A family of three mouse potassium channel genes with intronless coding regions. Science 1990 Feb 23;247(4945):973-5
Date
02/23/1990Pubmed ID
2305265DOI
10.1126/science.2305265Scopus ID
2-s2.0-0025098397 (requires institutional sign-in at Scopus site) 153 CitationsAbstract
To understand the molecular mechanisms responsible for generating physiologically diverse potassium channels in mammalian cells, mouse genomic clones have been isolated with a potassium channel complementary DNA, MBK1, that is homologous to the Drosophila potassium channel gene, Shaker. A family of three closely related potassium channel genes (MK1, MK2, and MK3) that are encoded at distinct genomic loci has been isolated. Sequence analysis reveals that the coding region of each of these three genes exists as a single uninterrupted exon in the mouse genome. This organization precludes the generation of multiple forms of the protein by alternative RNA splicing, a mechanism known to characterize the Drosophila potassium channel genes Shaker and Shab. Thus, mammals may use a different strategy for generating diverse K+ channels by encoding related genes at multiple distinct genomic loci, each of which produces only a single protein.
Author List
Chandy KG, Williams CB, Spencer RH, Aguilar BA, Ghanshani S, Tempel BL, Gutman GAAuthor
Calvin B. Williams MD, PhD Chief, Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAnimals
DNA
DNA Probes
Drosophila
Exons
Introns
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Nucleic Acid Hybridization
Potassium Channels
Restriction Mapping
