Faculty Collaboration Database

ATP-induced chondrocalcinosis. Arthritis Rheum 1992 Dec;35(12):1520-5

Date

12/01/1992

Pubmed ID

1472129

DOI

10.1002/art.1780351216

Scopus ID

2-s2.0-0027074833 (requires institutional sign-in at Scopus site)   50 Citations

Abstract

OBJECTIVE: To determine whether adult articular cartilage mineralizes in the presence of ATP.

METHODS: Intact adult porcine articular cartilage and monolayers of chondrocytes were cultured in physiologic media containing ATP, and mineralization was measured as retention of 45Ca. Cartilage was analyzed by electron microscopy.

RESULTS: Articular cartilage sequestered 45Ca when incubated with 100 microM ATP: Use of the ATP analog alpha, beta-methylene ATP did not promote mineralization and addition of pyrophosphatase inhibited mineralization, indicating that hydrolysis of ATP to AMP and inorganic pyrophosphate is necessary for the process to occur. Mineral was concentrated in articular cartilage vesicles in the perichondral area.

CONCLUSION: Adult articular cartilage mineralizes in the presence of ATP, in a manner similar to that found with isolated matrix or articular cartilage vesicles. This supports the notion that these structures have a role in chondrocalcinosis.

Author List

Ryan LM, Kurup IV, Derfus BA, Kushnaryov VM

MESH terms used to index this publication - Major topics in bold

Adenosine Triphosphate
Animals
Calcification, Physiologic
Calcium
Calcium Phosphates
Calcium Pyrophosphate
Calcium Radioisotopes
Cartilage, Articular
Cells, Cultured
Chondrocalcinosis
Hydrolysis
Microscopy, Electron