Distinguishing characteristics of a new neuroblastoma cell line. Cancer Genet Cytogenet 1983 Oct;10(2):177-86
Date
10/01/1983Pubmed ID
6616438DOI
10.1016/0165-4608(83)90122-xScopus ID
2-s2.0-0020603530 (requires institutional sign-in at Scopus site) 17 CitationsAbstract
The characteristics of a new neuroblastoma cell line (MC-NB-1) established from the bone marrow of a 2-year-old male are described. Morphologically, the cells appear as flattened and epithelial-like or as small and spherical. Electron microscopy demonstrated microtubules and dense core secretory granules. The doubling time was approximately 35 hr. Isoenzyme patterns and catecholamine secretion indicated a human line of neuronal origin. The soft agar tumor colony forming system demonstrated drug resistance in vitro comparable to in vivo nonresponsiveness. The stemline karyotype of MC-NB-1 is 44,Y,del(1) (p22:), -4, -7, +del(7)(q22:), -16, +t(7;16)(16pter leads to 16q24::7q22 leads to 7q32), -17. Additionally, double-minute bodies were observed. However, no evidence of homogeneous staining regions (HSRs) were detected.
Author List
Casper JT, Trent JM, Harb J, Piaskowski V, Helmsworth M, Finlan J, Von Hoff DDAuthor
James Casper MD Emeritus Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
CarboxylesteraseCarboxylic Ester Hydrolases
Catecholamines
Cell Line
Child, Preschool
Chromosome Banding
Humans
Isoenzymes
Karyotyping
Male
Neuroblastoma
