Differential sensitivity of mouse hematopoietic stem cells to merocyanine 540. Differentiation 1981;19(1):65-7
Date
01/01/1981Pubmed ID
7327314DOI
10.1111/j.1432-0436.1981.tb01129.xScopus ID
2-s2.0-0019508321 (requires institutional sign-in at Scopus site) 34 CitationsAbstract
In vivo and in vitro clonal assays of immature mouse blood cells showed that different populations of hematopoietic progenitor cells differ considerably with respect to their sensitivity to photodynamic damages caused by the fluorescent dye Merocyanine 540. Late erythroid progenitors were the most sensitive cells followed in order of decreasing sensitivity by pluripotent stem cells, early erythroid progenitors, and granulocyte/macrophage progenitors. Only about 2%-4% of all nucleated marrow cells were stained with Merocyanine 540 which correlated well with current frequency estimates of progenitor cells in mouse bone marrow. Our findings indicate that the expression of Merocyanine binding sites is developmentally regulated and might, therefore, provide a useful molecular marker for blood cell differentiation and a basis for an effective purification of hematopoietic progenitor cells.
Author List
Sieber F, Meagher RC, Spivak JLAuthor
Fritz Sieber PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBenzoxazoles
Cell Differentiation
Colony-Forming Units Assay
Dose-Response Relationship, Drug
Female
Fluorescent Dyes
Hematopoietic Stem Cells
Mice
Pyrimidinones
