Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

CURRICULUM VITAE

Elizabeth Sweeny PhD
Assistant Professor
Department of Biochemistry

OFFICE ADDRESS:
Basic Science Building
8701 Watertown Plank Rd
Milwaukee, WI 53226

EDUCATION:
09/2001 - 05/2005 Santa Barbara City College, Santa Barbara, CA
09/2005 - 05/2007 BS, Loyola University Chicago, Chicago, IL
09/2007 - 05/2014 PhD, University of Pennsylvania, Pennsylvania, PA

POSTGRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS:
05/2014 - 08/2014 Postdoctoral Fellow, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA
09/2014 - 08/2015 Postdoctoral Fellow, MIT/Broad Institute Synthetic Biology Foundry, Cambridge, MA
05/2016 - 11/2019 Postdoctoral Fellow, Department of Pathobiology/Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH
11/2019 - 07/2021 Research Associate, Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH

FACULTY APPOINTMENTS:
08/2021 - Present Assistant Professor, Department of Biochemistry, The Medical College of Wisconsin, Milwaukee, WI

AWARDS AND HONORS:
2005 - 2007 Transfer scholarship, Loyola University Chicago
2005 - 2007 Mulcahy Scholarship for Undergraduate Research, Loyola University Chicago
2007 Fr. Morrissey Medal, Department of Chemistry, Loyola University Chicago
2007 Graduated magna cum laude with Departmental Honors, Loyola University Chicago
2009 A. Joshua Wand Poster Award, First Place, Department of Biochemistry and Biophysics, University of Pennsylvania
2013 A. Joshua Wand Poster Award, First Place, Department of Biochemistry and Biophysics, University of Pennsylvania
2017 Scientist of the Quarter, Department of Pathobiology, Lerner Research Institute, Cleveland Clinic
2018 Travel Award, Nitric Oxide Society
2019 William E. Lower Award - First prize, Non-clinical, Lerner Research Institute, Cleveland Clinic
2019 Research Day Basic Science Poster Award, Lerner Research Institute, Cleveland Clinic

MEMBERSHIPS IN HONORARY AND PROFESSIONAL SOCIETIES:
2009 - 2014 University of Pennsylvania (Chemistry-Biology Interface Training Program Scholar)
2014 - 2015 Women@Broad, Broad Institute (Member)
2017 - 2019 Lerner Postdoctoral Association, Leadership Council, Lerner Research Institute (Member)
2019 - Present American Heart Association (Member, Peer Reviewer for Fellowship Awards)

RESEARCH GRANTS/AWARDS/CONTRACTS/PROJECTS:
Active
Peer Review
Title:
Heme allocation and disruptions in asthma and the failing heart
Source:
NIH
Role & Effort:
Principal Investigator
Dates:
08/01/2019 - 07/31/2024
Direct Funds:
$739,553 (total for all years)
  
Prior
Peer Review
Title:
Exploring Hsp104, a protein disaggregase, as a weapon against cardiac amyloidoses
Source:
AHA
Role & Effort:
Principal Investigator
Dates:
07/2010 - 06/2012
Direct Funds:
$60,000 (Predoctoral Fellowship)
  
Title:
Mechanisms governing heme insertion during maturation of the NADPH Oxidases
Source:
AHA
Role & Effort:
Principal Investigator
Dates:
07/2016 - 06/2018
Direct Funds:
$98,950 (Postdoctoral Fellowship)
  
Title:
Understanding the Heme regulatory network controlling NOX5 heme insertion and activity
Source:
AHA
Role & Effort:
Principal Investigator
Dates:
07/2018 - 06/2019
Direct Funds:
$54,316 (Postdoctoral Fellowship - one year)
  

PEER REVIEWED WORKSHOPS/PRESENTATIONS:
International
Elizabeth A. Sweeny, 9th International Conference on AAA Proteins. Kumamoto, Japan. November 2011.
 
National
Elizabeth A. Sweeny, Tetrapyrroles Gordon Research Conference. Newport, Rhode Island. July 2018.
 
Local
Elizabeth A. Sweeny, 29th Annual Biochemistry and Biophysics Retreat. Swarthmore, Pennsylvania. June 2012.
 

EXTRAMURAL STUDENTS, FACULTY, RESIDENTS, AND CLINICAL/RESEARCH FELLOWS MENTORED:
Undergraduate Students
09/2009 - 06/2012 Beatrice Razzo, Research mentor, University of Pennsylvania
09/2013 - 08/2014 Esin Gurpinar, Research mentor, University of Pennsylvania
 
Medical Students
06/2017 - 08/2017 Amulya Dega, Summer student research mentor, NEOMED
 


BIBLIOGRAPHY
Refereed Journal Publications/Original Papers
1. Sweeny E.A., Hunt A.P., Batka A.E., Schlanger S, Lehnert N, Stuehr D.J. (2021) Nitric oxide and heme-NO stimulate superoxide production by NADPH oxidase 5. Free Radic Biol Med 172, 252-263.
2. Sweeny E. A., Schlanger S, Stuehr D.J. (2020) Dynamic regulation of NADPH oxidase 5 by intracellular heme levels and cellular chaperones. Redox Biology 36.
3. Dai, Y., Sweeny, E. A., Schlanger, S., Ghosh, A., and Stuehr, D. J. (2020) GAPDH delivers heme to soluble guanylyl cyclase. J Biol Chem 295(24), 8145-8154.
4. Sweeny, E. A., Tariq, A., Gurpinar, E., Go, M. S., Sochor, M. A., Kan, Z. Y., Mayne, L., Englander, S. W., and Shorter, J. (2020) Structural and mechanistic insights into Hsp104 function revealed by synchrotron X-ray footprinting. J Biol Chem 295(6), 1517-1538.
5. Tariq, A., Lin, J., Jackrel, M. E., Hesketh, C. D., Carman, P. J., Mack, K. L., Weitzman, R., Gambogi, C., Hernandez Murillo, O. A., Sweeny, E. A., Gurpinar, E., Yokom, A. L., Gates, S. N., Yee, K., Sudesh, S., Stillman, J., Rizo, A. N., Southworth, D. R. & Shorter, J. (2019). Mining Disaggregase Sequence Space to Safely Counter TDP-43, FUS, and α-Synuclein Proteotoxicity. Cell Rep 28, 2080-2095.e6.
6. Durie, C. L., Lin, J., Scull, N. W., Mack, K. L., Jackrel, M. E., Sweeny, E. A., Castellano, L. M., Shorter, J. & Lucius, A. L. (2019). Hsp104 and Potentiated Variants Can Operate as Distinct Nonprocessive Translocases. Biophysical Journal 116, 1856-1872.
7. Sweeny, E. A., Singh, A. B., Chakravarti, R., Martinez-Guzman, O., Saini, A., Haque, M. M., Garee, G., Dans, P. D., Hannibal, L., Reddi, A. R. & Stuehr, D. J. (2018). Glyceraldehyde-3-phosphate dehydrogenase is a chaperone that allocates labile heme in cells. J Biol Chem 293(37), 14557-14568.
8. Guo, L., Kim, H. J., Wang, H., Monaghan, J., Freyermuth, F., Sung, J. C., O'Donovan, K., Fare, C. M., Diaz, Z., Singh, N., Zhang, Z. C., Coughlin, M., Sweeny, E. A., DeSantis, M. E., Jackrel, M. E., Rodell, C. B., Burdick, J. A., King, O. D., Gitler, A. D., Lagier-Tourenne, C., Pandey, U. B., Chook, Y. M., Taylor, J. P. & Shorter, J. (2018). Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains. Cell 173, 677-692 e20.
9. Ghosh, A., Garee, G., Sweeny, E. A., Nakamura, Y. & Stuehr, D. J. (2018). Hsp90 chaperones hemoglobin maturation in erythroid and nonerythroid cells. Proceedings of the National Academy of Sciences, 201717993.
10. Gates, S. N., Yokom, A. L., Lin, J., Jackrel, M. E., Rizo, A. N., Kendsersky, N. M., Buell, C. E., Sweeny, E. A., Mack, K. L., Chuang, E., Torrente, M. P., Su, M., Shorter, J. & Southworth, D. R. (2017). Ratchet-like polypeptide translocation mechanism of the AAA+ disaggregase Hsp104. Science 357, 273-279.
11. Buren, S., Young, E. M., Sweeny, E. A., Lopez-Torrejon, G., Veldhuizen, M., Voigt, C. A. & Rubio, L. M. (2017). Formation of Nitrogenase NifDK Tetramers in the Mitochondria of Saccharomyces cerevisiae. ACS Synth Biol 6, 1043-1055.
12. Weaver, C. L., Duran, E. C., Mack, K. L., Lin, J., Jackrel, M. E., Sweeny, E. A., Shorter, J. & Lucius, A. L. (2017). Avidity for Polypeptide Binding by Nucleotide-Bound Hsp104 Structures. Biochemistry 56, 2071-2075.
13. Sweeny, E. A., Jackrel, M. E., Go, M. S., Sochor, M. A., Razzo, B. M., DeSantis, M. E., Gupta, K. & Shorter, J. (2015). The Hsp104 N-terminal domain enables disaggregase plasticity and potentiation. Mol Cell 57, 836-49.
14. Desantis, M. E., Sweeny, E. A., Snead, D., Leung, E. H., Go, M. S., Gupta, K., Wendler, P. & Shorter, J. (2014). Conserved distal loop residues in the Hsp104 and ClpB middle domain contact nucleotide-binding domain 2 and enable Hsp70-dependent protein disaggregation. J Biol Chem 289, 848-67.
15. DeSantis, M. E., Leung, E. H., Sweeny, E. A., Jackrel, M. E., Cushman-Nick, M., Neuhaus-Follini, A., Vashist, S., Sochor, M. A., Knight, M. N. & Shorter, J. (2012). Operational plasticity enables hsp104 to disaggregate diverse amyloid and nonamyloid clients. Cell 151, 778-93.
16. Sweeny, E. A., DeSantis, M. E. & Shorter, J. (2011). Purification of hsp104, a protein disaggregase. J Vis Exp.
17. Roberts, B. E., Duennwald, M. L., Wang, H., Chung, C., Lopreiato, N. P., Sweeny, E. A., Knight, M. N. & Shorter, J. (2009). A synergistic small-molecule combination directly eradicates diverse prion strain structures. Nat Chem Biol 5, 936-46.
 
Books, Chapters, and Reviews
1. Sweeny, E. A. & Shorter, J. (2016). Mechanistic and Structural Insights into the Prion-Disaggregase Activity of Hsp104. J Mol Biol 428, 1870-85.
2. Sweeny, E. A. & Shorter, J. (2008). Prion proteostasis: Hsp104 meets its supporting cast. Prion 2, 135-40.