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Nicholas R. Silvaggi PhD

Nicholas R. Silvaggi PhD profile photo picture

Assistant Professor

Institution: University of Wisconsin - Milwaukee
Department: Chemistry and Biochemistry

Publications (35)

  • Molecular Basis for ADP-Ribose Binding to the Mac1 Domain of SARS-CoV-2 nsp3. (Frick DN, Virdi RS, Vuksanovic N, Dahal N, Silvaggi NR) Biochemistry 2020 Jul 21;59(28):2608-2615 PMID: 32578982 PMCID: PMC7341687 SCOPUS ID: 2-s2.0-85088493564 06/25/2020       77 Citations
  • Correction to "Streptomyces wadayamensis MppP is a PLP-Dependent Oxidase, Not an Oxygenase". (Han L, Vuksanovic N, Oehm SA, Fenske TG, Schwabacher AW, Silvaggi NR) Biochemistry 2019 Dec 10;58(49):5023 PMID: 31790219 SCOPUS ID: 2-s2.0-85076328917 12/04/2019    
  • Correction to "Mechanistic Studies of the Streptomyces bingchenggensis Aldolase-Dehydratase: Implications for Substrate and Reaction Specificity in the Acetoacetate Decarboxylase-like Superfamily". (Mydy LS, Hoppe RW, Hagemann TM, Schwabacher AW, Silvaggi NR) Biochemistry 2019 Dec 10;58(49):5022 PMID: 31790220 SCOPUS ID: 2-s2.0-85076298256 12/04/2019    
  • Mechanistic Studies of the Streptomyces bingchenggensis Aldolase-Dehydratase: Implications for Substrate and Reaction Specificity in the Acetoacetate Decarboxylase-like Superfamily. (Mydy LS, Hoppe RW, Hagemann TM, Schwabacher AW, Silvaggi NR) Biochemistry 2019 Oct 08;58(40):4136-4147 PMID: 31524380 SCOPUS ID: 2-s2.0-85072910764 09/17/2019       1 Citation
  • Transient-State Analysis of Human Isocitrate Dehydrogenase I: Accounting for the Interconversion of Active and Non-Active Conformational States. (Roman JV, Melkonian TR, Silvaggi NR, Moran GR) Biochemistry 2019 Dec 31;58(52):5366-5380 PMID: 31478653 SCOPUS ID: 2-s2.0-85077403304 09/04/2019       10 Citations
  • RitR is an archetype for a novel family of redox sensors in the streptococci that has evolved from two-component response regulators and is required for pneumococcal colonization. (Glanville DG, Han L, Maule AF, Woodacre A, Thanki D, Abdullah IT, Morrissey JA, Clarke TB, Yesilkaya H, Silvaggi NR, Ulijasz AT) PLoS Pathog 2018 May;14(5):e1007052 PMID: 29750817 PMCID: PMC5965902 SCOPUS ID: 2-s2.0-85047976604 05/12/2018       14 Citations
  • Streptomyces wadayamensis MppP is a PLP-Dependent Oxidase, Not an Oxygenase. (Han L, Vuksanovic N, Oehm SA, Fenske TG, Schwabacher AW, Silvaggi NR) Biochemistry 2018 Jun 12;57(23):3252-3264 PMID: 29473729 SCOPUS ID: 2-s2.0-85048443514 02/24/2018       15 Citations
  • Swit_4259, an acetoacetate decarboxylase-like enzyme from Sphingomonas wittichii RW1. (Mydy LS, Mashhadi Z, Knight TW, Fenske T, Hagemann T, Hoppe RW, Han L, Miller TR, Schwabacher AW, Silvaggi NR) Acta Crystallogr F Struct Biol Commun 2017 Dec 01;73(Pt 12):672-681 PMID: 29199988 PMCID: PMC5713672 SCOPUS ID: 2-s2.0-85037072903 12/05/2017       2 Citations
  • Small molecule metalloprotease inhibitor with in vitro, ex vivo and in vivo efficacy against botulinum neurotoxin serotype A. (Jacobson AR, Adler M, Silvaggi NR, Allen KN, Smith GM, Fredenburg RA, Stein RL, Park JB, Feng X, Shoemaker CB, Deshpande SS, Goodnough MC, Malizio CJ, Johnson EA, Pellett S, Tepp WH, Tzipori S) Toxicon 2017 Oct;137:36-47 PMID: 28698055 PMCID: PMC5694348 SCOPUS ID: 2-s2.0-85024473954 07/13/2017       10 Citations
  • Ligand binding phenomena that pertain to the metabolic function of renalase. (Beaupre BA, Roman JV, Hoag MR, Meneely KM, Silvaggi NR, Lamb AL, Moran GR) Arch Biochem Biophys 2016 Dec 15;612:46-56 PMID: 27769837 PMCID: PMC5522708 SCOPUS ID: 2-s2.0-84993978149 11/05/2016       12 Citations
  • Periplasmic Cytophaga hutchinsonii Endoglucanases Are Required for Use of Crystalline Cellulose as the Sole Source of Carbon and Energy. (Zhu Y, Han L, Hefferon KL, Silvaggi NR, Wilson DB, McBride MJ) Appl Environ Microbiol 2016 Aug 01;82(15):4835-4845 PMID: 27260354 PMCID: PMC4984284 SCOPUS ID: 2-s2.0-84979916872 06/05/2016       37 Citations
  • Synthesis and evaluation of vitamin D receptor-mediated activities of cholesterol and vitamin D metabolites. (Teske KA, Bogart JW, Sanchez LM, Yu OB, Preston JV, Cook JM, Silvaggi NR, Bikle DD, Arnold LA) Eur J Med Chem 2016 Feb 15;109:238-46 PMID: 26774929 PMCID: PMC4737989 SCOPUS ID: 2-s2.0-84954311265 01/18/2016       16 Citations
  • Streptomyces wadayamensis MppP Is a Pyridoxal 5'-Phosphate-Dependent L-Arginine α-Deaminase, γ-Hydroxylase in the Enduracididine Biosynthetic Pathway. (Han L, Schwabacher AW, Moran GR, Silvaggi NR) Biochemistry 2015 Dec 01;54(47):7029-40 PMID: 26551990 SCOPUS ID: 2-s2.0-84948457830 11/10/2015       30 Citations
  • Antitumor Activity of 3-Indolylmethanamines 31B and PS121912. (Guthrie ML, Sidhu PS, Hill EK, Horan TC, Nandhikonda P, Teske KA, Yuan NY, Sidorko M, Rodali R, Cook JM, Han L, Silvaggi NR, Bikle DD, Moore RG, Singh RK, Arnold LA) Anticancer Res 2015 Nov;35(11):6001-7 PMID: 26504023 PMCID: PMC4633305 SCOPUS ID: 2-s2.0-84946067038 10/28/2015       7 Citations
  • Sbi00515, a Protein of Unknown Function from Streptomyces bingchenggensis, Highlights the Functional Versatility of the Acetoacetate Decarboxylase Scaffold. (Mydy LS, Hoppe RW, Ochsenwald JM, Berndt RT, Severin GB, Schwabacher AW, Silvaggi NR) Biochemistry 2015 Jun 30;54(25):3978-88 PMID: 26039798 SCOPUS ID: 2-s2.0-84933533042 06/04/2015       3 Citations
  • Bacterial Renalase: Structure and Kinetics of an Enzyme with 2- and 6-Dihydro-β-NAD(P) Oxidase Activity from Pseudomonas phaseolicola. (Hoag MR, Roman J, Beaupre BA, Silvaggi NR, Moran GR) Biochemistry 2015 Jun 23;54(24):3791-802 PMID: 26016690 SCOPUS ID: 2-s2.0-84934954581 05/29/2015       18 Citations
  • The aspartate-less receiver (ALR) domains: distribution, structure and function. (Maule AF, Wright DP, Weiner JJ, Han L, Peterson FC, Volkman BF, Silvaggi NR, Ulijasz AT) PLoS Pathog 2015 Apr;11(4):e1004795 PMID: 25875291 PMCID: PMC4395418 SCOPUS ID: 2-s2.0-84929484388 04/16/2015       21 Citations
  • You are lost without a map: Navigating the sea of protein structures. (Lamb AL, Kappock TJ, Silvaggi NR) Biochim Biophys Acta 2015 Apr;1854(4):258-68 PMID: 25554228 PMCID: PMC5051661 SCOPUS ID: 2-s2.0-84921044740 01/03/2015       21 Citations
  • IDENTIFICATION OF VDR ANTAGONISTS AMONG NUCLEAR RECEPTOR LIGANDS USING VIRTUAL SCREENING. (Teske K, Nandhikonda P, Bogart JW, Feleke B, Sidhu P, Yuan N, Preston J, Goy R, Han L, Silvaggi NR, Singh RK, Bikle DD, Cook JM, Arnold LA) Nucl Receptor Res 2014;1 PMID: 25419525 PMCID: PMC4240308 11/25/2014    
  • Anticancer activity of VDR-coregulator inhibitor PS121912. (Sidhu PS, Teske K, Feleke B, Yuan NY, Guthrie ML, Fernstrum GB, Vyas ND, Han L, Preston J, Bogart JW, Silvaggi NR, Cook JM, Singh RK, Bikle DD, Arnold LA) Cancer Chemother Pharmacol 2014 Oct;74(4):787-98 PMID: 25107568 PMCID: PMC4177010 SCOPUS ID: 2-s2.0-84939871260 08/12/2014       19 Citations
  • Structural and functional characterization of MppR, an enduracididine biosynthetic enzyme from streptomyces hygroscopicus: functional diversity in the acetoacetate decarboxylase-like superfamily. (Burroughs AM, Hoppe RW, Goebel NC, Sayyed BH, Voegtline TJ, Schwabacher AW, Zabriskie TM, Silvaggi NR) Biochemistry 2013 Jul 02;52(26):4492-506 PMID: 23758195 PMCID: PMC3743547 SCOPUS ID: 2-s2.0-84880168001 06/14/2013       22 Citations
  • Evaluation of adamantane hydroxamates as botulinum neurotoxin inhibitors: synthesis, crystallography, modeling, kinetic and cellular based studies. (Šilhár P, Silvaggi NR, Pellett S, Čapková K, Johnson EA, Allen KN, Janda KD) Bioorg Med Chem 2013 Mar 01;21(5):1344-8 PMID: 23340139 PMCID: PMC3574188 SCOPUS ID: 2-s2.0-84873722654 01/24/2013       46 Citations
  • Binding energy and catalysis by D-xylose isomerase: kinetic, product, and X-ray crystallographic analysis of enzyme-catalyzed isomerization of (R)-glyceraldehyde. (Toteva MM, Silvaggi NR, Allen KN, Richard JP) Biochemistry 2011 Nov 22;50(46):10170-81 PMID: 21995300 PMCID: PMC3217161 SCOPUS ID: 2-s2.0-81255195348 10/15/2011       14 Citations
  • Chirality holds the key for potent inhibition of the botulinum neurotoxin serotype a protease. (Stowe GN, Silhár P, Hixon MS, Silvaggi NR, Allen KN, Moe ST, Jacobson AR, Barbieri JT, Janda KD) Org Lett 2010 Feb 19;12(4):756-9 PMID: 20092262 PMCID: PMC2821465 SCOPUS ID: 2-s2.0-76849114940 01/23/2010       30 Citations
  • Catalytic features of the botulinum neurotoxin A light chain revealed by high resolution structure of an inhibitory peptide complex. (Silvaggi NR, Wilson D, Tzipori S, Allen KN) Biochemistry 2008 May 27;47(21):5736-45 PMID: 18457419 SCOPUS ID: 2-s2.0-44349178037 05/07/2008       55 Citations
  • Structures of Clostridium botulinum Neurotoxin Serotype A Light Chain complexed with small-molecule inhibitors highlight active-site flexibility. (Silvaggi NR, Boldt GE, Hixon MS, Kennedy JP, Tzipori S, Janda KD, Allen KN) Chem Biol 2007 May;14(5):533-42 PMID: 17524984 SCOPUS ID: 2-s2.0-34248594822 05/26/2007       117 Citations
  • Double-lanthanide-binding tags for macromolecular crystallographic structure determination. (Silvaggi NR, Martin LJ, Schwalbe H, Imperiali B, Allen KN) J Am Chem Soc 2007 Jun 06;129(22):7114-20 PMID: 17497863 SCOPUS ID: 2-s2.0-34250217022 05/15/2007       74 Citations
  • Double-lanthanide-binding tags: design, photophysical properties, and NMR applications. (Martin LJ, Hähnke MJ, Nitz M, Wöhnert J, Silvaggi NR, Allen KN, Schwalbe H, Imperiali B) J Am Chem Soc 2007 Jun 06;129(22):7106-13 PMID: 17497862 SCOPUS ID: 2-s2.0-34250191079 05/15/2007       136 Citations
  • The X-ray crystal structures of human alpha-phosphomannomutase 1 reveal the structural basis of congenital disorder of glycosylation type 1a. (Silvaggi NR, Zhang C, Lu Z, Dai J, Dunaway-Mariano D, Allen KN) J Biol Chem 2006 May 26;281(21):14918-26 PMID: 16540464 SCOPUS ID: 2-s2.0-33744955343 03/17/2006       62 Citations
  • Inactivation of bacterial DD-peptidase by beta-sultams. (Llinás A, Ahmed N, Cordaro M, Laws AP, Frère JM, Delmarcelle M, Silvaggi NR, Kelly JA, Page MI) Biochemistry 2005 May 31;44(21):7738-46 PMID: 15909988 SCOPUS ID: 2-s2.0-19644380904 05/25/2005       33 Citations
  • Crystal structures of complexes between the R61 DD-peptidase and peptidoglycan-mimetic beta-lactams: a non-covalent complex with a "perfect penicillin". (Silvaggi NR, Josephine HR, Kuzin AP, Nagarajan R, Pratt RF, Kelly JA) J Mol Biol 2005 Jan 21;345(3):521-33 PMID: 15581896 SCOPUS ID: 2-s2.0-9644281538 12/08/2004       50 Citations
  • Toward better antibiotics: crystallographic studies of a novel class of DD-peptidase/beta-lactamase inhibitors. (Silvaggi NR, Kaur K, Adediran SA, Pratt RF, Kelly JA) Biochemistry 2004 Jun 08;43(22):7046-53 PMID: 15170342 SCOPUS ID: 2-s2.0-2642588180 06/02/2004       18 Citations
  • The crystal structure of phosphonate-inhibited D-Ala-D-Ala peptidase reveals an analogue of a tetrahedral transition state. (Silvaggi NR, Anderson JW, Brinsmade SR, Pratt RF, Kelly JA) Biochemistry 2003 Feb 11;42(5):1199-208 PMID: 12564922 SCOPUS ID: 2-s2.0-0037432016 02/05/2003       57 Citations
  • Structures of two kinetic intermediates reveal species specificity of penicillin-binding proteins. (McDonough MA, Anderson JW, Silvaggi NR, Pratt RF, Knox JR, Kelly JA) J Mol Biol 2002 Sep 06;322(1):111-22 PMID: 12215418 SCOPUS ID: 2-s2.0-0036965578 09/07/2002       80 Citations
  • A 1.2-A snapshot of the final step of bacterial cell wall biosynthesis. (Lee W, McDonough MA, Kotra L, Li ZH, Silvaggi NR, Takeda Y, Kelly JA, Mobashery S) Proc Natl Acad Sci U S A 2001 Feb 13;98(4):1427-31 PMID: 11171967 PMCID: PMC29273 SCOPUS ID: 2-s2.0-0035852621 02/15/2001       97 Citations
    • Last update: 07/30/2020