Medical College of Wisconsin
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Dye-mediated photolysis of normal and neoplastic hematopoietic cells. Leuk Res 1987;11(1):43-9

Date

01/01/1987

Pubmed ID

3807420

DOI

10.1016/0145-2126(87)90104-4

Scopus ID

2-s2.0-0023080569 (requires institutional sign-in at Scopus site)   65 Citations

Abstract

The purpose of this study was to determine the sensitivity to merocyanine 540 (MC 540)-mediated photolysis of normal human hematopoietic progenitor cells and four leukemia cell lines (Daudi, Raji, K562 and HL-60). Late erythroid progenitors were the most sensitive normal cells. Early erythroid progenitors were of intermediate sensitivity. Granulocyte/macrophage progenitors and multipotent progenitors were the least sensitive normal marrow cells. A combination of dye concentration, serum concentration, and illumination that eliminated 50% of multipotent progenitor cells reduced the concentration of leukemic cells by greater than or equal to 4.5 log. It is conceivable that this difference in photosensitivity can be exploited for the extracorporeal purging of autologous remission marrow grafts.

Author List

Sieber F, Stuart RK, Rowley SD, Sharkis SJ, Sensenbrenner LL

Author

Fritz Sieber PhD Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Bone Marrow Cells
Cells, Cultured
Hematopoietic Stem Cells
Humans
Kinetics
Leukemia
Photolysis
Pyrimidinones
Time Factors