CURRICULUM VITAE
Sally S. Twining PhD
Sally S. Twining PhD
Assistant Dean, Professor
Department of Biochemistry
Department of Biochemistry
OFFICE ADDRESS: |
Medical Education Building |
8701 Watertown Plank Rd |
Milwaukee, WI 53226 |
EDUCATION: |
09/1965 - 08/1969 B.S. in Chemistry and Music, Bowling Green State University, Bowling Green, OH |
09/1969 - 06/1971 M.A. in Chemistry, Bowling Green State University, Bowling Green, OH |
09/1973 - 12/1976 Ph.D. in Physiological Chemistry, Ohio State University, Columbus, OH |
POSTGRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS: |
12/1976 - 12/1979 Mayo Fellow in Immunology, Mayo Clinic Foundation, Rochester, MN |
05/1979 - 08/1980 VFW Auxiliary Fellow in Biochemistry, Medical College of Wisconsin, Milwaukee, WI |
FACULTY APPOINTMENTS: |
09/1971 - 06/1973 Instructor in Chemistry, Bowling Green State University, Bowling Green, OH |
09/1980 - 06/1983 Instructor in Biochemistry, Medical College of Wisconsin, Milwaukee, WI |
07/1983 - 06/1989 Assistant Professor in Biochemistry, Medical College of Wisconsin, Milwaukee, WI |
07/1985 - 06/1989 Assistant Professor in Ophthalmology (Secondary), Medical College of Wisconsin, Milwaukee, WI |
07/1989 - 2004 Associate Professor in Biochemistry, Medical College of Wisconsin, Milwaukee, WI |
07/1989 - 2004 Associate Professor in Ophthalmology (Secondary), Medical College of Wisconsin, Milwaukee, WI |
09/1998 - 03/1999 Visiting Scientist in Biochemistry, University of Wisconsin, Madison, WI |
09/2004 - Present Professor in Ophthalmology (secondary), Medical College of Wisconsin, Milwaukee, WI |
09/2004 - Present Professor in Biochemistry with tenure, Medical College of Wisconsin, Milwaukee, WI |
EDUCATIONAL ADMINISTRATIVE APPOINTMENTS: |
01/1990 - Present Director of the Glucose Laboratory in Medical Biochemistry, Medical College of Wisconsin, Milwaukee, WI |
09/1991 - Present Block Leader, Medical Biochemistry, Medical College of Wisconsin, Milwaukee, WI |
01/1993 - 1999 Course Director, Contemporary Biochemistry (Graduate), Medical College of Wisconsin, Milwaukee, WI |
01/2008 - 05/2008 Course Director, Introduction to Biostatistics (Graduate), Medical College of Wisconsin, Milwaukee, WI |
09/2008 - Present Course Director, Boundaries of Science and Medical Practice (Graduate), Medical College of Wisconsin, Milwaukee, WI |
08/2009 - Present Director, PhD in Basic and Translational Sciences (Graduate), Medical College of Wisconsin, Milwaukee, WI |
09/2010 - Present Co-Module Director, GI-Nutrition, Pilot Innovation Curriculum, Medical College of Wisconsin, Milwaukee, WI |
03/2011 - Present Executive Integration Director, Pilot Innovation Curriculum, Medical College of Wisconsin, Milwaukee, WI |
AWARDS AND HONORS: |
05/1983 Travel Fellowship, Association for Research in Vision and Ophthalmology |
08/1985 Travel Fellowship, American Society of Biological Chemists 13th International Congress of Biochemistry, Amsterdam |
08/1991 Travel Fellowship, American Society of Biological Chemists 15th International Congress of Biochemistry, Israel |
05/1992 Alumna of the Year, Bowling Green State University, Chemistry Department |
06/1996 Innovative Educational Project Award, Department of Family and Community Medicine, Medical College Of Wisconsin |
09/2001 Twenty-Year Faculty Award, Medical College of Wisconsin |
09/2006 Twenty-five-Year Faculty Award, Medical College of Wisconsin |
2007 - 2008 Outstanding Medical Student Teacher, Medical College of Wisconsin |
05/2008 Distinguished Service Award, Medical College of Wisconsin |
09/2008 Graduate Mentor of the Year, Medical College of Wisconsin |
2008 - 2009 Outstanding Medical Student Teacher, Medical College of Wisconsin |
2009 - 2010 Outstanding Medical Student Teacher, Medical College of Wisconsin |
2010 Fellow, The Association for Research in Vision and Ophthalmology |
MEMBERSHIPS IN HONORARY AND PROFESSIONAL SOCIETIES: |
1969 - Present American Chemical Society |
1973 - Present American Association for the Advancement of Science |
1974 - Present Sigma Xi |
1982 - Present Association for Research in Vision and Ophthalmology |
1984 - Present American Society for Biochemistry and Molecular Biology |
2000 - Present American Society for Cell Biology |
EDITORSHIPS/EDITORIAL BOARDS/JOURNAL REVIEWS: |
Editorial Board |
1998 - 2008 Investigative Ophthalmology and Visual Sciences for Proteases in the Eye and Biochemistry of the Cornea |
Journal Review |
1982 - Present Alcoholism |
1982 - Present Molecular Vision |
1982 - Present British Journal of Ophthalmology |
1982 - Present Experimental Eye Research |
1982 - Present Protein Expression and Purification |
1982 - Present Alcohol |
1982 - Present Biological Bulletin |
1982 - Present Journal of Biological Chemistry |
1982 - Present Archives of Ophthalmology |
1982 - Present FASEB Journal |
1982 - Present Biochemical Journal |
1982 - Present Current Eye Research |
1982 - Present Investigative Ophthalmology and Visual Sciences |
1982 - Present Biochemistry |
1982 - Present Biochimica Biophysica Acta |
1982 - Present Analytical Biochemistry |
1982 - Present BioTechniques |
NATIONAL ELECTED/APPOINTED LEADERSHIP AND COMMITTEE POSITIONS: |
1985 Ad Hoc Member, Physiological Chemistry Study Section, NIH |
1988 Reviewer, Veterans Administration Merit Review |
1989 Ad Hoc Reviewer, USDA |
1991 - 1993 Ad Hoc Member, Visual Sciences C Study Section, NIH |
1993 Ad Hoc Reviewer, USDA |
1994 - 1998 Member, Visual Sciences A Study Section, NIH |
1997 Member, Peer Review Panel on Photorefractive Keratectomy (PRK) Research, American Institute of Biological Sciences |
1998 Ad Hoc Member, Scientific Board of Councilors, National Eye Institute, NIH |
2001 Member, Visual Sciences Special Study Section, NIH |
2003 - 2006 Member, Program Committee, Association for Research in Vision and Ophthalmology |
2005 - 2006 Co-Chair, Cornea Program Committee, Association for Research in Vision and Ophthalmology |
2005 Ad Hoc Member, Fogarty Grants, NIH Study Section |
2006 Member, Chemical Counterterrism, Special NIH Study Section |
2007 Chair, Anterior Eye Disease Special Study Section, NIH |
2007 - Present Chair, External Advisory Board, UMDNJ/Rutgers University CounterACT Research Center of Excellence, AR055073 |
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INVITED LECTURES/WORKSHOPS/PRESENTATIONS: |
International |
Proteases and Proteoglycans in Vitamin A Deficiency, World Congress on the Cornea, III, Boston, 1987 |
Corneal ulceration: Disruption of the Proteinase-Inhibitor Balance, XII International Congress on Eye Research, Yokohama, Japan, 09/1996 |
Corneal Ulceration and the Proteinase-Inhibitor Balance, International Contact Lens Society, Florence, Italy, 08/1997 |
Proteinases in Corneal Ulceration, International Congress on Eye Research, Paris, France, 08/1999 |
Structure-function of Maspin, SERPINS Meeting, Cairns, Australia, 06/2005 |
Corneal Proteinases, ICER Meeting, Buenos Aires, 10/2006 |
National |
Human Corneal Serine Proteinases, National Meeting of the Association for Research in Vision and Ophthalmology, Ft. Lauderdale, FL, 05/1997 |
Pseudomonas aeruginosa Proteases, National Meeting of the Association for Research in Vision and Ophthalmology, Ft. Lauderdale, FL, 05/1998 |
Effects of maspin on corneal stromal cell gene expression: Increased protein levels of SPARC and PAI-1, National Meeting of the Association for Research in Vision and Ophthalmology, Ft. Lauderdale, FL, 04/2004 |
Role of the reactive site loop in the function of maspin, Steinbock Symposium, University of Wisconsin, 05/2004 |
Local |
Vitamin A and Immunity, Fourteenth Symposium on Clinical Nutrition, Medical College of Wisconsin, Milwaukee, WI, 09/1994 |
PEER REVIEWED WORKSHOPS/PRESENTATIONS: |
National |
Mechanism of Pepsinogen Activation, Bowling Green State University, Department of Chemistry, Bowling Green, OH, 03/1984 |
Role of Women in Chemistry, Bowling Green State University, American Chemical Society Student Affiliate, Bowling Green, OH, 03/1984 |
Proteases and Protease Inhibitors in the Cornea, Boston Eye Research Institute, Boston, MA, 03/1987 |
Role of Proteinase Inhibitors in the Cornea, National Institutes of Health, Eye Institute, Bethesda, MD, 10/1991 |
Isolation and Properties of Corneal Proteoglycan-Proteinase Complexes, Bowling Green State University, Department of Chemistry, Bowling Green, OH, 01/1992 |
Role of Proteinases in Pseudomonas Keratitis, Vistacon, Jacksonville, FL, 03/1993 |
Corneal 1-Proteinase Inhibitor: Specific Control by Retinoids, National Institutes of Health, Eye Institute, Bethesda, MD, 10/1995 |
Plasminogen in the Cornea, Temple University, Department of Biology, Philadelphia, PA, 04/1998 |
Chameleon Proteins: Mechanism of Conversion of Plasminogen to Angiostatin, Bowling Green State University, Department of Chemistry, Bowling Green, OH, 02/2001 |
Molecular Mechanisms By Which The Cornea Prevents Tumor Formation, Roswell Park Cancer Institute, Buffalo, NY, 04/2001 |
Conversion of Serpins: Making Ovalbumin into a Maspin-like Molecule, Jackson State University, Jackson, MS, 02/2002 |
Conversion of Serpins: Making Ovalbumin into a Maspin-like Molecule, National Institutes of Health, National Eye Institute, Bethesda, MD, 02/2002 |
Regional |
Proteases in Vitamin A Deficient Corneas, College of St. Catherine, Department of Chemistry, St. Paul, MN, 11/1983 |
Proteases and Protease Inhibitors in Normal Corneas, Hope College, Department of Chemistry, Holland, MI, 11/1985 |
Role of Polymorphonuclear Leukocytes in Pseudomonas aeruginosa Infections, Wayne State University, Department of Anatomy and Cell Biology, Detroit, MI, 03/1989 |
Effects of Polymorphonuclear Leukocytes on the Cornea during Inflammation, Northern Illinois University, Department of Biology, DeKalb, IL, 05/1990 |
Control of Proteolysis in the Cornea, University of Illinois Medical School, Dept. Ophthalmology, Chicago, IL, 04/1995 |
Pseudomonas Proteinases: A New Elastase Variant, Wayne State University, Dept. Microbiology, Detroit, MI, 12/1997 |
Proteinases and Inhibitors in the Cornea, Northern Illinois University, Center for Biochemical and Biophysical Studies, DeKalb, IL, 03/2002 |
Corneal Wound Healing, Hillsdale College, Department of Chemistry, Hillsdale, MI, 09/2003 |
Local |
Proteases in Corneal Ulceration Due to Vitamin A Deficiency with Histological Correlations, University of Wisconsin Parkside, Department of Life Sciences, Kenosha, WI, 10/1984 |
The Influence of the Immune System in Pseudomonas Keratitis in Rats, Carroll College, Department of Biology, Waukesha, WI, 10/1986 |
Opportunities for Research in Medicine, University of Wisconsin Milwaukee, Milwaukee, WI, 11/1987 |
Effect of Vitamin A Deficiency on Polymorphonuclear Leukocyte Function, Beloit College, Department of Biology, Beloit, WI, 02/1988 |
Perspectives on Research, Target MD Seminar, University of Wisconsin - Milwaukee, Milwaukee, WI, 12/1988 |
Research in Medicine, Target MD Seminar, University of Wisconsin - Milwaukee, Milwaukee, WI, 10/1989 |
Effect of Vitamin A Deficiency on Polymorphonuclear Leukocyte Function, U. Wisconsin-Madison, Department of Nutrition, Madison, WI, 11/1992 |
COMMITTEE SERVICE: |
Medical College of Wisconsin |
1984 - 1986 Admission Committee (Elected), Subcommittee for Minority Admissions, Medical College of Wisconsin |
1985 - 1987 Member, Target M.D., Committee of the University of Wisconsin Milwaukee, Medical College of Wisconsin |
1986 - 1987 Chair, Admission Committee (Elected), Admission Subcommittee on Recruitment, Medical College of Wisconsin |
1986 - 1987 Student Affairs Committee (Appointed), Medical College of Wisconsin |
1988 - 2004 Library Committee, Medical College of Wisconsin |
1989 Dean's Committee for the Internal Review, Physiology Department, Medical College of Wisconsin |
1990 - 1993 Marquette Scholars Committee, Medical College of Wisconsin, Faculty Representative, Admission Committee (Elected), Medical College of Wisconsin |
1990 - 1993 Animal Care Committee (Elected), Medical College of Wisconsin |
1991 - 1993 Dissertation Prize Committee, Medical College of Wisconsin |
1991 - 2006 Animal Surgery Core Director, NEI Core Grant for Vision Research Committee, Medical College of Wisconsin |
1991 - 1995 Graduate Studies Committee, Medical College of Wisconsin |
1991 - 1993 Graduate Recruitment Director, Graduate Committee, Medical College of Wisconsin |
1993 - 1996 Academic Standing Committee (Elected), Medical College of Wisconsin |
1993 - 1995 Faculty Welfare Committee (Elected), Medical College of Wisconsin |
1993 - 1995 Armand J. Quick Award Committee, Medical College of Wisconsin |
1993 - 1996 1993-1996 Integrated Academic Information Management Systems Planning Committee, Medical College of Wisconsin |
1993 - 1995 Chair, Nominating Committee, Medical College of Wisconsin |
1993 - 1995 Graduate Program Director, Graduate Committee, Medical College of Wisconsin |
1993 - 1998 Representative, Bloodborne Pathogen, Medical College of Wisconsin |
1994 Chair of the Graduate School Review, Liaison Committee for Medical Education Review Committee, Medical College of Wisconsin |
1996 - 1997 Search Committee for Chair of Ophthalmology, Medical College of Wisconsin |
1996 Search Committee for Associate Dean for Curricular Affairs, Medical College of Wisconsin |
1998 - 2001 Chair, Armand J. Quick Award Committee, Medical College of Wisconsin |
1999 - 2004 Computer Committee, Medical College Of Wisconsin |
1999 - 2002 Admission Committee (Appointed), Medical College of Wisconsin |
2000 - 2002 Admission Committee (Appointed), Executive Committee of the Admission Committee, Medical College of Wisconsin |
2000 - 2002 Instructional Computing Committee, Medical College of Wisconsin |
2000 - 2005 Chair, Student Welfare Committee, Medical College of Wisconsin |
2000 - Present Ph.D. Qualifying Exam Committees, Medical College of Wisconsin |
2000 - 2002 Admission Committee (Appointed), Committee on Diversity, Medical College of Wisconsin |
2001 - 2003 Graduate Committee, Medical College Of Wisconsin |
2001 - Present Mentoring Committee, Medical College of Wisconsin |
2001 - Present Graduate Studies Committee, Medical College of Wisconsin |
2001 Focus Group on the Name, Identity and Image of the Medical College of Wisconsin, Medical College of Wisconsin |
2002 - 2003 LCME Accreditation Task Force, Medical College of Wisconsin |
2002 - 2005 Curriculum and Evaluation Committee (Elected), Executive Committee, Medical College of Wisconsin |
2002 - 2007 Women’s Faculty Council (Appointed), Medical College of Wisconsin |
2002 - 2003 LCME Accreditation Review Committee on Students, Medical College of Wisconsin |
2002 - 2005 Chair, Curriculum and Evaluation Committee (Elected), M1/M2 Subcommittee, Medical College of Wisconsin |
2003 Focus Group for the Establishment of Guidelines for the MCW Educational IRB, Medical College of Wisconsin |
2003 Search Committee for Associate Dean for Curricular Affairs, Medical College of Wisconsin |
2003 - 2006 Graduate Program Director, Graduate Committee, Medical College Of Wisconsin |
2003 - 2004 Chair, Women’s Faculty Council (Appointed), Program Committee, Medical College of Wisconsin |
2003 Focus Group for the Revision of Medical Information Management Course, Medical College of Wisconsin |
2004 - 2005 Chair Elect, Women’s Faculty Council (Appointed), Medical College of Wisconsin |
2004 Focus Group for Establishment of the Global Learning Objectives for M1-M2 years, Medical College of Wisconsin |
2004 - 2006 M1 Advisor, Medical School Student Assembly, Medical College of Wisconsin |
2005 - 2006 Women’s Faculty Council Representative, Faculty Council, Medical College of Wisconsin |
2005 - 2006 Chair, Women’s Faculty Council (Appointed), Medical College of Wisconsin |
2006 - 2010 Executive Committee, Medical College of Wisconsin |
2006 - 2010 Armand J. Quick Award Committee, Medical College Of Wisconsin |
2006 - 2012 Faculty Council and Governance Committee (Elected), Medical College of Wisconsin |
2006 - Present Women in Science Advisory Committee, Medical College of Wisconsin |
2006 - 2010 Curriculum Committee, Medical College of Wisconsin |
2006 - 2007 Chair Emeritus, Women’s Faculty Council (Appointed), Medical College of Wisconsin |
2006 - 2007 Vice President, Faculty Council and Governance Committee (Elected), Medical College of Wisconsin |
2006 - Present Biochemistry Co-Core Director, NEI Core Grant for Vision Research Committee, Medical College of Wisconsin |
2007 Co-Leader for Education and Training, CTSA Key Function Group, Medical College of Wisconsin |
2007 Chair, Women’s Faculty Council (Appointed), Woman in Science Award Selection Committee, Medical College of Wisconsin |
2007 - 2009 Faculty Council Representative, Executive Committee of the Faculty, Medical College of Wisconsin |
2007 - 2008 Chair, Ad Hoc Certificate Committee, Medical College of Wisconsin |
2007 - 2008 Search Committee for Cancer Faculty, Medical College of Wisconsin |
2007 - Present Co-Leader for Education and Training, CTSA Key Function Group, Medical College Of Wisconsin |
2007 - 2008 Sr Vice President, Faculty Council and Governance Committee (Elected), Medical College of Wisconsin |
2008 - 2009 President, Faculty Council and Governance Committee (Elected), Medical College of Wisconsin |
2008 - Present Faculty and Staff Gift Drive Committee (Appointed), Medical College of Wisconsin |
2009 - Present Ad Hoc Member Representing the Faculty Governance Committee (Appointed), Curriculum and Evaluation Committee, Medical College of Wisconsin |
2009 - 2012 Past President, Faculty Council and Governance Committee (Elected), Medical College of Wisconsin |
2009 - 2010 Graduate School Dean Search Committee, Medical College of Wisconsin |
2009 - 2011 Dean’s Educational Innovation Advisory Committee, Medical College of Wisconsin |
2009 - 2010 Search Committee for the President of MCW, Medical College of Wisconsin |
2009 - 2011 Researcher Search Committee, Medical College of Wisconsin |
2010 LCME Accreditation Task Force, Medical College of Wisconsin |
2010 hair, LCME Accreditation Review Committee on Faculty, Medical College of Wisconsin |
2010 - Present MS in Clinical and Translational Science Advisory Committee, Medical College Of Wisconsin |
2011 Search Committee for the Senior Vice President for Finance and Administration of MCW, Medical College of Wisconsin |
MCW TEACHING ACTIVITIES: |
Medical Student Education |
1983 - Present Medical Biochemistry |
1983 - 1987 Summer Bridging Program: Lecturer |
1985 - 1988 Introduction to Clinical Medicine |
2002 - Present Basic Science of Pediatrics Problem Solving – M4 Selective |
2003 Grand Rounds-Intrauterine Growth Restriction |
2011 Pilot Innovation Curriculum |
Graduate Student Education |
1984 - 1987 Seminar in Biochemistry |
1985 - 1999 Protein Chemistry |
1988 - 2000 Enzyme Chemistry |
1990 - Present Physical Properties of Macromolecules-Biophysical Techniques in Biochemistry |
1990 - 2000 Lipids, Carbohydrates and Membranes |
1993 - 2005 Contemporary Topics in Biochemistry, Course Director (Every 3 yrs) |
1999 - Present Biochemistry of the Cell |
2003 - Present Protein Chemistry |
2006 - 2008 Advanced Cell Signaling, Lecture on Protease Activated Receptors |
2007 - Present Enzyme Kinetics |
2008 Introduction to Biostatistics, Lectures on Applications of Biostatistics |
2008 - Present Boundaries of Science and Medical Practice |
2009 - Present Introduction to Clinical and Translational Sciences |
Resident and Fellow Education |
1987 - Present Ophthalmology Resident Lecture Series |
Community/Lay Public |
2003 Mini Med School-Biochemical Basis of Phenylketonuria |
MCW STUDENTS, FACULTY, RESIDENTS AND CLINICAL/RESEARCH FELLOWS MENTORED: |
Medical Students |
1988 Jeff Jahnke, Medical College of Wisconsin |
1989 Jerry Loushin, Medical College of Wisconsin |
1990 Jerry Loushin, Medical College of Wisconsin |
1994 Simon Wright, Medical College of Wisconsin |
1994 Jerry Loushin, Medical College of Wisconsin |
1995 Vannah Nguyen, Medical College of Wisconsin |
Graduate Students |
PhD Committees |
1982 - 1985 Joyce Tucker, Serine Proteases of Aspergillus fumigatus, Medical College of Wisconsin |
1985 - 1988 Tracy Kangas, Effect of Edema on Corneal Proteoglycans, Medical College of Wisconsin |
1989 - 1995 Kevin Langford, Isolation and Characterization of the High Molecular Weight, Medical College of Wisconsin |
1991 - 1996 Goran Boskovic, Control of Synthesis of 1-Proteinase Inhibitor in the Human Cornea, Medical College of Wisconsin |
1995 - 2000 Monica Olague-Alcala, Characterization of the Structural Alterations Caused by a Disease-Associated Mutation (G627V) in the BP180 Protein (Type XVII Collagen), Medical College of Wisconsin |
1997 - 2002 Chatri Ngamkitdaichakul, Regulation of Corneal Cells by Maspin, Medical College of Wisconsin |
2001 - 2005 Guangjie Sun, Understanding the Molecular Basis Underlying Carbohydrate Recognition by the CD-MPR, Medical College of Wisconsin |
2005 - Present Malathi Narayan, Phosphorylation of Maspin Synthesized by the Corneal Epithelium, Medical College of Wisconsin |
2006 - 2010 Mark White, Role of PepD in Tuberculosis, Medical College of Wisconsin |
2006 - 2010 Erin Hassemer, Anatomy and Neurosciences, Characterization of the woe mutation in ADAM 17, Medical College of Wisconsin |
2007 - Present Kate Merath, Ocular mutation in SHEFB-P, Medical College of Wisconsin |
2007 - 2010 Mariam Hartley, Roles of Metals and Metal Ligands in the Active Site of Vibrio proteolyticus aminopeptidase, Medical College of Wisconsin |
2007 - 2009 Hong Guan, Palmitoyl Acyl Transferases in Breast Cancer, Medical College of Wisconsin |
2007 - 2010 Kay Ishizuka, Spatial regulation of Ras protein and DHHC palmitoyltransferases in Sacharomyces cerevisiae, Medical College of Wisconsin |
2008 - 2011 Alicia, Castonguay, Function of Retinoid Binding on CD-MPR, Medical College of Wisconsin |
2008 - Present Susan Salti, Role of Granzyme B in T-Helper Cell Function, Medical College of Wisconsin |
2008 - Present Emily Andreae, Role of Thrombin in the Cornea, Medical College of Wisconsin |
2008 - Present Bethany Volkman, PITX in Lens Development, Medical College of Wisconsin |
2009 - Present Luis Paniagua, Role of RAP-1 in Platelets, Medical College of Wisconsin |
2009 - Present Elizabeth Ntantie, Phosphorylation of SmgGDS, Medical College of Wisconsin |
2010 - Present LiLi Du, Characterization of allosteric enhancers for the A3 adenosine receptor, Medical College of Wisconsin |
2011 - Present Maxime Heroux, Proteomics of Proteus Syndrome, Medical College of Wisconsin |
MS Committees |
1984 - 1987 Renee Madden, Studies on the Interaction of Plasminogen Activator Inhibitor Type One with Thrombin: Possible Regulatory Mechanism of Fibrinolysis, Medical College of Wisconsin |
1996 - 1999 Karen Tiffany, Structure of Isovaleryl Acyl Dehydrogenase, Medical College of Wisconsin |
1996 - 1998 Mark Nickolas, Gene Structure of Corneal Maspin, Medical College of Wisconsin |
2004 - 2005 Kelly Soules, Characterization of Ocular Mutants in Zebrafish, Medical College of Wisconsin |
2004 - 2005 Adam Harder, Structure Function of BRAK, Medical College of Wisconsin |
PhD Students Advised |
1991 - 1996 Goran Boskovic, Control of Synthesis of 1-Proteinase Inhibitor in the Human Cornea, Medical College of Wisconsin |
1997 - 2002 Chatri Ngamikitdaichakul, Regulation of Corneal Cells by Maspin, Medical College of Wisconsin |
2005 - 2010 Malathi Narayan, Mechanism of Maspin Function, Medical College of Wisconsin |
2008 - Present Emily Andreae, Function of Thrombin in the Cornea, Medical College of Wisconsin |
MS Students Advised |
1996 - 1998 Mark Nickolas, Gene Structure of Corneal Maspin, Medical College of Wisconsin |
Postdoctoral Students |
1989 - 1990 Susan Kirshner, DVM, Medical College of Wisconsin |
1991 - 1996 Xiaoye Zhou, MD, Medical College of Wisconsin |
2000 - 2002 Monica Olague-Marchan, PhD, Medical College of Wisconsin |
Clinical/Research Fellows |
2001 Blythe Janowiak, primary mentor, Medical College of Wisconsin |
2003 Kasia Broniowska, Medical College of Wisconsin |
2004 Brajesh Kumar, primary mentor, Medical College of Wisconsin |
2004 Robert Walchak, Medical College of Wisconsin |
2005 Malathi Narayan, Medical College of Wisconsin |
2005 Wei Gauk, Medical College of Wisconsin |
2006 Kate Merath, primary mentor, Medical College of Wisconsin |
2007 Susan Salti, primary mentor, Medical College of Wisconsin |
2007 Sreemanti Basu, Medical College of Wisconsin |
2007 Kathrine Schmalzer, Medical College of Wisconsin |
2007 Jackie Porath, Medical College of Wisconsin |
2007 Luis Paniagua, primary mentor, Medical College of Wisconsin |
2007 Rebecca Bark, primary mentor, Medical College of Wisconsin |
2008 Ben Tourdot, Medical College of Wisconsin |
2008 Kim Agle, Medical College of Wisconsin |
2008 Emily Andreae, primary mentor, Medical College of Wisconsin |
2010 Nathan Schuld, Medical College of Wisconsin |
EXTRAMURAL STUDENTS, FACULTY, RESIDENTS, AND CLINICAL/RESEARCH FELLOWS MENTORED: |
High School Students |
1991 - 1992 Brent Gilmore, Marquette High School |
2000 Anna Marie Vu, Glendale High School |
2007 - 2008, Students Modeling a Research Topic (Smart) Team (MSOE), Brown Dear High School |
2008 - 2009, Students Modeling a Research Topic (Smart) Team (MSOE), Lincoln High School, Manitowoc |
2008 Antonica Amour, Pulaski High School |
2009 - 2010, Students Modeling a Research Topic (Smart) Team (MSOE), Messmer High School, Milwaukee |
2010 Rupinder Grewal, Cudahy High School |
2010 - 2011, Students Modeling a Research Topic (Smart) Team (MSOE), St. Joan Antidia |
Undergraduate Students |
1984 Kevin Foley, Beloit College |
1987 Stephen Everse, Beloit College |
1991 Lisa Mahnke, Ripon College |
1994 Andrew Weber, University of Wisconsin, Stevens Point |
1999 Priscilla Moore, University of Wisconsin-Green Bay |
2000 Chris Miller, University of Dallas |
2001 Hieu Luu, Marquette University |
2003 - 2004 Tyna MacGill, Carroll College |
Graduate Students |
PhD Committees |
1985 - 1987 Vincent Braganza, S.J., Department of Biochemistry, Tryptase, A Novel Trypsin Like Rat Skin Enzyme: Isolation and Purification, Loyola University Medical School |
1998 - 1999 Kevin Wee, Department of Biology, Proteinases of Nematode Bacterial Parasites, Temple University |
COMMUNITY SERVICE ACTIVITIES: |
1983 - 1985 S.E. Wisconsin Science and Engineering Fair-Judge |
1985 - 1988 First Presbyterian Church, Oostburg, WI-Sunday School Teacher |
1985 - 1988 New Berlin Public Schools-High Interest Day |
1989 - 1991 Our Lords’ Methodist Church, New Berlin, WI-Sunday School Teacher |
1989 - 1990 Waukesha County Career Exposition |
1992 March of Dimes-Neighborhood Solicitor |
NATIONAL/INTERNATIONAL MEETING MODERATOR/ORGANIZER: |
1993 Organizer, Proteinase Interest Group Meeting, Control of proteolysis in the eye, Association for Research in Vision and Ophthalmology, Sarasota, FL |
1994 Organizer, Proteinase Interest Group Meeting, Functional effects of proteolysis on proteins in the eye, Association For Research In Vision And Ophthalmology, Sarasota, FL |
1994 Moderator, "Metalloproteinases" Poster Session, Association for Research in Vision and Ophthalmology, Sarasota, FL |
1997 Moderator, "Extracellular Matrix", Platform Session, Association for Research in Vision and Ophthalmology, Ft. Lauderdale, FL |
1998 Moderator, Pseudomonas Infections, Platform Session, Association For Research In Vision And Ophthalmology, Ft. Lauderdale, FL |
2005 Organizer and Moderator, Corneal Nerves, Minisymposium, Association for Research in Vision and Ophthalmology, Ft. Lauderdale, FL |
11/2006 Moderator, IRBP: A Journey Toward Understanding the Retina, A Tribute to Dr. Barbara Wiggert, NIH |
2006 Co-organizer and Moderator, Proteases: Substrate Cleavage and Beyond, ICER, Buenos Aires, Argentina |
2006 Organizer and Moderator, Scar Wars, Sunday Symposium, Association for Research in Vision and Ophthalmology, Ft. Lauderdale, FL |
BIBLIOGRAPHY |
Refereed Journal Publications/Original Papers |
1. TWINING SS. Newman DS Radiotracer studies of metal metal ion exchange inhibition using organic inhibitors. J. Electrochem. Soc. ll9:l292 l297, l972 |
2. TWINING SS. Brecher AS. Isolation and identification of α1-antitrypsin as a component of normal and malignant human breast and other tissues. Proc. Soc. Exper. Biol. Med. 150:98-103, 1975 |
3. TWINING SS. Brecher AS. Identification of α1-acid glycoprotein, α2-macroglobulin and antithrombin III as components of normal and malignant human tissues. Clin.Chim. Acta. 75:143-148, 1977 |
4. TWINING SS. Brecher AS. Characterization of the interaction between chymotrypsin and heparin. Can. J. Biochem. 55:134-13109, 1977 |
5. TWINING SS. Brecher AS. Large scale separation of protease inhibitors from malignant human breast tissue. Mol. Cell. Biochem. 18:101-7, 1977 |
6. TWINING SS. LaPoint D. Brecher A.S. Interaction of mucopolysaccharides with chymotrypsin. Polymers 18:483-488, 1977 |
7. Newman DS. Frank C. Matlack RW. TWINING SS Kristnan V. The ionic Hall effect in the solid electrolyte C5H6N.AgI6, Electrochim. Acta 22: 811-814, 1977 |
8. TWINING SS. Atassi MZ. Antibody-combining sites can be mimicked synthetically. Surface-simulation synthesis of the immunoglobulin new combining site to the gamma-hydroxyl derivative of vitamin K1. J. Biol. Chem. 253:5259-5262, 1978 |
9. Okuda K. Christadoss PR. TWINING SS Atassi MZ. David CS. Genetic control of immune response to sperm whale myoglobin in mice. I. T lymphocyte proliferative response under H-2-linked Ir gene control. J. Immunol. 121:866-868, 1978 |
10. Okuda K. TWINING SS. David CS. Atassi MZ. Genetic control of immune response to sperm whale myoglobin in mice. II. T lymphocyte proliferative response to the synthetic antigenic sites. J. Immunol. 123:182-188, 1979 |
11. TWINING SS. Atassi MZ. Use of immunoadsorbents for the study of antibody binding to sperm whale myoglobin and its synthetic antigenic sites. J. Immunol. Meth. 30:139-51, 1979 |
12. TWINING SS. Lehmann H. Atassi MZ. The antibody response to myoglobin is independent of the immunized species. Analysis in terms of replacements in the antigenic sites and in environmental residues of the cross-reactions of fifteen myoglobins with sperm-whale myoglobin antisera raised in different species. Biochem. J. 191:681-697, 1980 |
13. TWINING SS. Sealy RC. Glick DM. Preparation and activation of a spin-labeled pepsinogen. Biochemistry. 20:1267-72, 1981 |
14. TWINING SS. David CS. Atassi MZ. Genetic control of the immune response to myoglobin. IV. Mouse antibodies in outbred and congenic strains against sperm-whale myoglobin recognize the same antigenic sites that are recognized by antibodies raised in other species. Mol. Immunol. 18:447-450, 1981 |
15. TWINING SS. Lehmann H. Atassi MZ. Time-dependent study of the antibody response to sperm-whale myoglobin: recognition of the antigenic sites is unaltered over an extended period of immunization. Mol. Immunol. 18:473-479, 1981 |
16. Atassi MZ. TWINING SS. Lehmann H. David CS. Genetic control of the immune response to myoglobin. V. Analysis of the cross-reactivity of 12 myoglobins with sperm-whale myoglobin antisera of inbred mouse strains in terms of substitutions in the antigenic sites and in the environmental residues of the sites. Immunol. Commun. 10:359-365, 1981 |
17. Atassi MZ. Yokota S. TWINING SS. Lehmann H. David CS. Genetic control of the immune response to myoglobin. VI. Inter-site influences in T-lymphocyte proliferative response from analysis of cross-reactions of ten myoglobins in terms of substitutions in the antigenic sites and in environmental residues of the sites. Mol. Immunol. 18:945-948, 1981 |
18. TWINING SS. Alexander PA. Huibregtse K. Glick DM. A pepsinogen from rainbow trout. Comp. Biochem. Physiol. - B: Comp. Biochem. 75:109-112, 1983 |
19. TWINING SS. Huibregtse K. Glick DM. A pepsinogen from dog stomach. Comp. Biochem. Physiol. - B: Comp. Biochem. 75:103-107, 1983 |
20. TWINING S. Potts D. Burke J. Acid proteases of vitreal macrophages. Curr. Eye Res. 3:1055-1062, 1984 |
21. TWINING SS. Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes. Anal .Biochem. 143:30-34, 1984 |
22. TWINING SS. Hatchell DL. Hyndiuk RA. Nassif KF. Acid proteases and histologic correlations in experimental ulceration in vitamin A deficient rabbit corneas. Invest. Ophthalmol. Vis. Sci. 26:31-44, 1985 |
23. TWINING SS. Lohr KM. Moulder JE. The immune system in experimental Pseudomonas keratitis. Model and early effects. Invest. Ophthalmol. Vis. Sci. 27:507-515, 1986 |
24. Ripple RE. Lohr KM. TWINING SS. Hyndiuk RA. Caya JG. Role of leukocytes in ocular inflammation of tyrosinemia II. Invest. Ophthalmol. Vis. Sci. 27:926-931, 1986 |
25. TWINING SS. Davis SD. Hyndiuk RA. Relationship between proteases and descemetocele formation in experimental Pseudomonas keratitis. Curr. Eye Res.5:503-510, 1986 |
26. Burke JM. TWINING SS. Vitreous macrophage elicitation: generation of stimulants for pigment epithelium in vitro. Invest. Ophthalmol. Vis. Sci. 28:1100-1107, 1987 |
27. Burke JM. TWINING SS. Regional comparisons of cathepsin D activity in bovine retinal pigment epithelium. Invest. Ophthalmol. Vis. Sci. 29:1789-1793, 1988 |
28. TWINING SS. †Everse SJ. Wilson PM. Yue BY. Chan SK. Localization and quantification of alpha-1-proteinase inhibitor in the human cornea. Curr. Eye Res. 8:389-395, 1989 |
29. TWINING SS. Wilson PM. Hatchell DL. Characterization of corneal proteoglycans under vitamin A deficiency. Biochim. Biophys. Acta. 992:181-190, 1989 |
30. Sawaguchi S. TWINING SS. Yue BY. Wilson PM. Sugar J. Chan SK. α-1 Proteinase inhibitor levels in keratoconus. Exp. Eye Res. 50:549-554, 1990 |
31. Kangas TA. Edelhauser HF. TWINING SS. O'Brien WJ. Loss of stromal glycosaminoglycans during corneal edema. Invest. Ophthalmol. Vis. Sci. 31:1994-2002, 1990 |
32. TWINING SS. Kirschner SE. Mahnke LA. Frank DW. Effect of Pseudomonas aeruginosa elastase, alkaline protease, and exotoxin A on corneal proteinases and proteins. Invest. Ophthalmol. Vis. Sci. 34:2699-712, 1993 |
33. Ando H. TWINING SS. Yue BY. Zhou X. Fini ME. Kaiya T. Higginbotham EJ. Sugar J. MMPs and proteinase inhibitors in the human aqueous humor. Invest. Ophthalmol. Vis. Sci. 34:3541-3548, 1993 |
34. Sawaguchi S. Yue BY. Kawa JE. Chang IL. TWINING SS. Meberg B. Lysosomal enzyme and inhibitor levels in the human trabecular meshwork Invest. Ophthalmol. Vis. Sci. 35:251-261, 1994 |
35. Brecher AS. TWINING SS. Brecknock BB. Friessen JA. Long J.E. Friedberg F. Organic solvents and Ca2+ influence the interaction of alkylating agents with chymotrypsin. Mol. Biol. (Life Sci. Adv.) 13:157-163, 1994 |
36. TWINING SS. Fukuchi T. Yue BY. Wilson PM. Boskovic G. Corneal synthesis of alpha 1-proteinase inhibitor (alpha 1-antitrypsin). Invest. Ophthalmol. Vis. Sci. 35:458-462, 1994 |
37. TWINING SS. Fukuchi T. Yue BY. Wilson PM. Zhou X.†Loushin G. α 2-Macroglobulin is present in and synthesized by the cornea. Invest. Ophthalmol. Vis. Sci. 35:3226-3233, 1994 |
38. TWINING SS. Fukuchi T. Yue BY. Wilson PM. Zhou X. α1-Antichymotrypsin is present in and synthesized by the cornea. Curr. Eye Res. 13:433-439, 1994 |
39. Sawaguchi S. TWINING SS. Yue BY. Chang SH. Zhou X. Loushin G. Sugar J. Feder RS. α 2-Macroglobulin levels in normal human and keratoconus corneas. Invest. Ophthalmol. Vis. Sci. 35:4008-4014, 1994 |
40. TWINING SS. Zhou X. Schulte DP. Wilson PM. Fish B. Moulder J. Effect of vitamin A deficiency on the early response to experimental Pseudomonas keratitis. Invest. Ophthalmol. Vis. Sci. 37:511-522, 1996 |
41. TWINING SS. Schulte DP. Wilson PM. Fish BL. Moulder JE. Retinol is sequestered in the bone marrow of vitamin A-deficient rats. J. Nutr. 126:1618-1626, 1996 |
42. Brecher AS. Hinko A. TWINING SS. A proposed sequence of reactions leading to collagen biosynthesis, fibrosis, and hypertension in alcoholics. J. Invest. Med. 44:36-41, 1996 |
43. TWINING SS. Schulte DP. Wilson PM. Zhou X. Fish BL. Moulder JE. Neutrophil cathepsin G is specifically decreased under vitamin A deficiency. Biochim. Biophys. Acta-Mol. Basis Dis. 1317:112-118, 1996 |
44. Zhou L. Yue BY. TWINING SS. Sugar J. Feder RS. Expression of wound healing and stress-related proteins in keratoconus corneas. Curr. Eye Res.15:1124-1131, 1996 |
45. Whitelock RB. Fukuchi T. Zhou L. TWINING SS. Sugar J. Feder RS. Yue BY. Cathepsin G, acid phosphatase, and α1-proteinase inhibitor messenger RNA levels in keratoconus corneas. Invest. Ophthalmol. Vis. Sci. 38:529-534, 1997 |
46. TWINING SS. Schulte DP. Wilson PM. Fish BL. Moulder JE. Vitamin A deficiency alters rat neutrophil function. J. Nutr. 127:558-565, 1997 |
47. TWINING SS. Schulte DP. Zhou X. Wilson PM. Fish BL. Moulder JE. Changes in rat corneal matrix metalloproteinases and serine proteinases under vitamin A deficiency. Curr. Eye Res.16:158-165, 1997 |
48. Boskovic G. TWINING SS. Retinol and retinaldehyde specifically increase α1-proteinase inhibitor in the human cornea. Biochem. J. 322:751-756, 1997 |
49. Li Y. Zhou L. TWINING SS. Sugar J. Yue BY. Involvement of Sp1 elements in the promoter activity of the α1-proteinase inhibitor gene. J. Biol. Chem. 273:9959-9965, 1998 |
50. Zhou L. Sawaguchi S. TWINING SS. Sugar J. Feder RS. Yue BY. Expression of degradative enzymes and protease inhibitors in corneas with keratoconus. Invest. Ophthalmol. Vis. Sci. 39:1117-1124, 1998 |
51. Sawa T. Ohara M. Kurahashi K. TWINING SS. Frank DW. Doroques DB. Long T. Gropper MA. Wiener-Kronish JP. In vitro cellular toxicity predicts Pseudomonas aeruginosa virulence in lung infections. Infect. Immun. 66:3242-3249, 1998 |
52. Boskovic G. TWINING SS. Local control of α1-proteinase inhibitor levels: regulation of α1-proteinase inhibitor in the human cornea by growth factors and cytokines. Biochim. Biophys. Acta. -Mol. Cell Res. 1403:37-46, 1998 |
53. TWINING SS. Wilson PM. Ngamkitidechakul C. Extrahepatic synthesis of plasminogen in the human cornea is up-regulated by interleukins-1α and -1β. Biochem. J. 339:705-712, 1999 |
54. Reiter SA. McGill CR. Lawrence SL. TWINING SS. Blood glucose laboratory for first-year medical students: an interdisciplinary model for nutrition-focused diabetes management. J Amer. Diet. Assoc. 100:570-572, 2000 |
55. Liu Z. Shapiro SD. Zhou X. TWINING SS. Senior RM. Giudice GJ. Fairley JA. Diaz LA. A critical role for neutrophil elastase in experimental bullous pemphigoid. J. Clin. Invest. 105:113-123, 2000 |
56. Olague-Marchan M. TWINING SS. Hacker MK. McGrath JA. Diaz LA. Giudice GJ. A disease-associated glycine substitution in BP180 (type XVII collagen) leads to a local destabilization of the major collagen triple helix. Matrix Biol. 19:223-233, 2000 |
57. Liu Z. Zhou X. Shapiro SD. Shipley JM. TWINING SS. Diaz LA. Senior RM. Werb Z. The serpin α1-proteinase inhibitor is a critical substrate for gelatinase B/MMP-9 in vivo. Cell. 102:647-655, 2000 |
58. TWINING SS, Goryshin IY, Bhasin A, Reznikoff WS. Functional characterization of arginine 30, lysine 40 and arginine 62 in Tn5 transposase. J. Biol. Chem. 276, 23135-23143, 2001 |
59. Ngamkitidechakul C. Burke JM. O'Brien WJ. TWINING SS. Maspin: synthesis by human cornea and regulation of in vitro stromal cell adhesion to extracellular matrix. Invest. Ophthalmol. Vis. Sci. 42, 3135-3141, 2001 |
60. Ngamkitidechakul, C, TWINING SS. Buffered non-fermenter system for lab-scale production of secreted recombinant His-tagged proteins in Saccharomyces cerevisiae. Biotechniques, 33, 1296-1300, 2002 |
61. Ngamkitidechakul C, Warejcka DJ, Burke JM, O'Brien WJ, TWINING SS. Sufficiency of the reactive site loop of maspin for Induction of cell-matrix adhesion and inhibition of cell invasion: Conversion of ovalbumin to a maspin-like molecule. J Biol Chem. 278, 31796-31806, 2003 |
62. Cowell BA, TWINING SS. Hobden JA. Kwong MSF. Fleiszig SMJ. Mutation of LasA and LasB reduces Pseudomonas aeruginosa invasion of epithelial cells. Microbiology 2291-2299, 2003 |
63. Rogers CS, Chan L-M., Sims,YS, Byrd, KD, Hinton, DL, TWINING, SS. The effects of sub-solar levels of UV-A and UV-B on rabbit corneal and lens epithelial cells, Exp. Eye Res.1007-1014, 2004 |
64. Ayala A. Warjecka DJ. Vaughan K. Yue BYJT. TWINING, SS. The Fibrinolysis Inhibitor a2-Antiplasmin in the Human Cornea, Curr. Eye Res. 1097-1103, 2005 |
65. Warejcka DJ. TWINING, SS. Specific conformational changes of plasminogen induced by chloride ions, 6-aminohexanoic acid and benzamidine but not the overall openness of plasminogen, Biochem. J. 392, 703-712, 2005 |
66. Warejcka DJ. Vaughan KA. Bernstein AM. TWINING, SS. Differential conversion of plasminogen to angiostatin by human corneal cell populations, Mol. Vis. 47, 859-868, 2005 |
67. Marquart, M.E. Caballero, A.R.. Traidej, M, Thibodeaus, BA. TWINING, SS. O'Callaghan, RJ. Identification of a small secreted protease from Pseudomonas aeruginosa. Invest. Ophthalmol. Vis. Sci., 46, 3761-3768, 2005 |
68. Ayala A. Warejcka DJ., Olague-Marchan, M. TWINING, SS. Corneal activation of prothrombin to thrombin independent of vascular injury, Invest. Ophthalmol. Vis. Sci., 48, 134-143, 2007 |
69. Bernstein, AM,.TWINING, SS. Warejcka, DJ Tall, E. Masur, SK, Regulation of the Urokinase Pathway by Corneal Fibroblasts and Myofibroblasts, Mol. Biol. Cell, 18, 2716-2727, 2007 |
70. Horswill MA. Narayan M. Warejcka DJ. Cirillo LA TWINING SS, Epigenetic silencing of maspin expression occurs early in the conversion of keratocytes to fibroblasts. Exp Eye Res. 86, 586-600, 2008 (Cancer Related) |
71. Bohnsack RN. Patel M. Olson LJ. TWINING SS. Dahms NM. Residues Essential for Plasminogen Binding by the Cation-Independent Mannose 6-Phosphate Receptor. Biochemistry. 49, 635-644, 2010 |
72. White MJ. He H. Penoske RM. TWINING SS. and Zahrt TC. PepD participates in the Mycobacterial stress response mediated through MprAB and SigE. J.Bacteriol. 192,1498-510, 2010 |
73. Narayan M. Mirza SP. TWINING, SS. Identification of phosphorylation sites on extracellular corneal epithelial cell maspin. Proteomics. 11,1382-90, 2011. (Cancer Related) |
74. Endsley MP. Hu Y. Deng Y. He X. Warejcka DJ. TWINING SS. Gonias SL. Zhang M. Maspin: the molecular bridge between the plasminogen activator system and β1 integrin that facilitates cell adhesion. J Biol Chem. 2011 online (Cancer Related) |
75. Warejcka, DJ, Narayan, M, TWINING, SS, Maspin increases extracellular plasminogen activator activity associated with corneal fibroblasts and myofibroblasts, Exp. Eye Res., 2011, In press (Cancer Related) |
Books, Chapters, and Reviews |
1. TWINING SS. Wilson PM. Hyndiuk, RA. Proteases and proteoglycans in vitamin A deficiency the cornea: Transactions of the world congress of the cornea III, Raven Press, NY, NY, p. l2l l24, l988 |
2. Nauss KM. Ross AC. TWINING SS. Immunotrophic methodology. Meth. Enzymol. 190:259-73, 1990 |
3. TWINING SS. Wilson PM. Metal sensitive protease(s) associated with corneal proteoglycans. Matrix Suppl. 1:91-2, 1992 |
4. TWINING SS. Regulation of proteolytic activity in tissues. Crit. Rev. Biochem. Mol. Biol. 29:315-83, 1994 |
5. Reznikoff WS. Bhasin A. Davies DR. Goryshin IY. Mahnke LA. Naumann T. Rayment I. Steiniger-White M. TWINING SS. Tn5: A molecular window on transposition. Biochem.Biophys. Res. Commun. 266:729-34, 1999 |
6. Narayan, M. TWINING S. Focus on Molecules: maspin, Exp. Eye. Res. 90, 2-3, 2010. |
Abstracts |
1. l. SHINEW S. Newman DS. Radiotracer studies of metal metal ion exchange inhibition using organic inhibitors, Abstracts of the National Meeting of the Electrochemical Society, Cleveland, Ohio, 1971 |
2. Newman DS. Frank C. Matlack RW. TWINING SS. The ionic Hall effect in the solid electrolyte C5H6 NI.8AgI, Abstracts of the National Meeting of the Electrochemical Society, Chicago, Illinois, l973 |
3. TWINING SS. Brecher AS. Affinity chromatography of protease inhibitors in malignant human breast tissue, Abstracts of the American Chemical Society Meeting, Atlantic City, New Jersey, l974 |
4. TWINING SS. Brecher AS. Polyacrylamide disc gel electrophoresis evidence of a complex between chymotrypsin and heparin, Fed. Proc. 34, 2362, l975 |
5. TWINING SS. LaPointe D. Brecher AS. Interaction of mucopolysaccharides with chymotrypsin, Fed. Proc. 35, l445, l976 |
6. TWINING SS. Brecher AS. Large-scale separation of protease inhibitors from malignant human breast tissue, First Cleveland Symposium on Macromolecules, Cleveland, Ohio. l976 |
7. Okuda K. TWINING SS. Atassi MZ. David C. Genetic control of t lymphocyte proliferative response to sperm whale myoglobin and its synthetic antigenic sites in mice, Abstracts of the Fourth Ir Gene Workshop, Annapolis, Maryland, l978 |
8. TWINING SS. Sealy RC. Cataldi M. Glick DM. Electron spin resonance studies on the activation of pepsinogen, Fed. Proc. 39, l685, l980 |
9. TWINING SS. Increase in cathepsin D activity by human breast tissue components, Gordon Research Conference on Proteolytic Enzymes and their Inhibitors, Plymouth, New Hampshire, l980 |
10. l0. Glick DM. TWINING SS. Sealy RC. Some kinetic studies on pepsinogen activation, Gordon Research Conference on Proteolytic Enzymes and their Inhibitors, Plymouth, New Hampshire, l980 |
11. ll. TWINING SS. Huibregtse K. Glick DM. Purification of acid proteases from dog stomach, Fed. Proc. 40, l7l9, l98l |
12. l2. TWINING SS. Alexander P. Glick DM. Characteristics of a new intermediate in pepsinogen activation, Fed. Proc. 4l, 762, l982 |
13. l3. TWINING SS. Hatchell DL. Proteases and protease inhibitors in normal and ulcerating vitamin a deficient rabbit corneas, Gordon Research Conference of Proteolytic Enzymes and their Inhibitors, Plymouth, New Hampshire, l982 |
14. l4. TWINING SS. Hatchell DL. Acid proteases in keratomalacia, Invest. Ophthalmol. Suppl. 24, 44, l983 |
15. l5. TWINING SS. Fluorescein isothiocyanate casein assay for proteolytic enzymes, Fed. Proc. 42, l95l, l983 |
16. l6. TWINING SS. Potts D. Burke JM. Acid proteases of vitreal macrophages, Invest. Ophthalmol. Vis. Sci. 25S, 207, l984 |
17. l7. Huntemann S. Essma JB TWINING SS. Effect of vitamin A deficiency on corneal aminopeptidases, Fed. Proc. 43, 2067, l984 |
18. l8. TWINING SS. Hatchell DL. Vitamin A and the Retinoids, Effects of vitamin A deficiency on corneal proteases, FASEB Conference on Micronutrients, Saxton River, Vermont, l984 |
19. l9. TWINING SS. Lohr KM. and Moulder JE. The immune system in Pseudomonas keratitis: model and early effects, Invest. Ophthalmol. Vis. Sci. 26S, 27l, l985 |
20. TWINING SS. Wilson PM., Isolation of a metalloprotease from normal rabbit cornea, l3th International Congress of Biochemistry, Amsterdam, l985 |
21. 2l. Burke JM. TWINING SS., Generation of growth and chemotaxis stimulants by elicitation of a macrophage invasion into rabbit vitreous, Invest. Ophthalmol. Vis. Sci. 27S, l88, l986 |
22. TWINING SS.Davis SD. Hyndiuk RA. Proteases and descemetoceles in experimental Pseudomonas keratitis, Invest. Ophthalmol. Vis. Sci. 27S, 209, l986 |
23. TWINING SS. Wilson PM., Corneal proteoglycan changes under vitamin a deficiency, Fed. Proc. 45, 2ll9,l986 |
24. TWINING SS. Wilson PM., Proteolytic susceptibility of corneal proteoglycans from normal and vitamin a deficient rabbits, Fed. Proc. 46, 2202, l987 |
25. TWINING SS. Wilson PM., Characterization of corneal protease inhibitors; localization of l protease inhibitor in the cornea, Strangeways Research Laboratory 75th Anniversary Symposium on The Control of Tissue Damage, l987 |
26. TWINING SS. Everse SJ. and Chan S K. Presence of l-protease inhibitor ( l antitrypsin) in the human cornea, Invest. Ophthalmol. Vis. Sci. 29S, l988 |
27. Burke JM. TWINING SS., Cathepsin D activity in bovine RPE: Comparison of cells from the posterior and equatorial regions, Invest. Ophthalmol. Vis. Sci. 29S, 90, l988 |
28. Kangas TA. Edelhauser HF. TWINING SS. O'Brien WJ. Loss of stromal proteoglycans during corneal edema, Invest. Ophthalmol. Vis. Sci. 29S, 2l5, l988 |
29. TWINING SS., Wilson PM. Yue B. Chan S-K. l Proteinase inhibitor in the human cornea. Effect of keratoconus on the l protease, Gordon Conference on Proteolytic Enzymes and Their Inhibitors, l988 |
30. TWINING SS., Kawczyinski RJ. Wilson PM. Moulder J. Fish B. McFadden P. Koethe S. Effect of Vitamin A deficiency on Pseudomonas aeruginosa infections and polymorphonuclear leukocyte function, FASEB Conference on the Retinoids, l988 |
31. 3l. TWINING SS., Kawczyinski RJ. McFadden P. Simultaneous quantification of phagocytosis and killing by leukocytes using fluorescence flow cytometry, J. Cell Biol. 107, 574a, l988 |
32. TWINING SS. Wilson PM., Isolation of corneal stromal proteoglycan-protease complexes, Invest. Ophthalmol. Vis. Sci. 30S, 205, 1989 |
33. Sawaguchi S. TWINING SS. Yue B. Wilson, PM. Sugar J. Alpha-1-proteinase inhibitor levels in keratoconus, Invest. Ophthalmol. Vis. Sci. 30S, 474, 1989 |
34. TWINING SS. Wilson PM., Metal-sensitive protease associated with corneal proteoglycans, matrix metalloproteinase Conference, Destin, Fla., 1989 |
35. TWINING SS. Wilson PM., Influence of polymorphonuclear leukocyte secreted products on degradation of corneal proteins and proteoglycans, Invest. Ophthalmol. Vis. Sci. 31S, 1990 |
36. Kirschner SE. TWINING SS. The effect of Pseudomonas aeruginosa exoproducts on corneal proteases, Invest. Ophthalmol. Vis. Sci. 31S, 1990 |
37. TWINING SS., Wilson PM. Zhou X. Synthesis of proteinase inhibitors in the cornea: immunoprecipitation of metabolically labeled proteins with monoclonal antibodies to -1-proteinase inhibitor and -2-macroglobulin, FASEB J. 5, A828, 1991 |
38. Friedberg F. TWINING SS. Brecknock BB. Long JE.Friesen J. Brecher AS. Chymotrypsin reactivity with alkylating agents is altered by solvent and ion effects, FASEB J. 5, A450, 1991 |
39. TWINING SS., Wilson PM. Zhou X. Identification of proteinase inhibitors in the cornea: -1-antichymotrypsin, -2-antiplasmin and -2-macroglobulin, Invest. Ophthalmol. Vis. Sci. 32S, 1071, 1999 |
40. TWINING SS., Wilson PM. ‡Zhou X. Presence and synthesis of serine proteinase inhibitors in the cornea, International Congress of Biochemistry, 1991 |
41. 41 Yue B. Loushin G. Sawaguchi S. TWINING S. Sugar J. 2-Macroglobulin levels in keratoconus, Invest. Ophthalmol. Vis. Sci. 33, 976. 1992 |
42. TWINING SS. Schulte DP. Effect of Vitamin A deficiency on neutrophil function in the cornea and in vitro, Invest. Ophthalmol. Vis. Sci. 34, 775, 1992 |
43. TWINING SS., Isolation and characterization of a new proteinase from Pseudomonas aeruginosa. Invest. Ophthalmol. Vis. Sci. 35, 847, 1993 |
44. Boskovic GD. TWINING SS., Effect of growth factors and cytokines on the expression of 1-proteinase inhibitor in the human cornea, FASEB J. 1993 |
45. TWINING SS. Boskovic GD. Stimulation of 1-proteinase inhibitor synthesis by cytokines and retinol, Invest. Ophthalmol. Vis. Sci. 35, 1994 |
46. TWINING SS. Zhou X. Wilson PM. Schulte DP. Effect of vitamin A deficiency on corneal proteinases, Invest. Ophthalmol. Vis. Sci. 36, S700, 1995 |
47. TWINING SS. Boskovic GD., Unique regulation of the synthesis of 1-proteinase inhibitor in the cornea, presented at the International Symposium on the Chemistry and Biology of SERPINS, Chapel Hill, NC, April 1996 |
48. TWINING SS. Schulte DP. Wilson PM. Retinol is sequestered in the bone marrow of Vitamin A deficient animals, Invest. Ophthalmol. Vis. Sci. 37, S853, 1996 |
49. TWINING SS. Corneal ulceration: disruption of the proteinase-inhibitor balance, Exp. Eye Res. 63, S17, 1996 |
50. Liu Z, TWINING SS Giudice GJ, Zhou X. Fairley JA. Diaz LA. Elastase plays a direct role in blister formation in experimental bullous pemphigoid, FASEB J. 10, A1407, 1996 |
51. TWINING SS. Wilson PM., Isolation and Characterization of human corneal serine proteinases, Invest. Ophthalmol. Vis Sci. 38, S3184, 1997 |
52. Yue B. Zhou L. Sawaguchi S, TWINING SS. Sugar J. Expression of degradative enzymes and protease inhibitors in keratoconus corneas, Invest. Ophthalmol. Vis Sci. 38, S4453, 1997 |
53. Li Y. Whitlock RB, TWINING SS, Sugar J. Yue B. Identification of regulatory elements in 1-proteinase inhibitor gene in releation to keratoconus, Invest. Ophthalmol. Vis Sci. 38, S5088, 1997 |
54. Liu Z. TWINING SS. Giudice GJ. Zhou X. Fairley JA. Troy JL. Diaz LA. Neutrophil elastase is essential for subepidermal blistering in experimental bullous pemphigoid, J. Invest. Derm. 108, 556A, 1997 |
55. TWINING SS. Ngamkitdechakul C. Nicholas M. Human corneal epithelial stromal and endothelial cells contain and synthesize the class II tumor suppressor maspin, Invest. Ophthalmol. Vis. Sci., 40, S393, 1999 |
56. Ngamkitdechakul C. TWINING SS., Non-epithelial synthesis of the tumor suppressor maspin in the human corneal, FASEB J., 13, A1538, 1999 |
57. TWINING SS., Sternard H. Angiostatin production by human corneal stromal cells, Invest. Ophthalmol. Vis. Sci. 41, S118, 2000 |
58. Sternard H, TWINING SS. Conversion of plasminogen to angiostatin-like molecules by corneal stromal cells, American Association for Cancer Research Meeting on Angiogenesis and Cancer, Traverse City, MI, 2000, |
59. TWINING SS. Ngamkitdechakul C. Biological effects of the tumor suppressor maspin on corneal stromal cell movement and cell-extracellular matrix adhesion, Invest. Ophthalmol. Vis. Sci. 42, S279, 2001 |
60. Ngamkitdechakul C. Burke JM. O'Brien, WJ. TWINING SS. Identification of functional domain(s) of maspin for enhancement of corneal stromal cell adhesion to Type I collagen, Mol.Biol. Cell 12, 190a, 2001 |
61. TWINING SS. Olague-Marchan M. Ayala A. Evidence for an extravascular corneal coagulation system, Invest. Ophthalmol. Vis. Sci. 2002 43: E-Abstract 3202 |
62. TWINING SS. Ngamkitdechakul C. The RSL of maspin is required for induction of cell-matrix adhesion: conversion of ovalbumin to a maspin-like molecule. 3rd International Symposium on Serpin Biology Structure and Function, Chicago, IL, 2002 |
63. Warejcka DJ. TWINING SS. Benzamidine binding to plasminogen changes availability of cleavage sites for human plasminogen. Experimental Biology 2003, San Diego, CA, 2003 |
64. TWINING SS. Ngamkitidechakul C. O'Brien WJ. Burke JM. Sufficiency of the reactive site loop of maspin for induction of keratocyte-matrix adhesion: Conversion of ovalbumin to a maspin-like molecule. Invest. Ophthalmol. Vis. Sci. 2003 44: E-Abstract 871 |
65. TWINING SS. Vaughan, KA. Warejcka,DJ, Effects of maspin on corneal stromal cell gene expression: Increased protein levels of SPARC and PAI-1, Invest. Ophthalmol. Vis. Sci. 2004 45: E-Abstract 4551 |
66. Bernstein, AM. TWINING SS. Vaughan, KA. Masur SK, Urokinase pathway regulation in corneal fibroblasts and myofibroblasts, Invest. Ophthalmol. Vis. Sci. 2005 46: E-Abstract 2304 |
67. TWINING SS. Warejcka, DJ. Bernstein AM. Corneal epithelial cells and stromal fibroblasts but not stromal myofibroblasts efficiency convert plasminogen to angiostatin, Invest. Ophthalmol. Vis. Sci. 2005 46: E-Abstract 2187 |
68. TWINING SS, Narayan M, Warejcka DJ, Berg T, and Horswill MA, Maspin Mediated Alteration of The Actin Cytoskeleton In Human Corneal Stromal Fibroblasts, Invest. Ophthalmol. Vis. Sci. 2006 47: E-Abstract 2980 |
69. TWINING SS, Warejcka DJ, Ayala A, Angiostatin formation and thrombin generation for activation of protease activated receptors and fibrin formation, International Congress of Eye Research, Buenos Aires, October 2006 |
70. TWINING SS, Warejcka, DJ. Maspin Increases Activation of Plasminogen by Corneal Stromal Fibroblasts and Myofibroblasts, American Society of Cell Biology, Annual Meeting, Washington, DC, December 2007 |
71. TWINING, SS, Warejcka, D.J., The Effect of Thrombin on the Plasminogen-Plasmin System in the Cornea. Invest. Ophthalmol. Vis. Sci. 2008 49: E-Abstract 2940 |
72. Narayan, M., Horswil, M.A., Warejcka, D.J., TWINING, SS, Regulation of Maspin Expression by DNA and Histone Methylation During Corneal Wound Healing, Invest. Ophthalmol. Vis. Sci. 2008 49: E-Abstract 3371 |
73. Denson K, Brown D, Duthie E, Sabina R, TWINING S, Derse A, Liard J., Teaching Geriatrics Outside the Clinics: Integrating Geriatrics into Basic Science, American Geriaritics Society National Meeting, Chicago, IL May 2009 |
74. Narayan M, Mirza, SP, TWINING SS., Identification of Phosphorylation Sites on Maspin Secreted by Corneal Epithelial Cells using Tandem Mass Spectrometry, Invest. Ophthalmol. Vis. Sci. 2009 50: E-Abstract 4603 |
75. Andreae EA, TWINING SS, Thrombin Induces the Expression of Cyr61 in Human Corneal Cells, Invest. Ophthalmol. Vis. Sci. 2009 50: E-Abstract 5698 |
76. Marcdante KA, TWINING, S.S., A Team Based Learning Approach to Translational Research, Conference of the Association of American Medical Colleges Central Group on Educational Affairs, Rochester MN March 2009 |
77. Andreae, EA, TWINING, S.S. Thrombin Affects Cyr61 Synthesis and Processing through Two Distinct Mechanisms, Invest. Ophthalmol. Vis Sci. 2010 51 E-abstract 1976 |
78. Narayan M, Mirza SP, TWINING SS, Differential Phosphorylation of Intracellular and Secreted Corneal Epithelial Cell Maspin, Invest. Ophthalmol. Vis Sci. 2010 51 E-abstract 1972 |
79. Andreae EA, Warejcka DJ, TWINING SS, Analysis of Thrombin Activity and Cyr61 Regulation in a Rat Cornea Organ Culture System, Invest. Ophthalmol. Vis Sci. 2011 52 E-abstract 2025 |
