CURRICULUM VITAE
Elizabeth Sweeny PhD
Elizabeth Sweeny PhD
Assistant Professor
Department of Biochemistry
Department of Biochemistry
OFFICE ADDRESS: |
Basic Science Building |
8701 Watertown Plank Rd |
Milwaukee, WI 53226 |
EDUCATION: |
09/2001 - 05/2005 Santa Barbara City College, Santa Barbara, CA |
09/2005 - 05/2007 BS, Loyola University Chicago, Chicago, IL |
09/2007 - 05/2014 PhD, University of Pennsylvania, Pennsylvania, PA |
POSTGRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS: |
05/2014 - 08/2014 Postdoctoral Fellow, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA |
09/2014 - 08/2015 Postdoctoral Fellow, MIT/Broad Institute Synthetic Biology Foundry, Cambridge, MA |
05/2016 - 11/2019 Postdoctoral Fellow, Department of Pathobiology/Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH |
11/2019 - 07/2021 Research Associate, Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH |
FACULTY APPOINTMENTS: |
08/2021 - Present Assistant Professor, Department of Biochemistry, The Medical College of Wisconsin, Milwaukee, WI |
AWARDS AND HONORS: |
2005 - 2007 Transfer scholarship, Loyola University Chicago |
2005 - 2007 Mulcahy Scholarship for Undergraduate Research, Loyola University Chicago |
2007 Fr. Morrissey Medal, Department of Chemistry, Loyola University Chicago |
2007 Graduated magna cum laude with Departmental Honors, Loyola University Chicago |
2009 A. Joshua Wand Poster Award, First Place, Department of Biochemistry and Biophysics, University of Pennsylvania |
2013 A. Joshua Wand Poster Award, First Place, Department of Biochemistry and Biophysics, University of Pennsylvania |
2017 Scientist of the Quarter, Department of Pathobiology, Lerner Research Institute, Cleveland Clinic |
2018 Travel Award, Nitric Oxide Society |
2019 William E. Lower Award - First prize, Non-clinical, Lerner Research Institute, Cleveland Clinic |
2019 Research Day Basic Science Poster Award, Lerner Research Institute, Cleveland Clinic |
MEMBERSHIPS IN HONORARY AND PROFESSIONAL SOCIETIES: |
2009 - 2014 University of Pennsylvania (Chemistry-Biology Interface Training Program Scholar) |
2014 - 2015 Women@Broad, Broad Institute (Member) |
2017 - 2019 Lerner Postdoctoral Association, Leadership Council, Lerner Research Institute (Member) |
2019 - Present American Heart Association (Member, Peer Reviewer for Fellowship Awards) |
RESEARCH GRANTS/AWARDS/CONTRACTS/PROJECTS: | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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PEER REVIEWED WORKSHOPS/PRESENTATIONS: |
International |
Elizabeth A. Sweeny, 9th International Conference on AAA Proteins. Kumamoto, Japan. November 2011. |
National |
Elizabeth A. Sweeny, Tetrapyrroles Gordon Research Conference. Newport, Rhode Island. July 2018. |
Local |
Elizabeth A. Sweeny, 29th Annual Biochemistry and Biophysics Retreat. Swarthmore, Pennsylvania. June 2012. |
EXTRAMURAL STUDENTS, FACULTY, RESIDENTS, AND CLINICAL/RESEARCH FELLOWS MENTORED: |
Undergraduate Students |
09/2009 - 06/2012 Beatrice Razzo, Research mentor, University of Pennsylvania |
09/2013 - 08/2014 Esin Gurpinar, Research mentor, University of Pennsylvania |
Medical Students |
06/2017 - 08/2017 Amulya Dega, Summer student research mentor, NEOMED |
BIBLIOGRAPHY |
Refereed Journal Publications/Original Papers |
1. Sweeny E.A., Hunt A.P., Batka A.E., Schlanger S, Lehnert N, Stuehr D.J. (2021) Nitric oxide and heme-NO stimulate superoxide production by NADPH oxidase 5. Free Radic Biol Med 172, 252-263. |
2. Sweeny E. A., Schlanger S, Stuehr D.J. (2020) Dynamic regulation of NADPH oxidase 5 by intracellular heme levels and cellular chaperones. Redox Biology 36. |
3. Dai, Y., Sweeny, E. A., Schlanger, S., Ghosh, A., and Stuehr, D. J. (2020) GAPDH delivers heme to soluble guanylyl cyclase. J Biol Chem 295(24), 8145-8154. |
4. Sweeny, E. A., Tariq, A., Gurpinar, E., Go, M. S., Sochor, M. A., Kan, Z. Y., Mayne, L., Englander, S. W., and Shorter, J. (2020) Structural and mechanistic insights into Hsp104 function revealed by synchrotron X-ray footprinting. J Biol Chem 295(6), 1517-1538. |
5. Tariq, A., Lin, J., Jackrel, M. E., Hesketh, C. D., Carman, P. J., Mack, K. L., Weitzman, R., Gambogi, C., Hernandez Murillo, O. A., Sweeny, E. A., Gurpinar, E., Yokom, A. L., Gates, S. N., Yee, K., Sudesh, S., Stillman, J., Rizo, A. N., Southworth, D. R. & Shorter, J. (2019). Mining Disaggregase Sequence Space to Safely Counter TDP-43, FUS, and α-Synuclein Proteotoxicity. Cell Rep 28, 2080-2095.e6. |
6. Durie, C. L., Lin, J., Scull, N. W., Mack, K. L., Jackrel, M. E., Sweeny, E. A., Castellano, L. M., Shorter, J. & Lucius, A. L. (2019). Hsp104 and Potentiated Variants Can Operate as Distinct Nonprocessive Translocases. Biophysical Journal 116, 1856-1872. |
7. Sweeny, E. A., Singh, A. B., Chakravarti, R., Martinez-Guzman, O., Saini, A., Haque, M. M., Garee, G., Dans, P. D., Hannibal, L., Reddi, A. R. & Stuehr, D. J. (2018). Glyceraldehyde-3-phosphate dehydrogenase is a chaperone that allocates labile heme in cells. J Biol Chem 293(37), 14557-14568. |
8. Guo, L., Kim, H. J., Wang, H., Monaghan, J., Freyermuth, F., Sung, J. C., O'Donovan, K., Fare, C. M., Diaz, Z., Singh, N., Zhang, Z. C., Coughlin, M., Sweeny, E. A., DeSantis, M. E., Jackrel, M. E., Rodell, C. B., Burdick, J. A., King, O. D., Gitler, A. D., Lagier-Tourenne, C., Pandey, U. B., Chook, Y. M., Taylor, J. P. & Shorter, J. (2018). Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains. Cell 173, 677-692 e20. |
9. Ghosh, A., Garee, G., Sweeny, E. A., Nakamura, Y. & Stuehr, D. J. (2018). Hsp90 chaperones hemoglobin maturation in erythroid and nonerythroid cells. Proceedings of the National Academy of Sciences, 201717993. |
10. Gates, S. N., Yokom, A. L., Lin, J., Jackrel, M. E., Rizo, A. N., Kendsersky, N. M., Buell, C. E., Sweeny, E. A., Mack, K. L., Chuang, E., Torrente, M. P., Su, M., Shorter, J. & Southworth, D. R. (2017). Ratchet-like polypeptide translocation mechanism of the AAA+ disaggregase Hsp104. Science 357, 273-279. |
11. Buren, S., Young, E. M., Sweeny, E. A., Lopez-Torrejon, G., Veldhuizen, M., Voigt, C. A. & Rubio, L. M. (2017). Formation of Nitrogenase NifDK Tetramers in the Mitochondria of Saccharomyces cerevisiae. ACS Synth Biol 6, 1043-1055. |
12. Weaver, C. L., Duran, E. C., Mack, K. L., Lin, J., Jackrel, M. E., Sweeny, E. A., Shorter, J. & Lucius, A. L. (2017). Avidity for Polypeptide Binding by Nucleotide-Bound Hsp104 Structures. Biochemistry 56, 2071-2075. |
13. Sweeny, E. A., Jackrel, M. E., Go, M. S., Sochor, M. A., Razzo, B. M., DeSantis, M. E., Gupta, K. & Shorter, J. (2015). The Hsp104 N-terminal domain enables disaggregase plasticity and potentiation. Mol Cell 57, 836-49. |
14. Desantis, M. E., Sweeny, E. A., Snead, D., Leung, E. H., Go, M. S., Gupta, K., Wendler, P. & Shorter, J. (2014). Conserved distal loop residues in the Hsp104 and ClpB middle domain contact nucleotide-binding domain 2 and enable Hsp70-dependent protein disaggregation. J Biol Chem 289, 848-67. |
15. DeSantis, M. E., Leung, E. H., Sweeny, E. A., Jackrel, M. E., Cushman-Nick, M., Neuhaus-Follini, A., Vashist, S., Sochor, M. A., Knight, M. N. & Shorter, J. (2012). Operational plasticity enables hsp104 to disaggregate diverse amyloid and nonamyloid clients. Cell 151, 778-93. |
16. Sweeny, E. A., DeSantis, M. E. & Shorter, J. (2011). Purification of hsp104, a protein disaggregase. J Vis Exp. |
17. Roberts, B. E., Duennwald, M. L., Wang, H., Chung, C., Lopreiato, N. P., Sweeny, E. A., Knight, M. N. & Shorter, J. (2009). A synergistic small-molecule combination directly eradicates diverse prion strain structures. Nat Chem Biol 5, 936-46. |
Books, Chapters, and Reviews |
1. Sweeny, E. A. & Shorter, J. (2016). Mechanistic and Structural Insights into the Prion-Disaggregase Activity of Hsp104. J Mol Biol 428, 1870-85. |
2. Sweeny, E. A. & Shorter, J. (2008). Prion proteostasis: Hsp104 meets its supporting cast. Prion 2, 135-40. |