Faculty Collaboration Database

Use of simple sequence length polymorphisms for genetic characterization of rat inbred strains. Mamm Genome 1995 Sep;6(9):595-601

Date

09/01/1995

Pubmed ID

8535065

DOI

10.1007/BF00352364

Scopus ID

2-s2.0-0029360661 (requires institutional sign-in at Scopus site)   15 Citations

Abstract

Genetic monitoring is an essential component of colony management and for the rat has been accomplished primarily by using immunological and biochemical markers. Here, we report that simple sequence length polymorphisms (SSLPs) are a faster and more economical way of monitoring inbred strains of rats. We characterized 61 inbred strains of rats, using primer pairs for 37 SSLPs. Each of these loci appeared to be highly polymorphic, with the number of alleles per locus ranging between 3 and 14 and, as a result, all the 61 inbred strains tested in this study could be provided with a unique strain profile. These strain profiles are also used for estimating the degree of similarity between strains. This information may provide the rationale in selecting strains for genetic crosses or for other specific purposes.

Author List

Otsen M, Den Bieman M, Winer ES, Jacob HJ, Szpirer J, Szpirer C, Bender K, Van Zutphen LF

MESH terms used to index this publication - Major topics in bold

Animals
Base Sequence
DNA Primers
Female
Hybrid Cells
Male
Microsatellite Repeats
Molecular Sequence Data
Polymorphism, Genetic
Rats
Rats, Inbred Strains
Reproducibility of Results