Targeted delivery of pharmacological agents into rat dorsal root ganglion. J Neurosci Methods 2009 Mar 15;177(2):397-402
Date
11/26/2008Pubmed ID
19027036Pubmed Central ID
PMC2873081DOI
10.1016/j.jneumeth.2008.10.029Scopus ID
2-s2.0-58149512891 (requires institutional sign-in at Scopus site) 29 CitationsAbstract
We sought an optimal method for targeted delivery into dorsal root ganglia (DRGs) for experimental studies, in terms of precision of delivery and avoidance of behavioral disturbances. We examined three approaches for injection into rat DRGs: percutaneous injection without surgical exposure, injection after deep exposure, and injection following deep exposure and partial laminectomy. Coomassie blue and Fast Blue were injected into DRGs for validation. At necropsy, the spread of Coomassie blue and Fast Blue was investigated under stereomicroscope and fluorescent microscope, respectively. We found that percutaneous approach did not provide any successful DRG injections. Deep exposure prior to intraganglionic injection provided variable results, but intraganglionic injection after deep exposure plus partial laminectomy was successful in 100% of attempts. Our subsequent skeletal analysis showed that the anatomical location of DRG is not compatible with successful DRG injection without surgical exposure. Neither of the methods using surgical exposure caused behavioral disturbances. Based on these results we conclude that partial laminectomy offers the most precise method of injecting DRG and does not produce behavioral evidence of nerve damage. Intraganglionic injection after deep exposure alone is less predictable, while percutaneous approaches only allow injection in the peripheral nerve.
Author List
Puljak L, Kojundzic SL, Hogan QH, Sapunar DAuthor
Quinn H. Hogan MD Professor in the Anesthesiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AmidinesAnimals
Coloring Agents
Drug Delivery Systems
Fluorescent Dyes
Ganglia, Spinal
Laminectomy
Microdissection
Microinjections
Microscopy, Fluorescence
Neuropharmacology
Neurosurgical Procedures
Rats
Rats, Sprague-Dawley
Rosaniline Dyes
Sensory Receptor Cells
Spine
Staining and Labeling
