Ischemic preconditioning: triggering role of nitric oxide-derived oxidants in isolated hearts. J Cardiovasc Pharmacol 2003 Nov;42(5):593-600
Date
10/25/2003Pubmed ID
14576506DOI
10.1097/00005344-200311000-00003Scopus ID
2-s2.0-0142169946 (requires institutional sign-in at Scopus site) 19 CitationsAbstract
There is evidence that oxidants generated during ischemic preconditioning (IPC) trigger or mediate cardioprotection. We examined whether a causal relationship exists between oxidant formation during ischemic preconditioning and cardioprotection. We monitored formation of dityrosine in crystalloid-perfused guinea pig isolated hearts after a preconditioning protocol and after prolonged ischemia. Superoxide dismutase, catalase, and glutathione (SCG), or the L-arginine analogue NGnitro L-arginine methyl ester (L-NAME) were given during preconditioning. Dityrosine was observed in the coronary effluent immediately after both stimuli, but not after bracketing with SCG or L-NAME. After prolonged ischemia, dityrosine was significantly lower in the IPC group than in other groups. IPC was evidenced by improved mechanical and metabolic function on reperfusion, and by reduced infarction. These effects were abrogated by either SCG or L-NAME. These data support the hypothesis that the formation of nitric oxide-derived oxidants during ischemic preconditioning is causally related to myocardial adaptation to reperfusion injury.
Author List
Novalija E, Hogg N, Kevin LG, Camara AK, Stowe DFAuthors
Amadou K. Camara PhD Professor in the Anesthesiology department at Medical College of WisconsinNeil Hogg PhD Associate Dean, Professor in the Biophysics department at Medical College of Wisconsin
David F. Stowe MD, PhD Professor in the Anesthesiology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsGuinea Pigs
Heart
In Vitro Techniques
Ischemic Preconditioning, Myocardial
Myocardial Ischemia
NG-Nitroarginine Methyl Ester
Nitric Oxide
Oxidants
Perfusion
