Faculty Collaboration Database

Postprandial Effects on ENaC-Mediated Sodium Absorption. Sci Rep 2019 Mar 12;9(1):4296

Date

03/14/2019

Pubmed ID

30862903

Pubmed Central ID

PMC6414683

DOI

10.1038/s41598-019-40639-x

Scopus ID

2-s2.0-85062847686 (requires institutional sign-in at Scopus site)   18 Citations

Abstract

Recent studies have suggested that postprandial increases in insulin directly contribute to reduced urinary sodium excretion. An abundance of research supports the ability of insulin to augment epithelial sodium channel (ENaC) transport. This study hypothesized that ENaC contributes to the increase in renal sodium reabsorption following a meal. To test this, we used fasted or 4 hour postprandial Sprague Dawley rats to analyze ENaC expression and activity. We also assessed total expression of additional sodium transporters (Na⁺-Cl^- cotransporter (NCC), Na⁺-K⁺-2Cl^- cotransporter (NKCC2), and Na⁺-K⁺-ATPase (NKA)) and circulating hormones involved in the renin-angiotensin-aldosterone system (RAAS). We found that after carbohydrate stimulus, ENaC open probability increased in split-open isolated collecting duct tubules, while ENaC protein levels remained unchanged. This was supported by a lack of change in phosphorylated Nedd4-2, an E3 ubiquitin ligase protein which regulates the number of ENaCs at the plasma membrane. Additionally, we found no differences in total expression of NCC, NKCC2, or NKA in the postprandial rats. Lastly, there were no significant changes in RAAS signaling between the stimulated and fasted rats, suggesting that acute hyperinsulinemia increases ENaC activity independent of the RAAS signaling cascade. These results demonstrate that insulin regulation of ENaC is a potential mechanism to preserve sodium and volume loss following a meal, and that this regulation is distinct from classical ENaC regulation by RAAS.

Author List

Blass G, Klemens CA, Brands MW, Palygin O, Staruschenko A

MESH terms used to index this publication - Major topics in bold

Animals
Blood Glucose
Epithelial Sodium Channels
Hyperinsulinism
Male
Postprandial Period
Rats
Rats, Sprague-Dawley
Renin-Angiotensin System
Sodium
Solute Carrier Family 12, Member 1
Solute Carrier Family 12, Member 3
Ubiquitin-Protein Ligases