Chromatography of human urinary erythropoietin and granulocyte colony-stimulating factor on insolubilized phytohaemagglutinin. Biochim Biophys Acta 1977 Jan 24;496(1):146-54
Date
01/24/1977Pubmed ID
300031DOI
10.1016/0304-4165(77)90122-2Scopus ID
2-s2.0-0017601431 (requires institutional sign-in at Scopus site) 3 CitationsAbstract
Human urinary erythropoetin was absorbed to phytohaemagglutinin coupled to agarose or porous glass and quatitatively eluted by a saturated solution of MgCl2. This method provides a means of separating erythropoietin from several of its contaminants, presumably on the basis of its carbohydrate side chains. Erythropoietin which had been purified by chromatography on insolubilized phytohaemagglutinin was sufficiently free of toxicity to be assayable in tissue culture even when crude urine was used as a starting material.
Author List
Sieber FAuthor
Fritz Sieber PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Chromatography, AffinityColony-Stimulating Factors
Erythropoietin
Humans
Lectins