Pre-emptive placement of a presealant for amniotic access. Am J Obstet Gynecol 2005 Sep;193(3 Pt 2):1197-203
Date
09/15/2005Pubmed ID
16157137DOI
10.1016/j.ajog.2005.05.062Scopus ID
2-s2.0-24644486014 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
OBJECTIVE: Amniotic access for fetal diagnosis and therapy can lead to membrane leaks, separation, and preterm premature rupture of membranes. Morbidity limits the beneficial effects of fetal intervention. We propose to examine a novel preventive "presealant" membrane puncture technique and evaluate it in vitro.
STUDY DESIGN: Fetal membranes from normal term deliveries were fastened to a pressure controlled pump and punctured after presealant placement. Distinct bioadhesives were then compared for sealing efficacy by the measurement of leak pressures. Membranes were also evaluated for changes in tensile rupture strength after treatment.
RESULTS: Preemptive sealing by 2 of the presealants achieved significantly higher leak pressures compared with control membranes (85 and 78, respectively, vs 27 cm of water; P < .05). One of the presealants worked effectively in a membrane-sealant-membrane interface (62 vs 30 cm of water; P < .05). All treated membranes, however, demonstrated diminished tensile rupture forces compared with control membranes (124 vs 170 g/cm2; P > .05).
CONCLUSION: The application of a presealant is effective for the prevention of iatrogenic rupture in an in vitro model and may be beneficial for clinical application. Further studies with different bioadhesives are needed, given the limitations of the glues that are described in this study.
Author List
Cortes RA, Wagner AJ, Lee H, Clifton MS, Grethel E, Yang SH, Ball R, Harrison MRAuthor
Amy Wagner MD Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
CyanoacrylatesExtraembryonic Membranes
Female
Fetus
Humans
Pregnancy
Punctures
Tensile Strength
