CURRICULUM VITAE
Jeannette M. Vasquez-Vivar PhD
Jeannette M. Vasquez-Vivar PhD
Professor
Department of Biophysics
Department of Biophysics
OFFICE ADDRESS: |
MACC Fund Research Center |
8701 Watertown Plank Rd |
Milwaukee, WI 53226 |
Phone: 414-955-8095 |
Fax: 414-955-6512 |
Email: jvvivar@mcw.edu |
EDUCATION: |
03/1980 - 12/1985 B.S., Biochemistry Universidad de Concepción, Chile |
03/1986 - 03/1988 Biochemistry, Universidad de Concepción, Chile |
06/1988 - 12/1992 Ph.D., Biochemistry, Universidade de São Paulo, Brazil |
POSTGRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS: |
01/1993 - 05/1996 Postdoctoral Fellow, Department of Biochemistry, Instituto de Química, Universidade de São Paulo, Brazil |
06/1996 - 07/1998 Research Fellow, Department of Pathology, Medical College of Wisconsin, Milwaukee, WI |
FACULTY APPOINTMENTS: |
03/1986 - 12/1987 Assistant Instructor, Biochemistry, Facultad de Ciencias Biologicas y Recursos Naturales, Universidad de Concepción, Chile |
02/1998 - 06/1998 Assistant Professor, Biochemistry, UNICAMP, São Paulo, Brazil (on leave) |
07/1998 - 06/2001 Visiting Assistant Professor, Pathology, Medical College of Wisconsin, Milwaukee, WI |
07/2001 - 06/2007 Assistant Professor, Biophysics, Medical College of Wisconsin, Milwaukee, WI |
07/2007 - 06/2014 Associate Professor, Biophysics, Medical College of Wisconsin, Milwaukee, WI |
2014 - Present Professor, Biophysics, Medical College of Wisconsin, Milwaukee, WI |
HOSPITAL AND CLINICAL ADMINISTRATIVE APPOINTMENTS: |
03/1988 - 07/1988 Central Laboratory, Hospital Barros Luco Trudeau, Chile, Head Serology Division, Santiago |
RESEARCH ADMINISTRATIVE APPOINTMENTS: |
1986 Expert Technician, Department of Industrias Forestales, Universidad del Bio-Bio |
2008 - Present Associate Director, Redox Biology Program, Medical College of Wisconsin, Milwaukee, WI |
AWARDS AND HONORS: |
1988 - 1990 Graduate Fellowship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil |
1990 - 1992 Graduate Fellowship, Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Brazil |
12/1992 - 06/1993 Postdoctoral Fellowship, Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Brazil |
07/1993 - 05/1996 Postdoctoral Fellowship, Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq), Brazil |
1994 - Present Travel Award, Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Brazil |
07/1997 - 05/1998 Postdoctoral Fellowship, American Heart Association-Wisconsin Affiliate |
07/26/2011 Lawrence Piette Lecture, 53th Rocky Mountain Conference Analytical Chemistry |
2012 Elsevier Top Reviewer, Free Radical Biology & Medicine |
2012 - 2013 Outstanding Medical Student Teacher, Medical College of Wisconsin |
2017 - 2018 Outstanding Medical Student Teacher, Medical College of Wisconsin |
2018 - 2019 Outstanding Educator, Graduate School, Medical College of Wisconsin |
MEMBERSHIPS IN HONORARY AND PROFESSIONAL SOCIETIES: |
1990 - 1996 Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq) |
1994 - Present Society for Free Radical Biology and Medicine |
1998 - 2001 Nitric Oxide |
1998 - 2003 American Heart Association- (AHA) Basic Science Council |
EDITORSHIPS/EDITORIAL BOARDS/JOURNAL REVIEWS: |
Editorship |
2011 - Present Editor, Frontiers in Oxidant Physiology |
2019 - Present Editor-in-Chief, Oxidative Medicine and Cellular Longevity (Hindawi Publisher) |
Editorial Board |
2004 - Present Free Radical Biology and Medicine |
2011 - 2019 Oxidative Medicine and Cellular Longevity |
LOCAL/REGIONAL APPOINTED LEADERSHIP AND COMMITTEE POSITIONS: |
2009 - 2018 Member, Institutional Review Board, Medical College of Wisconsin |
2013 - 2020 Member, Recruitment Committee for Interdisciplinary Doctoral Program in Biomedical Sciences (IDP), Graduate School Programs, Medical College of Wisconsin |
2020 - 2023 Member, School of Medicine Rank Committee, Medical College of Wisconsin |
NATIONAL ELECTED/APPOINTED LEADERSHIP AND COMMITTEE POSITIONS: |
2001 - 2002 Ad Hoc Reviewer, Alzheimer Association |
2002 Fonds zur Förderung der wissenschaftlichen Forschung (FWF), Austrian Science Fund |
2002 Dirección de Investigación y Postgrado, Universidad de Tarapaca, Arica, Chile |
2004 U.S. Civilian Research and Development Foundation Cooperative Grants Program |
2006 Ad Hoc Reviewer, Vascular Cell and Molecular Biology (VCMB) Study Section, National Institutes of Health (NIH) |
2007 - 2011 Regular Member, Vascular Cell and Molecular Biology (VCMB) Study Section, National Institutes of Health (NIH) |
2008 Ad Hoc Reviewer, Grant Agency, Academy of Sciences of the Czech Republic |
2008 Co-chaired "Nitric Oxide and Redox Sensitive Molecular Triggers", SFRBM 15th Annual Meeting, Indianapolis, IN |
2009 Co-chaired "Nitric Oxide and ROS in Hypoxia", SFRBM16th Annual Meeting, San Francisco, CA |
2010 Co-chaired "EPR and Spin Trapping in Redox Biology" session, EPR2010, San Juan, Puerto Rico |
2010 Co-chaired "Nitric Oxide and ROS in Vascular Biology, Orlando, FL |
2011 American Heart Association, Grant Reviewer National Center. |
2011 - Present Reviewer, Fonds zur Förderung der wissenschaftlichen Forschung (FWF), Austrian Science Fund |
2011 - Present Reviewer, Vascular Biology 3 Study section, American Heart Association, National Center |
2012 Reviewer, NHLBI Review Panel ZHL1 CSR-F (M1), National Institutes of Health, Center for Scientific Review (CSR) |
2012 Reviewer, NHLBI Review Panel ZHL1 CSR (F), National Institutes of Health, Center for Scientific Review (CSR) |
2013 Reviewer, NHLBI Review Panel ZHL1 CSR, National Institutes of Health, Center for Scientific Review (CSR) |
2014 Reviewer, NHLBI Review Panel ZHL1 CSR, National Institutes of Health, Center for Scientific Review (CSR) |
2015 Reviewer, NHLBI Review Panel ZHL1 CSR, National Institutes of Health, Center for Scientific Review (CSR) |
2017 Reviewer, NHLBI Review Panel ZHL1 CSR, National Institutes of Health, Center for Scientific Review (CSR) |
2018 Regular Member, Training Workforce Development (Committee C), Study Section, National Institute General Medical Sciences |
2019 Ad Hoc Reviewer, Neurological Sciences Training Initial Review Group 1 (NST-1) |
2020 Ad Hoc Reviewer, Neurological Sciences Training Initial Review Group 1 (NST-2) |
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INVITED LECTURES/WORKSHOPS/PRESENTATIONS: |
Local |
Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, WI, 1997 |
Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, WI, 1997 |
Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, WI, 1998 |
Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, 2006 |
Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI, 2006 |
Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI, 2007 |
Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI, 2008 |
Medicine, Cardiology Grand Rounds, Medical College of Wisconsin, Milwaukee, WI, 2011 |
Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI, 2011 |
Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI, 2016 |
Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI, 2017 |
Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, WI, 2019 |
National |
Ventura, Gordon Conference on Oxygen Radicals, Ventura, CA, 2000 |
Experimental Biology, San Diego, CA, 2000 |
Department of Pathology, University of Alabama at Birmingham, 2001 |
Denver, 43rd Rocky Mountain Conference on Analytical Chemistry, Denver, CO, 2001 |
12th International Symposium Chemistry and Biology of Pterins and Folates, Bethesda, MD, 2001 |
Department of Physiology, Louisiana University State Health Science Center, 2002 |
Department of Veterinary Medicine, University of Pennsylvania, 2002 |
Department of Pediatrics, Northwestern University, Evanston, IL, 2003 |
EPR 2005 Meeting, Columbus, OH, 2005 |
EPR 2007 Meeting, Chicago, IL, 2007 |
Department of Pathobiology, Cleveland Clinic, Cleveland, OH, 2008 |
Department of Medicine, University of Illinois at Chicago, Chicago, IL, 2008 |
Department of Endocrinology and Diabetes, Oklahoma University Health Sciences Center, Oklahoma City, OK, 2008 |
Department of Pathology, LSUHSC, Shreveport, LA, 2010 |
Department of Medicine, UIC, Chicago, IL, 2010 |
Gordon Conference on Oxygen Radicals, Ventura, CA, 2010 |
Department of Cardiology-Medicine, University of Illinois at Chicago, Chicago, IL, 2011 |
Department of Neonatology, Wayne University, Detroit, MI, 2017 |
International Workshop on EPR in Biology and Medicine, Kraków, Poland, 2019 |
International |
Instituto Nacional de Câncer, Rio de Janeiro, Brazil, 1992 |
Universidad Católica de Valparaiso, Chile, 1993 |
7th Biennial Meeting of the Society for Free Radical Research International, Sydney, Australia, 1994 |
UNICAMP, Universidade Estadual de Campinas, São Paulo, Brazil, 1994 |
XIVReunião Anual da SBBq., Caxambú, Brazil, 1995 |
6th International Symposium on Spin-trapping, Marseilles, France, 2000 |
10th Biennial Meeting of the Society for Free Radical Research International, Kyoto, 10/2001 |
5th Workshop on EPR Applications in Biology and Medicine, Kraków, Poland, 2002 |
6th Workshop on EPR Applications in Biology and Medicine, Kraków, Poland, 2004 |
Free Radicals in Montevideo 2007, Montevideo, 2007 |
EPR2010, San Juan, Puerto Rico, 05/2010 |
First Adriatic Symposium on Biophysical Approaches in Biomedical Studies, Split, Croatia, 2014 |
IXth International Workshop on EPR in Biology and Medicine, Kraków, Poland, 2016 |
Xth International Workshop on EPR in Biology and Medicine, Kraków, Poland, 2017 |
Second Adriatic Symposium on Biophysical Approaches in Biomedical Studies, Split, Croatia, 2017 |
XIth International Workshop on EPR in Biology and Medicine, Kraków, Poland, 2019 |
PEER REVIEWED WORKSHOPS/PRESENTATIONS: |
National |
VÁSQUEZ-VIVAR, J., Altered BH4 metabolism in hypercholesterolemic rabbits, Scientifc Session, American Heart Association, Anaheim, CA, 2001 |
VÁSQUEZ-VIVAR J., Contrasting expression of GTP cyclohydrolase-I and tetrahydrobiopterin levels in LPS-treated endothelial cells and cardiac myocytes, Scientific Session, American Heart Association, Chicago, IL, 2002 |
VÁSQUEZ-VIVAR, J., Hypercholesterolemia alters vascular response to sepiapterin, 13th International Conference on Pterins, Maui, HI, 03/2003 |
VÁSQUEZ-VIVAR, J., Detection of superoxide with DMPO and improved nitrones. Rigorous Detection and Identification of Free Radicals in Biology and Medicine Workshop, Society for Free Radical Biology and Medicine (SFRBM) 12th Annual Meeting, Austin, TX, 2005 |
VÁSQUEZ-VIVAR, J., Detection of intracellular superoxide with hydroethidine and MitosoxTM. Free Radic Biol Med. 39: Suppl 1:S115, SFRBM 12th Annual Meeting, Austin, TX, 2005 |
VÁSQUEZ-VIVAR, J., Application of Mito-DEPMPO and Mito-SOX in superoxide measurements from uncoupled nitric oxide synthase, Free Radic Biol Med. 40: Suppl 1:S103, SFRBM 13th Annual Meeting, Denver, CO, 2006 |
VÁSQUEZ-VIVAR J., Neuroprotective actions of tetrahydrobiopterin against hypoxia-induced motor impairments in the fetal brain, International Conference on Tetrahydrobiopterin, Phenylketonuria and Nitric Oxide Synthase., St. Moritz, Switzerland, 2008 |
VASQUEZ-VIVAR, J., Redox regulation of nitric oxide signaling and critical methodologies. Critical Methods for Redox Biology: from Test Tube to the Clinic Workshop, SFRBM 16th Annual Meeting, San Francisco, CA, 2009 |
VÁSQUEZ-VIVAR J., Quantitative Relationship Between NADPH Depletion and Inhibition of NOX2-Induced Superoxide Radical Anion, SFRBM 23rd Annual Meeting, San Francisco, CA, 2016 |
International |
VASQUEZ-VIVAR, J., Induction of iNOS but not BH4 in cytokine and LPS-stimulated adult cardiomyocytes, International Conference on Tetrahydrobiopterin, Phenylketonutria and Nitric Oxide Synthase, St. Moritz, Switzerland, 2008 |
COMMITTEE SERVICE: |
Medical College of Wisconsin |
2003 - 2004 Member, Graduate School Curriculum and Programs Committee, Medical College of Wisconsin |
2013 - Present Member, Recruitment Committee for Interdisciplinary Graduate Program, Medical College of Wisconsin |
2018 Member, Robert D. and Patricia E. Kern Institute for the Transformation of Medical Education Director Search Committee, Medical College of Wisconsin |
MEDICAL COLLEGE TEACHING ACTIVITIES: |
Graduate Student Education |
1999 Taught “Detection of Free Radicals by Spin Resonance Spectroscopy” |
2002 Taught “Detection of Free Radicals in Biological Systems” |
2005 Organized the EPR Journal Club during the winter semesters |
2006 Taught graduate course on “Free Radicals in Biology” |
2008 Taught graduate course on “Free Radicals in Biology” |
2009 Organized Journal Club, Biophysics graduate program |
2012 - 2019 Facilitator Physician Scientist M2/M3 Small Groups |
2019 - 2020 Organizer of Biophysics Graduate Seminar Series |
EXTRAMURAL TEACHING: |
Graduate Student Education |
2018 Anna University Chennai, Global Initiative for Academic Networks (GIAN), Chennai, India, Taught course titled “Cell Redox Biology and Therapeutics” (25h lectures, 1 week) |
MCW STUDENTS, FACULTY, RESIDENTS AND CLINICAL/RESEARCH FELLOWS MENTORED: |
Undergraduate Students |
Zhen Ding, Medical College of Wisconsin, 2011 - 2013 Thesis Committee |
Ching-Fang Chang, Medical College of Wisconsin, 2011 - 2013 Thesis Committee |
Medical Students |
Michael Larson, Medical College of Wisconsin, 2012 - Present MD/PhD Thesis Committee member |
Eric Chow, Medical College of Wisconsin, Mentor-Research Clinician Pathway |
Graduate Students |
Jessica Weber, Medical College of Wisconsin, 2006 |
Naiara Herrera Araujo, Brazilian Exchange Student, Medical College of Wisconsin, 2018 - 2019 |
PhD Committees |
Nick Kettenhofen, Medical College of Wisconsin, 2008 |
Mariam Hartley, Medical College of Wisconsin, 2009 |
Timothy Flewelen, Medical College of Wisconsin, 2010 |
Postdoctoral Students |
Savitha Sethumadhavan, Medical College of Wisconsin, 2010 - 2012 Mentor-AHA Postdoctoral Award |
Joanna Celinska, Medical College of Wisconsin, 2010 - 2011 Mentor |
Karthikeyan Thirugnanam, Medical College of Wisconsin, 2015 - 2018 |
Savitha Stehumadhavan, Medical College of Wisconsin, Mentor - 3rd year AHA Postdoctoral Award |
EXTRAMURAL STUDENTS, FACULTY, RESIDENTS, AND CLINICAL/RESEARCH FELLOWS MENTORED: |
Undergraduate Students |
Alexander Kislia, Milwaukee Scholl of Engineering), 2004 Mentor |
Kevin Kreitzman, Milwaukee Scholl of Engineering, 2005 Preceptor |
Ben Droese, U. Wisconsin-La Crosse, 2005 |
Ricardo Villareal, Cornell University, 2009 Preceptor |
Maritza Martin, Marquete University, 2010 - 2011 Preceptor |
Abdel Reyes, Rochester University, 2011 - 2012 Preceptor |
Mathew Weber, University of Georgia, 2014 Preceptor |
Graduate Students |
PhD Committees |
Everett Tate, University of Wisconsin-Milwaukee, 2015 - 2019 |
PROGRAMMATIC DEVELOPMENTS: |
Research Programs |
Redox Biology Program, Associate Director |
BIBLIOGRAPHY |
Refereed Journal Publications/Original Papers |
1. Vasquez-Vivar J, Augusto O. ESR detection of free radical intermediates during autoxidation of 5-hydroxyprimaquine. Free Radic Res Commun. 1990;9(3-6):383-9. |
2. Vásquez-Vivar J, Augusto O. Hydroxylated metabolites of the antimalarial drug primaquine. Oxidation and redox cycling. J Biol Chem. 1992 Apr 05;267(10):6848-54. |
3. Escobar JA, Vásquez-Vivar J, Cilento G. Free radicals and excited species in the metabolism of indole-3-acetic acid and its ethyl ester by horseradish peroxidase and by neutrophils. Photochem Photobiol. 1992 Jun;55(6):895-902. |
4. Escobar, J.A., VÁSQUEZ-VIVAR, J., Cilento, G. Oxidation of Indole-3-acetic acid by sonnicated neutrophils. Pitfalls in correlating EPR signals and chemiluminescence. Photochem. Photobiol. 59:130-134, 1994. |
5. Vásquez-Vivar J, Augusto O. Oxidative activity of primaquine metabolites on rat erythrocytes in vitro and in vivo. Biochem Pharmacol. 1994 Jan 20;47(2):309-16. |
6. Augusto O, Radi R, Gatti RM, Vásquez-Vivar J. Detection of secondary radicals from peroxynitrite-medicated oxidations by electron spin resonance. Methods Enzymol. 1996;269:346-54. |
7. Vásquez-Vivar J, Santos AM, Junqueira VB, Augusto O. Peroxynitrite-mediated formation of free radicals in human plasma: EPR detection of ascorbyl, albumin-thiyl and uric acid-derived free radicals. Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3):869-76. PMCID: PMC1217137 |
8. Vásquez-Vivar J, Hogg N, Pritchard KA Jr, Martasek P, Kalyanaraman B. Superoxide anion formation from lucigenin: an electron spin resonance spin-trapping study. FEBS Lett. 1997 Feb 17;403(2):127-30. |
9. Vásquez-Vivar J, Denicola A, Radi R, Augusto O. Peroxynitrite-mediated decarboxylation of pyruvate to both carbon dioxide and carbon dioxide radical anion. Chem Res Toxicol. 1997 Jul;10(7):786-94. |
10. Vásquez-Vivar J, Martasek P, Hogg N, Masters BS, Pritchard KA Jr, Kalyanaraman B. Endothelial nitric oxide synthase-dependent superoxide generation from adriamycin. Biochemistry. 1997 Sep 23;36(38):11293-7. |
11. Vásquez-Vivar J, Kalyanaraman B, Martásek P, Hogg N, Masters BS, Karoui H, Tordo P, Pritchard KA Jr. Superoxide generation by endothelial nitric oxide synthase: the influence of cofactors. Proc Natl Acad Sci U S A. 1998 Aug 04;95(16):9220-5. PMCID: PMC21319 |
12. Gatti RM, Alvarez B, Vasquez-Vivar J, Radi R, Augusto O. Formation of spin trap adducts during the decomposition of peroxynitrite. Arch Biochem Biophys. 1998 Jan 01;349(1):36-46. |
13. VÁSQUEZ-VIVAR, J., Kalyanaraman, B., Martásek, P., Hogg, N., Masters, B.S., Karoui, H., Tordo, P., Pritchard, K.A. Jr. Superoxide generation from endothelial nitric oxide synthase. The role of cofactors. Proc. Natl. Acad. Sci. U.S.A. 95:9220-9225, 1998. |
14. Vásquez-Vivar J, Martásek P, Hogg N, Karoui H, Masters BS, Pritchard KA Jr, Kalyanaraman B. Electron spin resonance spin-trapping detection of superoxide generated by neuronal nitric oxide synthase. Methods Enzymol. 1999;301:169-77. |
15. Vásquez-Vivar J, Hogg N, Martásek P, Karoui H, Tordo P, Pritchard KA Jr, Kalyanaraman B. Effect of redox-active drugs on superoxide generation from nitric oxide synthases: biological and toxicological implications. Free Radic Res. 1999 Dec;31(6):607-17. |
16. Vásquez-Vivar J, Hogg N, Martásek P, Karoui H, Pritchard KA Jr, Kalyanaraman B. Tetrahydrobiopterin-dependent inhibition of superoxide generation from neuronal nitric oxide synthase. J Biol Chem. 1999 Sep 17;274(38):26736-42. |
17. VÁSQUEZ-VIVAR, J., Joseph, J., Karoui, H., Zhang, H., Miller, J., Martásek, P. EPR spin trapping of superoxide from nitric oxide synthase. Analusis 28:487-492, 2000. |
18. Vasquez-Vivar J, Kalyanaraman B, Kennedy MC. Mitochondrial aconitase is a source of hydroxyl radical. An electron spin resonance investigation. J Biol Chem. 2000 May 12;275(19):14064-9. |
19. Zhang H, Joseph J, Vasquez-Vivar J, Karoui H, Nsanzumuhire C, Martásek P, Tordo P, Kalyanaraman B. Detection of superoxide anion using an isotopically labeled nitrone spin trap: potential biological applications. FEBS Lett. 2000 May 04;473(1):58-62. |
20. Vásquez-Vivar J, Kalyanaraman B. Generation of superoxide from nitric oxide synthase. FEBS Lett. 2000 Sep 22;481(3):305-6. |
21. Vásquez-Vivar J, Whitsett J, Martásek P, Hogg N, Kalyanaraman B. Reaction of tetrahydrobiopterin with superoxide: EPR-kinetic analysis and characterization of the pteridine radical. Free Radic Biol Med. 2001 Oct 15;31(8):975-85. |
22. Vásquez-Vivar J, Martásek P, Whitsett J, Joseph J, Kalyanaraman B. The ratio between tetrahydrobiopterin and oxidized tetrahydrobiopterin analogues controls superoxide release from endothelial nitric oxide synthase: an EPR spin trapping study. Biochem J. 2002 Mar 15;362(Pt 3):733-9. PMCID: PMC1222439 |
23. VÁSQUEZ-VIVAR, J., Duquaine, D., Whitsett, J., Kalyanaraman, B., Joseph, J., Rajagopalan, S. Altered tetrahydrobiopterin (BH4) metabolism in atherosclerosis: implications for the use of oxidized BH4 analogs and thiol antioxidants. Arterioscler. Thromb. Vasc. Biol. 22:1655-1661, 2002. |
24. Vásquez-Vivar J, Duquaine D, Whitsett J, Kalyanaraman B, Rajagopalan S. Altered tetrahydrobiopterin metabolism in atherosclerosis: implications for use of oxidized tetrahydrobiopterin analogues and thiol antioxidants. Arterioscler Thromb Vasc Biol. 2002 Oct 01;22(10):1655-61. |
25. Zhao H, Kalivendi S, Zhang H, Joseph J, Nithipatikom K, Vásquez-Vivar J, Kalyanaraman B. Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: potential implications in intracellular fluorescence detection of superoxide. Free Radic Biol Med. 2003 Jun 01;34(11):1359-68. |
26. Duquaine, D., Hirsch, G.A., Chakrabarti, A., Han, Z., Kehrer, C., Brook, R., Joseph, J., Schott, A., Kalyanaraman, B., VÁSQUEZ-VIVAR, J., Rajagopalan, S. Rapid onset endothelial dysfunction with adriamycin: Evidence for a dysfunctional nitric oxide synthase. Vasc. Med. 8:101-107, 2003. |
27. Vásquez-Vivar J, Kalyanaraman B, Martásek P. The role of tetrahydrobiopterin in superoxide generation from eNOS: enzymology and physiological implications. Free Radic Res. 2003 Feb;37(2):121-7. |
28. Duquaine D, Hirsch GA, Chakrabarti A, Han Z, Kehrer C, Brook R, Joseph J, Schott A, Kalyanaraman B, Vasquez-Vivar J, Rajagopalan S. Rapid-onset endothelial dysfunction with adriamycin: evidence for a dysfunctional nitric oxide synthase. Vasc Med. 2003 May;8(2):101-7. |
29. Kalivendi S, Hatakeyama K, Whitsett J, Konorev E, Kalyanaraman B, Vásquez-Vivar J. Changes in tetrahydrobiopterin levels in endothelial cells and adult cardiomyocytes induced by LPS and hydrogen peroxide--a role for GFRP? Free Radic Biol Med. 2005 Feb 15;38(4):481-91. |
30. Zhao H, Joseph J, Fales HM, Sokoloski EA, Levine RL, Vasquez-Vivar J, Kalyanaraman B. Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence. Proc Natl Acad Sci U S A. 2005 Apr 19;102(16):5727-32. PMCID: PMC556312 |
31. Porter R, Jáchymová M, Martásek P, Kalyanaraman B, Vásquez-Vivar J. Reductive activation of Cr(Vi) by nitric oxide synthase. Chem Res Toxicol. 2005 May;18(5):834-43. |
32. Pieper GM, Nilakantan V, Halligan NL, Khanna AK, Hilton G, Vásquez-Vivar J. Nitric oxide formation in acutely rejecting cardiac allografts correlates with GTP cyclohydrolase I activity. Biochem J. 2005 Nov 01;391(Pt 3):541-7. PMCID: PMC1276954 |
33. Whitsett J, Martásek P, Zhao H, Schauer DW, Hatakeyama K, Kalyanaraman B, Vásquez-Vivar J. Endothelial cell superoxide anion radical generation is not dependent on endothelial nitric oxide synthase-serine 1179 phosphorylation and endothelial nitric oxide synthase dimer/monomer distribution. Free Radic Biol Med. 2006 Jun 01;40(11):2056-68. |
34. Zielonka J, Vasquez-Vivar J, Kalyanaraman B. The confounding effects of light, sonication, and Mn(III)TBAP on quantitation of superoxide using hydroethidine. Free Radic Biol Med. 2006 Oct 01;41(7):1050-7. |
35. Robinson KM, Janes MS, Pehar M, Monette JS, Ross MF, Hagen TM, Murphy MP, Beckman JS. Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proc Natl Acad Sci U S A. 2006 Oct 10;103(41):15038-43. PMCID: PMC1586181 |
36. Hardy M, Rockenbauer A, Vásquez-Vivar J, Felix C, Lopez M, Srinivasan S, Avadhani N, Tordo P, Kalyanaraman B. Detection, characterization, and decay kinetics of ROS and thiyl adducts of mito-DEPMPO spin trap. Chem Res Toxicol. 2007 Jul;20(7):1053-60. PMCID: PMC4455887 |
37. Whitsett J, Picklo MJ Sr, Vasquez-Vivar J. 4-Hydroxy-2-nonenal increases superoxide anion radical in endothelial cells via stimulated GTP cyclohydrolase proteasomal degradation. Arterioscler Thromb Vasc Biol. 2007 Nov;27(11):2340-7. |
38. Singh U, Devaraj S, Vasquez-Vivar J, Jialal I. C-reactive protein decreases endothelial nitric oxide synthase activity via uncoupling. J Mol Cell Cardiol. 2007 Dec;43(6):780-91. PMCID: PMC2771555 |
39. Zielonka J, Srinivasan S, Hardy M, Ouari O, Lopez M, Vasquez-Vivar J, Avadhani NG, Kalyanaraman B. Cytochrome c-mediated oxidation of hydroethidine and mito-hydroethidine in mitochondria: identification of homo- and heterodimers. Free Radic Biol Med. 2008 Mar 01;44(5):835-46. PMCID: PMC2692199 |
40. Zielonka J, Vasquez-Vivar J, Kalyanaraman B. Detection of 2-hydroxyethidium in cellular systems: a unique marker product of superoxide and hydroethidine. Nat Protoc. 2008;3(1):8-21. |
41. Vásquez-Vivar J, Whitsett J, Ionova I, Konorev E, Zielonka J, Kalyanaraman B, Shi Y, Pieper GM. Cytokines and lipopolysaccharides induce inducible nitric oxide synthase but not enzyme activity in adult rat cardiomyocytes. Free Radic Biol Med. 2008 Oct 01;45(7):994-1001. PMCID: PMC2578873 |
42. Ionova IA, Vásquez-Vivar J, Whitsett J, Herrnreiter A, Medhora M, Cooley BC, Pieper GM. Deficient BH4 production via de novo and salvage pathways regulates NO responses to cytokines in adult cardiac myocytes. Am J Physiol Heart Circ Physiol. 2008 Nov;295(5):H2178-87. PMCID: PMC2614582 |
43. Ji H, Tan S, Igarashi J, Li H, Derrick M, Martásek P, Roman LJ, Vásquez-Vivar J, Poulos TL, Silverman RB. Selective neuronal nitric oxide synthase inhibitors and the prevention of cerebral palsy. Ann Neurol. 2009 Feb;65(2):209-17. PMCID: PMC2658647 |
44. Pieper GM, Ionova IA, Cooley BC, Migrino RQ, Khanna AK, Whitsett J, Vásquez-Vivar J. Sepiapterin decreases acute rejection and apoptosis in cardiac transplants independently of changes in nitric oxide and inducible nitric-oxide synthase dimerization. J Pharmacol Exp Ther. 2009 Jun;329(3):890-9. PMCID: PMC2683770 |
45. Vásquez-Vivar J, Whitsett J, Derrick M, Ji X, Yu L, Tan S. Tetrahydrobiopterin in the prevention of hypertonia in hypoxic fetal brain. Ann Neurol. 2009 Sep;66(3):323-31. PMCID: PMC2785106 |
46. Hein TW, Singh U, Vasquez-Vivar J, Devaraj S, Kuo L, Jialal I. Human C-reactive protein induces endothelial dysfunction and uncoupling of eNOS in vivo. Atherosclerosis. 2009 Sep;206(1):61-8. PMCID: PMC2735606 |
47. Vásquez-Vivar J. Tetrahydrobiopterin, superoxide, and vascular dysfunction. Free Radic Biol Med. 2009 Oct 15;47(8):1108-19. PMCID: PMC2852262 |
48. Rabbani ZN, Spasojevic I, Zhang X, Moeller BJ, Haberle S, Vasquez-Vivar J, Dewhirst MW, Vujaskovic Z, Batinic-Haberle I. Antiangiogenic action of redox-modulating Mn(III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin, MnTE-2-PyP(5+), via suppression of oxidative stress in a mouse model of breast tumor. Free Radic Biol Med. 2009 Oct 01;47(7):992-1004. PMCID: PMC2749298 |
49. Du J, Wei N, Xu H, Ge Y, Vásquez-Vivar J, Guan T, Oldham KT, Pritchard KA Jr, Shi Y. Identification and functional characterization of phosphorylation sites on GTP cyclohydrolase I. Arterioscler Thromb Vasc Biol. 2009 Dec;29(12):2161-8. PMCID: PMC2798731 |
50. Yu L, Derrick M, Ji H, Silverman RB, Whitsett J, Vásquez-Vivar J, Tan S. Neuronal nitric oxide synthase inhibition prevents cerebral palsy following hypoxia-ischemia in fetal rabbits: comparison between JI-8 and 7-nitroindazole. Dev Neurosci. 2011;33(3-4):312-9. PMCID: PMC3225251 |
51. Ionova IA, Vásquez-Vivar J, Cooley BC, Khanna AK, Whitsett J, Herrnreiter A, Migrino RQ, Ge ZD, Regner KR, Channon KM, Alp NJ, Pieper GM. Cardiac myocyte-specific overexpression of human GTP cyclohydrolase I protects against acute cardiac allograft rejection. Am J Physiol Heart Circ Physiol. 2010 Jul;299(1):H88-96. PMCID: PMC2904123 |
52. Ge ZD, Ionova IA, Vladic N, Pravdic D, Hirata N, Vásquez-Vivar J, Pratt PF Jr, Warltier DC, Pieper GM, Kersten JR. Cardiac-specific overexpression of GTP cyclohydrolase 1 restores ischaemic preconditioning during hyperglycaemia. Cardiovasc Res. 2011 Jul 15;91(2):340-9. PMCID: PMC3125073 |
53. Leskov IL, Whitsett J, Vasquez-Vivar J, Stokes KY. NAD(P)H oxidase and eNOS play differential roles in cytomegalovirus infection-induced microvascular dysfunction. Free Radic Biol Med. 2011 Dec 15;51(12):2300-8. PMCID: PMC3272703 |
54. Belik J, McIntyre BA, Enomoto M, Pan J, Grasemann H, Vasquez-Vivar J. Pulmonary hypertension in the newborn GTP cyclohydrolase I-deficient mouse. Free Radic Biol Med. 2011 Dec 15;51(12):2227-33. PMCID: PMC5050525 |
55. Wang J, Alexanian A, Ying R, Kizhakekuttu TJ, Dharmashankar K, VÁSQUEZ-VIVAR, J., Gutterman DD, Widlansky ME. Acute exposure to low glucose rapidly induces endothelial dysfunction and mitochondrial oxidative stress: role for AMP kinase. Arterioscler Thromb Vasc Biol. 32:712-20. 2012. |
56. Mao M, Sudhahar V, Ansenberger-Fricano K, Fernandes DC, Tanaka LY, Fukai T, Laurindo FR, Mason RP, Vasquez-Vivar J, Minshall RD, Stadler K, Bonini MG. Nitroglycerin drives endothelial nitric oxide synthase activation via the phosphatidylinositol 3-kinase/protein kinase B pathway. Free Radic Biol Med. 2012 Jan 15;52(2):427-35. PMCID: PMC3432314 |
57. Drobyshevsky A, Luo K, Derrick M, Yu L, Du H, Prasad PV, Vasquez-Vivar J, Batinic-Haberle I, Tan S. Motor deficits are triggered by reperfusion-reoxygenation injury as diagnosed by MRI and by a mechanism involving oxidants. J Neurosci. 2012 Apr 18;32(16):5500-9. PMCID: PMC3332550 |
58. Sethumadhavan S, Vasquez-Vivar J, Migrino RQ, Harmann L, Jacob HJ, Lazar J. Mitochondrial DNA variant for complex I reveals a role in diabetic cardiac remodeling. J Biol Chem. 2012 Jun 22;287(26):22174-82. PMCID: PMC3381178 |
59. Whitsett J, Rangel Filho A, Sethumadhavan S, Celinska J, Widlansky M, Vasquez-Vivar J. Human endothelial dihydrofolate reductase low activity limits vascular tetrahydrobiopterin recycling. Free Radic Biol Med. 2013 Oct;63:143-50. PMCID: PMC3748942 |
60. Yu L, Vásquez-Vivar J, Jiang R, Luo K, Derrick M, Tan S. Developmental susceptibility of neurons to transient tetrahydrobiopterin insufficiency and antenatal hypoxia-ischemia in fetal rabbits. Free Radic Biol Med. 2014 Feb;67:426-36. PMCID: PMC3945116 |
61. Hardy M, Poulhés F, Rizzato E, Rockenbauer A, Banaszak K, Karoui H, Lopez M, Zielonka J, Vasquez-Vivar J, Sethumadhavan S, Kalyanaraman B, Tordo P, Ouari O. Mitochondria-targeted spin traps: synthesis, superoxide spin trapping, and mitochondrial uptake. Chem Res Toxicol. 2014 Jul 21;27(7):1155-65. PMCID: PMC5452977 |
62. Drobyshevsky A, Takada SH, Luo K, Derrick M, Yu L, Quinlan KA, Vasquez-Vivar J, Nogueira MI, Tan S. Elevated spinal monoamine neurotransmitters after antenatal hypoxia-ischemia in rabbit cerebral palsy model. J Neurochem. 2015 Feb;132(4):394-402. PMCID: PMC4329027 |
63. Lakshmikanthan S, Zheng X, Nishijima Y, Sobczak M, Szabo A, Vasquez-Vivar J, Zhang DX, Chrzanowska-Wodnicka M. Rap1 promotes endothelial mechanosensing complex formation, NO release and normal endothelial function. EMBO Rep. 2015 May;16(5):628-37. PMCID: PMC4428051 |
64. Mainali L, Vasquez-Vivar J, Hyde JS, Subczynski WK. Spin-labeled small unilamellar vesicles with the <i>T</i><sub>1</sub>-sensitive saturation-recovery EPR display as an oxygen sensitive analyte for measurement of cellular respiration. Appl Magn Reson. 2015 Aug 01;46(8):885-895. PMCID: PMC4591545 |
65. Baotic I, Weihrauch D, Procknow J, Vasquez-Vivar J, Ge ZD, Sudhakaran S, Warltier DC, Kersten JR. Isoflurane favorably modulates guanosine triphosphate cyclohydrolase-1 and endothelial nitric oxide synthase during myocardial ischemia and reperfusion injury in rats. Anesthesiology. 2015 Sep;123(3):582-9. PMCID: PMC4543441 |
66. Sethumadhavan S, Whitsett J, Bennett B, Ionova IA, Pieper GM, Vasquez-Vivar J. Increasing tetrahydrobiopterin in cardiomyocytes adversely affects cardiac redox state and mitochondrial function independently of changes in NO production. Free Radic Biol Med. 2016 Apr;93:1-11. PMCID: PMC5498285 |
67. Yang M, Cooley BC, Li W, Chen Y, Vasquez-Vivar J, Scoggins NO, Cameron SJ, Morrell CN, Silverstein RL. Platelet CD36 promotes thrombosis by activating redox sensor ERK5 in hyperlipidemic conditions. Blood. 2017 May 25;129(21):2917-2927. PMCID: PMC5445574 |
68. Liu Y, Baumgardt SL, Fang J, Shi Y, Qiao S, Bosnjak ZJ, Vásquez-Vivar J, Xia Z, Warltier DC, Kersten JR, Ge ZD. Transgenic overexpression of GTP cyclohydrolase 1 in cardiomyocytes ameliorates post-infarction cardiac remodeling. Sci Rep. 2017 Jun 08;7(1):3093. PMCID: PMC5465102 |
69. Zielonka J, Joseph J, Sikora A, Hardy M, Ouari O, Vasquez-Vivar J, Cheng G, Lopez M, Kalyanaraman B. Mitochondria-Targeted Triphenylphosphonium-Based Compounds: Syntheses, Mechanisms of Action, and Therapeutic and Diagnostic Applications. Chem Rev. 2017 Aug 09;117(15):10043-10120. PMCID: PMC5611849 |
70. Hardy M, Zielonka J, Karoui H, Sikora A, Michalski R, Podsiadły R, Lopez M, Vasquez-Vivar J, Kalyanaraman B, Ouari O. Detection and Characterization of Reactive Oxygen and Nitrogen Species in Biological Systems by Monitoring Species-Specific Products. Antioxid Redox Signal. 2018 May 20;28(15):1416-1432. PMCID: PMC5910052 |
71. Vasquez-Vivar J, Shi Z, Luo K, Thirugnanam K, Tan S. Tetrahydrobiopterin in antenatal brain hypoxia-ischemia-induced motor impairments and cerebral palsy. Redox Biol. 2017 Oct;13:594-599. PMCID: PMC5554922 |
72. Stuehr DJ, Vasquez-Vivar J. Nitric oxide synthases-from genes to function. Nitric Oxide. 2017 Feb 28;63:29. PMCID: PMC5495577 |
73. Sánchez-Aranguren LC, Espinosa-González CT, González-Ortiz LM, Sanabria-Barrera SM, Riaño-Medina CE, Nuñez AF, Ahmed A, Vasquez-Vivar J, López M. Soluble Fms-Like Tyrosine Kinase-1 Alters Cellular Metabolism and Mitochondrial Bioenergetics in Preeclampsia. Front Physiol. 2018;9:83. PMCID: PMC5845757 |
74. Antholine WE, Vasquez-Vivar J, Quirk BJ, Whelan HT, Wu PK, Park JI, Myers CR. Treatment of Cells and Tissues with Chromate Maximizes Mitochondrial 2Fe2S EPR Signals. Int J Mol Sci. 2019 Mar 06;20(5). PMCID: PMC6429069 |
75. Shi Z, Vasquez-Vivar J, Luo K, Yan Y, Northington F, Mehrmohammadi M, Tan S. Ascending Lipopolysaccharide-Induced Intrauterine Inflammation in Near-Term Rabbits Leading to Newborn Neurobehavioral Deficits. Dev Neurosci. 2018;40(5-6):534-546. PMCID: PMC9873358 |
76. Vasquez-Vivar J, Shi Z, Jeong JW, Luo K, Sharma A, Thirugnanam K, Tan S. Neuronal vulnerability to fetal hypoxia-reoxygenation injury and motor deficit development relies on regional brain tetrahydrobiopterin levels. Redox Biol. 2020 Jan;29:101407. PMCID: PMC6928344 |
77. Herrera NA, Duchatsch F, Kahlke A, Amaral SL, Vasquez-Vivar J. In vivo vascular rarefaction and hypertension induced by dexamethasone are related to phosphatase PTP1B activation not endothelial metabolic changes. Free Radic Biol Med. 2020 May 20;152:689-696. PMCID: PMC8546799 |
78. Zhao H, Joseph J, Fales HM, Sokoloski EA, Levine RL, Vasquez-Vivar J, Kalyanaraman B. Erratum: Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence (Proceedings of the National Academy of Sciences of the United States of America (April 19, 2005) 102, 16 (5727-5732)) Proceedings of the National Academy of Sciences of the United States of America. 21 June 2005;102(25):9086. |
79. Vasquez-Vivar J, Joseph J, Karoui H, Zhang H, Miller J, Martasek P. EPR spin trapping of superoxide from nitric oxide synthase Analusis. Jul;28(6):487-492. |
80. Escobar JA, Vásquez‐Vivar J, Cilento G. OXIDATION OF INDOLE‐3‐ACETIC ACID BY SONICATED NEUTROPHILS. PITFALLS IN CORRELATING EPR SIGNALS AND CHEMILUMINESCENCE Photochemistry and Photobiology. January 1994;59(1):130-134. |
81. Singh B, Kosuru R, Lakshmikanthan S, Sorci-Thomas MG, Zhang DX, Sparapani R, Vasquez-Vivar J, Chrzanowska M. Endothelial Rap1 (Ras-Association Proximate 1) Restricts Inflammatory Signaling to Protect From the Progression of Atherosclerosis. Arterioscler Thromb Vasc Biol. 2021 Feb;41(2):638-650. PMCID: PMC8105264 |
82. Kosuru R, Singh B, Lakshmikanthan S, Nishijima Y, Vasquez-Vivar J, Zhang DX, Chrzanowska M. Distinct Signaling Functions of Rap1 Isoforms in NO Release From Endothelium. Front Cell Dev Biol. 2021;9:687598. PMCID: PMC8247587 |
83. Kosuru R, Singh B, Lakshmikanthan S, Nishijima Y, Vasquez-Vivar J, Zhang DX, Chrzanowska M. Corrigendum: Distinct Signaling Functions of Rap1 Isoforms in NO Release From Endothelium. Front Cell Dev Biol. 2021;9:741935. PMCID: PMC8378398 |
84. Vasquez-Vivar J, Shi Z, Tan S. Tetrahydrobiopterin in Cell Function and Death Mechanisms. Antioxid Redox Signal. 2022 Jul;37(1-3):171-183. PMCID: PMC9293684 |
Books, Chapters, and Reviews |
1. VÁSQUEZ-VIVAR, J., Augusto, O. Formation of hydrogen peroxide from primaquine metabolites. In Oxygen Paradox in Biology & Medicine. Davies, K.A., Ed. CLEUP Press, Padova, 1995, pp 157-167. |
2. Jones, C.L., VÁSQUEZ-VIVAR, J., Griscavage, J., Masters, B.S.S., Martásek, P., Kalyanaraman, B., Gross, S.S. Only fully-reduced pterins attenuate superoxide production by eNOS: an explanation for the tetrahydrobiopterin requirement? In Biology of Nitric Oxide (Part 7). Moncada, S., Gustafsson, L., Wiklund, P., and Higgs, E.A., Eds. Portland Press, London, 2000, pp 118. |
3. VÁSQUEZ-VIVAR, J., Hogg, N. Martásek, P., Masters, B.S.S., Karoui, H., Pritchard, K.A. Jr., Kalyanaraman, B. Tetrahydrobiopterin inhibits superoxide generation by neuronal nitric oxide synthase. In Biology of Nitric Oxide (Part 7). Moncada, S., Gustafsson, L., Wiklund, P., and Higgs, E.A., Eds. Portland Press, London, 2000, pp 4. |
4. Kalyanaraman, B., VÁSQUEZ-VIVAR, J., Hogg, N., Joseph, J., Zhang, H., Karoui, H., Martásek, P., Tordo, P. Detection of superoxide formation from nitric oxide synthases using electron spin resonance: Spin-trapping technique. In Free Radicals in Chemistry, Biology and Medicine. Yoshikawa, T., Toyohuni, S., Yamamoto, Y. and Naito, Y., Eds. OICA International, London, 2000, pp 115-120. |
5. Jones, C.L., VÁSQUEZ-VIVAR, J., Kalyanaraman, B., Griscavage-Ennis, J.M., Gross, S.S. The redox status of bound pterin cofactor determines whether eNOS produces no or superoxide anion: [3H]-BH4 binding studies provide insights into vascular pathophysiology. In Proceedings Book from the 12th International Symposium on the Chemistry and Biology of Pteridines and Folates. Milstein, S., Kapatos, G., Levine, R.A. and Shane, B., Eds. Kluwer Academic Publishers, Boston, 2001, pp 271-276. |
6. VÁSQUEZ-VIVAR, J., Kalyanaraman B., Martásek P. The role of tetrahydrobiopterin in superoxide generation from eNOS: enzymology and physiological implications (review). Free Radic. Res. 37:121-127, 2003. |
7. VÁSQUEZ-VIVAR, J., Martásek, P., Kalyanaraman, B. Superoxide generation from nitric oxide synthase - Role of cofactors and protein-protein interaction. In: Biomedical EPR; PART A: Free Radicals, Metals Medicine and Physiology. Biological Magnetic Resonance, Vol. 24. Eaton, S.S., Eaton, G.R. and Berliner, L.J., Eds. Kluwer Academic Publishers, Boston, 2004, pp 75-91. |
Editorials, Letters to Editor, Other |
1. VÁSQUEZ-VIVAR, J., Kalyanaraman, B. Generation of superoxide from nitric oxide synthase. FEBS Lett. 481:305-306, 2000. |
2. VÁSQUEZ-VIVAR, J., Rajagopalan, S. Possible impact of tetrahydrobiopterin and sepiapterin on endothelial dysfunction (In response) Arterioscler. Thromb. Vasc. Biol. 23:913, 2003. |
Non-Refereed Journal Publications/Original Papers |
1. Augusto, O., Hix, S., Morais, M.S., VÁSQUEZ-VIVAR, J. Free radical reactions: formation of adducts with biomolecules and their biological significance. Ciência e Cultura 47:279-288, 1995. |
