-4-phenylpyridinium (MPP+) in Dopaminergic N27 cells: Implications for Parkinson's Disease. Free Radical Biology and Medicine. 2010(49):S154-S5. |
21. Joseph J, Kim J-H, Cheng G, Zielonka J, Kalyanaraman B. Enhanced Antiproliferative and Cytotoxic Effects of Mitochondria-targeted Tocopherol in Breast Cancer Cells. Free Radical Biology and Medicine. 2010(49):S63. |
22. Sikora A, Dybala-Defratyka A, Zielonka J, Marcinek A, Kalyanaraman B. The Mechanism of the Oxidative Transformation of Boronate Compounds-A Quantum Mechanical Study. Free Radical Biology and Medicine. 2010(49):S216. |
23. Zhao B, Ranguelova K, Jiang J, Zielonka J, Kalyanaraman B, Ronald MP. Studies on the photosensitized reduction of resorufin and implications for the detection of oxidative stress with Amplex red. Free Radical Biology and Medicine. 2010(49):S105-S6. |
24. Zielonka J, Dranka BP, Zielonka M, Joseph J, Hardy M, Sikora A, Kalyanaraman B. Reduction of Mitochondrial Reserve Capacity in Endothelial Cells by Nitric Oxide and Superoxide-Detection and Quantitation of Peroxynitrite Formed from Cogenerated Nitric Oxide and Superoxide. Free Radical Biology and Medicine. 2010(49):S123-S4. |
25. Zielonka J, Zielonka M, Sikora A, Adamus J, Joseph J, Hardy M, Ouari O, Kalyanaraman B. Global Profiling of Reactive Oxygen and Nitrogen Species in Cell-free and Model Cellular Systems-Real Time Fluorescence Monitoring and HPLC Measurements. Free Radical Biology and Medicine. 2010(49):S106. |
26. Cheng G, McAllister D, Zielonka J, Dranka BP, Joseph J, Kalyanaraman B. Synergistic Enhancement of 2-Deoxy-D-Glucose-Mediated Breast Cancer Cell Death by Mitochondria-Targeted Drugs. Free Radical Biology and Medicine. 2011(51):S120. |
27. Cheng G, Zielonka J, Joseph J, Kalyanaraman B. Synergistic Lethality of Inhibitors of Bioenergetic Pathways in Pancreatic Cells. Free Radical Biology and Medicine. 2011(51):S120. |
28. Cheng G, Zielonka J, Kalyanaraman B. Modulatory effects of MitoSOX on cellular bioenergetics: a cautionary note. Free Radical Biology and Medicine. 2011(51):S37-S8. |
29. Dranka BP, Zielonka J, Hardy M, Michalski R, Kalyanaraman B. A Novel Approach for Detection of Superoxide-Dependent 2-Hydroxyethidium Fluorescence Using Lambda Mode Confocal Microscopy. Free Radical Biology and Medicine. 2011(51):S133. |
30. Dranka BP, Zielonka J, Kalyanaraman B. Mitochondrial Inhibition and Oxidant Production Differs Between Pharmacologic Models of Parkinson's Disease. Free Radical Biology and Medicine. 2011(51):S69. |
31. Joseph J, Zielonka J, Pacher P, Kalyanaraman B. Syntheses and Applications of Mitochondria-Targeted Antioxidants. Free Radical Biology and Medicine. 2011(51):S88. |
32. Michalski R, Zielonka J, Hardy M, Kalyanaraman B. Development of a Novel Cell-Impermeable Fluorogenic Probe for Superoxide Radical Anion. Free Radical Biology and Medicine. 2011(51):S147-S8. |
33. Mukhopadhyay P, Horvath B, Zsengellér Z, Zielonka J, Tanchian G, Kechrid M, Patel V, Stillman IE, Parikh SM, Joseph J. Mitochondrial-Targeted Antioxidants Represent a Promising Approach for Prevention Of. Free Radical Biology and Medicine. 2011(51):S93. |
34. Peluffo G, Calcerrada P, Piacenza L, Zielonka J, Kalyanaraman B, Radi R. Use of Boron-Based Compounds for the Detection of Peroxynitrite in Vascular Endothelial Cells. Free Radical Biology and Medicine. 2011(51):S48. |
35. Sikora AB, Zielonka J, Adamus J, Debski D, Joseph J, Murphy MP, Kalyanaraman B. Different Reaction Profiles of Mitochondria-Targeted Arylboronic Acid Isomers With Peroxynitrite. Free Radical Biology and Medicine. 2011(51):S149-S50. |
36. Zielonka J, Cheng G, Kim J-H, Joseph J, Kalyanaraman B. Selective killing of breast cancer cells by mitochondria-targeted α-tocopherol. Free Radical Biology and Medicine. 2011(51):S130. |
37. Zielonka J, Kielar F, Sikora A, Franz KJ, Kalyanaraman B. Dual Antioxidant Activity of Boronate-Based Prochelators: Scavenging Peroxynitrite and Metal Ion Chelation. Free Radical Biology and Medicine. 2011(51):S100-S1. |
38. Zielonka J, Zielonka M, Ganesh T, Sun A, Lambeth D, Kalyanaraman B. Inhibitors of NADPH Oxidase Decrease Peroxynitrite Formation in Activated Macrophages. Free Radical Biology and Medicine. 2011(51):S53. |
39. Cheng G, Zielonka J, Dayton AR, Joseph J, Kalyanaraman B. Alterations in Kreb's cycle carbon pool and bioenergetics metabolites in pancreatic cancer cells treated with mitochondria-targeted drug. Free Radical Biology and Medicine. 2012(53):S40. |
40. Drechsel DA, Zielonka J, Kalyanaraman B, Beckman J. Peroxynitrite Formation from Re-Oxidation of Zinc-Deficient Superoxide Dismutase: Implications in Amyotrophic Lateral Sclerosis. Free Radical Biology and Medicine. 2012(53):S20. |
41. Michalski R, Zielonka J, Joseph J, Kalyanaraman B. A Novel Assay for Real-Time Monitoring of Tyrosine Hydroperoxide Using Coumarin Boronic Acid (CBA). Free Radical Biology and Medicine. 2012(53):S26. |
42. Sikora A, Piotrowski L, Debski D, Guminska K, Adamus J, Marcinek A, Zielonka J, Kalyanaraman B. Reactivity of Resorufin-Based Fluorogenic Probe PC1 toward ROS/RNS and its use in the Studies of Peroxynitrite Reactivity. Free Radical Biology and Medicine. 2012(53):S152. |
43. Debski D, Michalowski B, Adamus J, Wojciechowski J, Zielonka J, Kalyanaraman B, Sikora A. Direct characterization of transient species formed upon one-electron oxidation of Amplex Red. Free Radical Biology and Medicine. 2013(65):S88-S9. |
44. Michalski R, Gapys E, Sikora A, Kwiatkowski P, Adamus J, Zielonka J, Marcinek A, Kalyanaraman B. Reactivity of Salen-Manganese Complexes toward Amino Acid, Peptide and Protein Hydroperoxides. Free Radical Biology and Medicine. 2013(65):S145-S6. |
45. Pacher P, Mukhopadhyay P, Horvath B, Zsengeller Z, Zielonka J, Kechrid M, Stillman IE, Parikh SM, Joseph J, Kalyanaraman B. Mitochondrial‐targeted antioxidants represent a promising approach for prevention of cisplatin‐induced nephropathy. The FASEB Journal. 2013;27:889.3-.3. |
46. Shah G, Sack B, Cheng G, Zielonka J, Kalyanaraman B, See W. 589 BCG-INDUCED DEPLETION OF INTRACELLULAR ATP IN HUMAN UROTHELIAL CARCINOMA CELL LINES CORRELATES WITH CELLULAR INJURY AND CYTOTOXICITY. The Journal of Urology. 2013;189(4S):e241-e. |
47. Shah G, Zhang G, Chen F, Zielonka J, Kalyanaraman B, See W. 582 H2O2 GENERATION BY BCG CORRELATES WITH IN VITRO ANTITUMOR ACTIVITY. The Journal of Urology. 2013;189(4S):e238-e. |
48. Zhang G, Chen F, Cao Y, Zielonka J, Cheng G, Shah G, Kalyanaraman B, See W. 587 LOSS OF BCG VIABILITY ADVERSELY AFFECTS THE DIRECT RESPONSE OF UROTHELIAL CARCINOMA CELLS TO BCG EXPOSURE. The Journal of Urology. 2013;189(4S):e240-e. |
49. Zielonka J, Lambeth D, Kalyanaraman B. On the Chemiluminescent Probes for Superoxide: Is L-012 Probe a Reliable Tool for Detection and Quantification of O2•-? Free Radical Biology and Medicine. 2013(65):S94. |
50. Shah G, Chen F, Lockwood G, Johnson S, Zielonka J, Kalyanaraman B, See W. MP34-05 THE OXIDATIVE STRESS RESPONSE OF UROTHELIAL CARCINOMA CELLS TO BCG EXPOSURE. The Journal of Urology. 2014;191(4S):e363-e. |
51. Thao MT, Riedel M, Zielonka J, Vasquez-Vivar J, Hogg N, Kalyanaraman B, Benjamin I. Imaging the Dynamic Redox Changes of Endoplasmic Reticulum with Green Fluorescent Protein. Free Radical Biology and Medicine. 2014(76):S93. |
52. Zielonka J, Zielonka M, Hardy M, Ouari O, Kalyanaraman B. Spin Trapping with Fluorescence Detection: Novel Method for Specific and Sensitive Fluorimetric Detection of Superoxide Radical Anion. Free Radical Biology and Medicine. 2015;1(87):S19-S20. |
53. Harberg C, Yang M, Zielonka J, Silverstein RL. CD36 Activation Enhances Hydrogen Peroxide Generation in Human Platelets. Blood. 2017;130(Supplement 1):3605-. |
54. Yang M, Harberg C, Wynia-Smith S, Ferreira R, Zielonka J, Carroll K, Silverstein R, Smith B. Protein sulfenylation by oxidized lipid signaling promotes activation of platelets. Free Radical Biology and Medicine. 2018;128:S44-S5. |
55. Zielonka J, Sikora A, Pięta J, Zielonka M, Cheng G, Kalyanaraman B. Targeting redox-active pyridinium cations to mitochondria to inhibit proliferation of colon cancer cells. Free Radical Biology and Medicine. 2018;128:S78. |
56. Oehm S, Zielonka J, Kielar F, Sikora A, Franz K, Kalyanaraman B, editors. Dual anti-nitration and antioxidant activity of boronate-based prochelators: Kinetic and mechanistic aspects. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY; 2019: AMER CHEMICAL SOC 1155 16TH ST, NW, WASHINGTON, DC 20036 USA. |
57. Stauder E, Sikora A, Hardy M, Zielonka M, Kalyanaraman B, Zielonka J. Simultaneous Induction of Bioenergetic and Redox Stress as a Strategy to Inhibit Proliferation and Induce Death of Pancreatic Cancer Cells: 171. Free Radical Biology and Medicine. 2019;145. |
Patents |
1. Pending / MCW Tech ID: C1788
“Modified mito-metformin compounds and methods of synthesis and use thereof”
Inventors: Micael Hardy, Balaraman Kalyanaraman, Marcos Lopez, Olivier Ouari, Jacek Zielonka
|
2. Pending (filed June 10, 2016) / MCW Tech ID: C1820
“Mito-honokiol compounds and methods of synthesis and use thereof”
Inventors: Balaraman Kalyanaraman, Jacek M. Zielonka, Micael J. Hardy, Olivier Ouari, Ming You
|
3. Pending / MCW Tech ID: C1999
“Mito-magnolol compounds and methods of synthesis and use thereof”
Inventors: Balaraman Kalyanaraman, Jacek M. Zielonka, Gang Cheng, Micael J. Hardy, Olivier Ouari
|
4. Pending / MCW Tech ID: C2049
“Targeting redox-active pyridinium cations to mitochondria to inhibit proliferation of drug-resistant cancer cells”
Inventors: Jacek M. Zielonka, Adam B. Sikora, Balaraman Kalyanaraman |
