Antigenic variability of Aspergillus fumigatus strains. Microbios 1977;19(77-78):191-204
Date
01/01/1977Pubmed ID
82195Scopus ID
2-s2.0-0017564787 (requires institutional sign-in at Scopus site) 86 CitationsAbstract
The effect of culture media, temperature of incubation, and continuous shaking of cultures, on the reactivity and yield of antigens of Aspergillus fumigatus were evaluated. It was found that AOAC medium was superior to Czapek medium and shake cultures yielded better results than stationary cultures. However, antigens from stationary cultures in AOAC medium incubated at 30 degrees C for 3 weeks were equally as good as antigens obtained from 2-week-old shake cultures. Antigens from 11 selected strains of Aspergillus fumigatus were used to test antibody activity in 33 sera from patients with various forms of aspergillosis and 35 normal controls by the agar gel double-diffusion method. The results showed that the reactivity of individual antigens varied from 42 to 87%, indicating that antigens from more than one strain of Aspergillus fumigatus may be used. The cross-reactivity between strains were studied by two-dimensional immunoelectrophoresis. The use of polyacrylamide gel electrophoresis and crossed immunoelectrophoresis in the quality assurance of Aspergillus antigens is discussed.
Author List
Kurup VP, Fink JN, Scribner GH, Falk MJMESH terms used to index this publication - Major topics in bold
Antigens, FungalAspergillosis
Aspergillus fumigatus
Cross Reactions
Culture Media
Epitopes
Humans
Immunoelectrophoresis, Two-Dimensional
Temperature
