Evaluation of merocyanine 540-sensitized photoirradiation as a method for purging malarially infected red cells from blood. J Infect Dis 1991 Jun;163(6):1312-7
Date
06/01/1991Pubmed ID
2037797DOI
10.1093/infdis/163.6.1312Scopus ID
2-s2.0-0025942788 (requires institutional sign-in at Scopus site) 16 CitationsAbstract
The photosensitizing dye merocyanine 540 (MC 540) was evaluated as a means for purging malarially infected red cells from murine blood using the rodent malarial pathogens, Plasmodium yoelii and Plasmodium berghei, as models of human malaria. Malarially infected red cells bound more MC 540 and were more sensitive to MC 540-sensitized photoirradiation than were noninfected erythroid cells. Extracorporeal exposure of infected red cells to the dye and white light prevented the transmission of the disease in a transfusion model. P. berghei-infected red cells were more resistant to the antimalarial activity of MC 540 than were P. yoelii-infected cells, presumably because P. berghei preferentially infects reticulocytes whereas P. yoelii infects mature red cells. The possibility of using photoirradiation sensitized by MC 540 or related dyes to purge malarially infected donor blood is discussed.
Author List
Smith OM, Traul DL, McOlash L, Sieber FAuthor
Fritz Sieber PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBlood Transfusion
Disease Models, Animal
Erythrocytes
Female
Flow Cytometry
Malaria
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Microscopy, Fluorescence
Photochemotherapy
Plasmodium
Plasmodium berghei
Plasmodium yoelii
Pyrimidinones
Radiation-Sensitizing Agents
