Faculty Collaboration Database

Glucocorticoids modulate renal glucocorticoid receptors and Na-K ATPase activity. Kidney Int 1987 Oct;32(4):464-71

Date

10/01/1987

Pubmed ID

2828745

DOI

10.1038/ki.1987.233

Scopus ID

2-s2.0-0023203146 (requires institutional sign-in at Scopus site)   16 Citations

Abstract

Primary cultures of mouse renal tubular epithelial cells were used to study the effect of hydrocortisone on the regulation of glucocorticoid receptors (GR) and on sodium-potassium adenosine triphosphatase (Na-K ATPase) activity. A GR assay was developed and performed directly on cell monolayers maintained in serum-free medium to which hydrocortisone at 5 nM, 50 nM, and 5 X 10(-4) M was added. Compared with control cells grown in medium without hydrocortisone, GR levels per cell decreased by 50% after 48 hours of growth in medium containing 5 nM hydrocortisone concentrations (50 nM or 5 X 10(-4) M), GR levels decreased to less than or equal to 28% of control values. In all hydrocortisone treatment groups there was an inverse relation between GR concentrations and Na-K ATPase activity. Binding of cell GR by the addition of the antiglucocorticoid RU 38486 in hydrocortisone-supplemented medium eliminated the glucocorticoid-induced stimulation of Na-K ATPase activity. These results demonstrate a time- and dose-dependent effect of glucocorticoids on GR binding activity and a direct relation between this receptor-hormone interaction and Na-K ATPase activity in intact renal tubular epithelial cells.

Author List

Ellis D, Sothi TD, Avner ED

Author

Ellis D. Avner MD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cells, Cultured
Epithelial Cells
Estrenes
Hydrocortisone
Kidney Tubules
Mice
Mifepristone
Receptors, Glucocorticoid
Sodium-Potassium-Exchanging ATPase