Comprehensive Assessments of Energy Balance in Mice. Methods Mol Biol 2017;1614:123-146
Date
05/14/2017Pubmed ID
28500600Pubmed Central ID
PMC5582947DOI
10.1007/978-1-4939-7030-8_10Scopus ID
2-s2.0-85019176097 (requires institutional sign-in at Scopus site) 41 CitationsAbstract
Increasing evidence supports a major role for the renin-angiotensin system (RAS) in energy balance physiology. The RAS exists as a circulating system but also as a local paracrine/autocrine signaling mechanism in target tissues including the gastrointestinal tract, the brain, the kidney, and distinct adipose beds. Through activation of various receptors in these target tissues, the RAS contributes to the control of food intake behavior, digestive efficiency, spontaneous physical activity, and aerobic and anaerobic resting metabolism. Although the assortment of methodologies available to assess the various aspects of energy balance can be daunting for an investigator new to this area, a relatively straightforward array of entry-level and advanced methodologies can be employed to comprehensively and quantitatively dissect the effects of experimental manipulations on energy homeostasis. Such methodologies and a simple initial workflow for the use of these methods are described in this chapter, including the use of metabolic caging systems, bomb calorimetry, body composition analyzers, respirometry systems, and direct calorimetry systems. Finally, a brief discussion of the statistical analyses of metabolic data is included.
Author List
Grobe JLAuthor
Justin L. Grobe PhD Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBasal Metabolism
Body Composition
Calorimetry
Energy Metabolism
Mice
Renin-Angiotensin System
