Faculty Collaboration Database

Primary structure of the human platelet serotonin 5-HT2A receptor: identify with frontal cortex serotonin 5-HT2A receptor. J Neurochem 1994 Aug;63(2):465-9

Date

08/01/1994

Pubmed ID

8035173

DOI

10.1046/j.1471-4159.1994.63020465.x

Scopus ID

2-s2.0-0028016331 (requires institutional sign-in at Scopus site)   152 Citations

Abstract

Previous radioligand binding studies have demonstrated human platelet serotonin2A (5-HT2A) receptor binding sites. Pharmacological similarities between platelet and frontal cortex 5-HT2A receptor binding parameters have been demonstrated. However, it is not clear whether the platelet 5-HT2A receptor primary structure is identical to that of the brain receptor. Three overlapping cDNAs were obtained to span completely the coding region of the 5-HT2A receptor. These clones were sequenced with external and internal primers. The nucleotide sequence of human platelet 5-HT2A cDNA was identical to that reported for the human frontal cortex 5-HT2A receptor, except for nucleotide 102 (T-->C), which has been reported to represent a normal DNA polymorphism that does not alter the amino acid sequence. This finding may have implications in the study of neuropsychiatric disorders for which altered platelet 5-HT2A receptor binding has been demonstrated.

Author List

Cook EH Jr, Fletcher KE, Wainwright M, Marks N, Yan SY, Leventhal BL

Author

Kathlyn E. Fletcher MD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Amino Acid Sequence
Base Sequence
Blood Platelets
Frontal Lobe
Gene Expression
Humans
Molecular Sequence Data
Polymerase Chain Reaction
RNA Splicing
RNA, Messenger
Receptors, Serotonin